可移植人髓母细胞瘤PUMC-MBT1瘤株的建立与鉴定

doi:10.16352/j.issn.1001-6325.2022.09.1339

基础医学与临床 ›› 2022, Vol. 42 ›› Issue (9): 1339-1343.doi: 10.16352/j.issn.1001-6325.2022.09.1339

可移植人髓母细胞瘤PUMC-MBT1瘤株的建立与鉴定

王世尊^1,2,3, 张丹^1,2, 葛明³, 冯海凉^1,2, 刘玉琴^1,2*

1. 中国医学科学院基础医学研究所北京协和医学院基础学院病理学系,北京 100005;
2. 中国医学科学院基础医学研究所北京协和医学院基础学院细胞中心,北京 100005;
3. 首都医科大学附属北京儿童医院国家儿童医学中心神经外科, 北京 100045

收稿日期:2021-09-26 修回日期:2021-11-25 出版日期:2022-09-05 发布日期:2022-09-02
通讯作者: liuyuqin@pumc.edu.cn
基金资助:
国家科技基础条件平台项目(NSTI-BMCR21);中国医学科学院医学与健康科技创新工程项目(2021-I2M-1-053)

Establishment and identification of a medulloblastoma transplantable tumor strain PUMC-MBT1

WANG Shi-zun^1,2,3, ZHANG Dan^1,2, GE Ming³, FENG Hai-liang^1,2, LIU Yu-qin^1,2*

1. Department of Pathology, Institute of Basic Medical Sciences CAMS, School of Basic Medicine PUMC, Beijing 100005;
2. Cell Resource Center, Institute of Basic Medical Sciences CAMS, School of Basic Medicine PUMC, Beijing 100005;
3. Department of Neurosurgery, Beijing Children Hospital, Capital Medical University, National Center for Children’s Health, Beijing 100045, China

Received:2021-09-26 Revised:2021-11-25 Online:2022-09-05 Published:2022-09-02

摘要/Abstract

摘要： 目的建立细胞系来源的异种种植肿瘤(CDX)体内可连续稳定传代的人髓母细胞瘤研究模型,并分析其生长和形态特点、病理特征。方法采用自建人髓母细胞瘤细胞系(PUMC-MB1)移植于SCID小鼠右侧腋窝,接种细胞数为5×10⁶个/只。当细胞系来源的异种种植肿瘤(CDX)长径增长至1.5 cm时处死小鼠,收集肿瘤组织并进行体内传代操作,重复传代10次,于第10代时观察肿瘤生长特点及荷瘤小鼠的生存时间,收集肿瘤进行病理分析。结果 CDX来源的人髓母细胞瘤瘤株已在体内连续传代至10代(命名为PUMC-MBT1),成瘤率为100%,在接种第14天左右可触及皮下结节,第29天移植瘤长径达到1.5 cm左右,荷瘤小鼠平均寿命50 d。病理学检查结果显示移植瘤为经典型髓母细胞瘤,Ki-67阳性(80%~90%),Syn阳性(40%~50%),NeuN阳性(20%~30%)。结论本课题组成功建立了人髓母细胞瘤移植瘤瘤株PUMC-MBT1,造模简单便捷,成瘤率高,病理学性质稳定,保持了经典型髓母细胞瘤的特性,为髓母细胞瘤研究提供了更多的体内研究模型。

关键词: 髓母细胞瘤, 细胞系来源的异种种植肿瘤(CDX), 瘤株

Abstract: Objective To establish a transplantable tumor strain of medulloblastoma in vivo from cell-line-derived xenograft (CDX) and to analyze the growth and pathological characteristics of the transplanted tumor model. Methods A previously established medulloblastoma cell line(PUMC-MB1) was utilized to construct CDX. The cultured cell suspension was injected into the right axilla of SCID mice subcutaneously with dosage as 5×10⁶ cells/mouse. The mice were sacrificed when the diameter of CDX reached around 1.5 cm. The xenografts were harvested and transplanted to other mice with the same number of cells. The process was repeated 10 times. The growth characteristics of transplanted medulloblastoma and survival curve of tumor-bearing mice at passage 10 were observed. The pathological characteristics of transplanted tumor were analyzed. Results The transplantable strain of medullo- blastoma with stable in vivo properties was named PUMC-MBT1. The successful rate of tumor transplantation was 100% at passage 10. The subcutaneous mass could be palpated 14 days after injection. The diameters of subcutaneous xenograft could reach about 1.5 cm 29 days after injection. Tumor-bearing mice died 50 days after injection. HE sections demonstrated that the transplanted PUMC-MBT1 belonged to classic medulloblastoma and the positive rate of Ki-67, Syn and NeuN were 80%-90%, 40%-50% and 20%-30% respectively. Conclusions A successful animal model of medulloblastoma is established with easy manipulation, characteristic features of classic medulloblastoma, so it is a potential technology for in vivo research.

