HMGB1减弱丹参酮ⅡA对大鼠心肌缺血/再灌注损伤的缓解作用

doi:10.16352/j.issn.1001-6325.2022.09.1333

基础医学与临床 ›› 2022, Vol. 42 ›› Issue (9): 1333-1338.doi: 10.16352/j.issn.1001-6325.2022.09.1333

HMGB1减弱丹参酮ⅡA对大鼠心肌缺血/再灌注损伤的缓解作用

钟金鹏¹, 杨莉², 王辉波¹, 陈红健¹, 李金伟¹, 文明洪¹, 吕云波^1*

1. 三峡大学人民医院宜昌市第一人民医院心内科, 湖北宜昌 443000;
2. 三峡大学人民医院宜昌市第一人民医院神经内科, 湖北宜昌 443000

收稿日期:2021-10-11 修回日期:2021-12-31 出版日期:2022-09-05 发布日期:2022-09-02
通讯作者: 62435582@qq.com
基金资助:
国家自然科学基金(82100270);湖北省卫生健康委员会联合基金(WJ2019H511)

HMGB1 attenuates the mitigative effect to myocardial ischemia-reperfusion injury by tanshinone ⅡA in rats

ZHONG Jin-peng¹, YANG Li², WANG Hui-bo¹,CHEN Hong-jian¹, LI Jin-wei¹, WEN Ming-hong¹, LYU Yun-bo^1*

1. Department of Cardiology, the People's Hospital of China Three Gorges University, Yichang 443000, China;
2. Department of Neurology, the People's Hospital of China Three Gorges University, Yichang 443000, China

Received:2021-10-11 Revised:2021-12-31 Online:2022-09-05 Published:2022-09-02

摘要/Abstract

摘要： 目的研究外源性高迁移率族蛋白B1(HMGB1)对丹参酮ⅡA(TSA)缓解大鼠心肌缺血/再灌注(I/R)损伤的影响。方法将大鼠分为假手术(sham)组、I/R组(结扎前降支冠脉)、TSA组(I/R+静脉注射TSA 10 mg/kg)、TSA+HMGB1组(I/R+静脉注射TSA 10 mg/kg+腹腔注射重组HMGB1 100 μg/kg)。再灌注24 h后,用ELISA测定肌酸激酶同工酶(CK-MB)、TCC测心梗面积,RT-qPCR及Western blot测定炎性指标表达。结果 I/R组与sham组比较,CK-MB升高(P＜0.01),心肌梗死面积增大,白介素-6(IL-6)、白介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)的转录水平增加(均P＜0.01),HMGB1、NOD样受体蛋白3(NLRP3)和胱冬肽酶-1(caspase-1)表达增加(均P＜0.05);TSA组与I/R组比较,CK-MB下降(P＜0.01),心梗面积缩小(P＜0.01),IL-6、IL-1β和TNF-α的转录水平下降(均P＜0.05),HMGB1、NLRP3、caspase-1和核因子-κB(NF-κB)表达减少(均P＜0.01);而TSA+HMGB1组与TSA组比较,CK-MB升高(P＜0.01),心肌梗死面积增大(P＜0.01),IL-6、IL-1β和TNF-α转录水平增加(均P＜0.01),HMGB1、NLRP3、caspase-1和NF-κB表达增加(均P＜0.05)。结论外源性HMGB1通过上调炎性因子表达减弱TSA缓解大鼠的心肌I/R损伤。

关键词: 高迁移率族蛋白B1, 丹参酮ⅡA, 心肌缺血/再灌注损伤, 炎性因子

Abstract: Objective To study the influence of exogenous high-mobility group box 1(HMGB1) on the mitigative effect of tanshinone ⅡA (TSA) to myocardial ischemia-reperfusion (I/R) injury. Methods Rats were divided into sham, I/R (ligation of left anterior descending coronary artery), TSA (I/R + TSA intravenous injection) and TSA+HMGB1 (I/R+TSA intravenous injection + recombination HMGB1 intraperitoneal injection) groups. Serum CK-MB was measured by ELISA. Myocardial infarct area was measured by TCC. The expression of inflammatory indicators were measured by RT-qPCR and Western blot. Results Compared with sham group, CK-MB, myocardial infarct area, transcription level of IL-6, IL-1β and TNF-α, expression of HMGB1, NLRP3 and caspase-1 all increased in I/R group(P＜0.05). Compared with I/R group, CK-MB, myocardial infarct area, transcription levels of IL-6, IL-1β and TNF-α, expressions of HMGB1, NLRP3, caspase-1 and NF-κB decreased in TSA group (P＜0.05). However, compared with TSA group, CK-MB, myocardial infarct area, transcription levels of IL-6, IL-1β and TNF-α, expressions of HMGB1, NLRP3, caspase-1 and NF-κB increased in TSA+HMGB1 group(P＜0.05). Conclusions Exogenous HMGB1 attenuates the mitigative effect of tanshinone ⅡA to rat myocardial I/R injury through up-regulation the expressions of inflammatory factors.

Key words: high-mobility group box 1, tanshinone ⅡA, myocardial ischemia-reperfusion injury, inflammatory factor

中图分类号:

R543.3

钟金鹏, 杨莉, 王辉波, 陈红健, 李金伟, 文明洪, 吕云波. HMGB1减弱丹参酮ⅡA对大鼠心肌缺血/再灌注损伤的缓解作用[J]. 基础医学与临床, 2022, 42(9): 1333-1338.

ZHONG Jin-peng, YANG Li, WANG Hui-bo,CHEN Hong-jian, LI Jin-wei, WEN Ming-hong, LYU Yun-bo. HMGB1 attenuates the mitigative effect to myocardial ischemia-reperfusion injury by tanshinone ⅡA in rats[J]. Basic & Clinical Medicine, 2022, 42(9): 1333-1338.

参考文献 15

[1]	Wei B, Li WW, Ji J, et al. The cardioprotective effect of sodium tanshinone IIA sulfonate and the optimizing of therapeutic time window in myocardial ischemia/reperfusion injury in rats[J]. Atherosclerosis, 2014,235:318-327.
[2]	Hu H, Zhai C, Qian G, et al. Protective effects of tanshinone IIA on myocardial ischemia