Key words: medulloblastoma, cell-line-derived xenograft (CDX), tumor strain

中图分类号:

R739.41

王世尊, 张丹, 葛明, 冯海凉, 刘玉琴. 可移植人髓母细胞瘤PUMC-MBT1瘤株的建立与鉴定[J]. 基础医学与临床, 2022, 42(9): 1339-1343.

WANG Shi-zun, ZHANG Dan, GE Ming, FENG Hai-liang, LIU Yu-qin. Establishment and identification of a medulloblastoma transplantable tumor strain PUMC-MBT1[J]. Basic & Clinical Medicine, 2022, 42(9): 1339-1343.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: http://journal11.magtechjournal.com/Jwk_jcyxylc/CN/10.16352/j.issn.1001-6325.2022.09.1339

http://journal11.magtechjournal.com/Jwk_jcyxylc/CN/Y2022/V42/I9/1339

参考文献 12

[1]	Orr BA. Pathology, diagnostics, and classification of medulloblastoma[J]. Brain Pathol, 2020, 30: 664-678.
[2]	漆松涛. 重视我国小儿神经外科的建设与发展[J]. 中华神经外科杂志, 2019, 35: 757-759.
[3]	Roussel MF, Stripay JL. Modeling pediatric medulloblastoma[J]. Brain Pathol, 2020, 30: 703-712.
[4]	马磊, 陈维英, 施又丹, 等. 原花青素对髓母细胞瘤Daoy细胞增殖和凋亡的影响[J]. 基础医学与临床, 2015, 35: 929-933.
[5]	Ballabio C, Anderle M, Gianesello M, et al. Modeling medulloblastoma in vivo and with human cerebellar organoids[J]. Nat Commun, 2020, 11: 583. doi: 10.1038/s41467-019-13989-3.
[6]	Dietl S, Schwinn S, Dietl S, et al. MB3W1 is an orthotopic xenograft model for anaplastic medulloblastoma displaying cancer stem cell- and group 3-properties[J]. BMC Cancer, 2016, 16: 115. doi: 10.1186/s12885-016-2170-z.
[7]	Badodi S, Marino S, Guglielmi L. Establishment and culture of patient-derived primary medulloblastoma cell lines[J]. Methods Mol Biol, 2019, 1869: 23-36.
[8]	Shiraishi R, Kawauchi D. Epigenetic regulation in medulloblastoma pathogenesis revealed by genetically engineered mouse models[J]. Cancer Sci, 2021, 112: 2948-2957.
[9]	Ramaswamy V, Remke M, Bouffet E, et al. Risk stratification of childhood medulloblastoma in the molecular era: the current consensus[J]. Acta Neuropathol, 2016, 131: 821-831.
[10]	Qi L, Kogiso M, Du Y, et al. Impact of SCID mouse gender on tumorigenicity, xenograft growth and drug-response in a large panel of orthotopic PDX models of pediatric brain tumors[J]. Cancer Lett, 2020, 493: 197-206.
[11]	Okada S, Vaeteewoottacharn K, Kariya R. Application of highly immunocompromised mice for the establishment of patient-derived xenograft (PDX) models[J]. Cells, 2019, 8:889. doi: 10.3390/cells8080889
[12]	Ben-david U, Ha G, Tseng YY, et al. Patient-derived xenografts undergo mouse-specific tumor evolution[J]. Nat Genet, 2017, 49: 1567-1575.

可移植人髓母细胞瘤PUMC-MBT1瘤株的建立与鉴定

Establishment and identification of a medulloblastoma transplantable tumor strain PUMC-MBT1

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献 12

相关文章 2

编辑推荐

Metrics

本文评价

[1]	吴高锒赵赋张晶李春德牛亚梅刘丕楠佟伟民. YTHDC1基因在小鼠小脑发育及人髓母细胞瘤组织中的动态表达[J]. 基础医学与临床, 2019, 39(7): 1001-1006.
[2]	王月圆高云周汪兆琦沈岩佟伟民. 小鼠髓母细胞瘤细胞系的建立与鉴定[J]. 基础医学与临床, 2009, 29(7): 737-741.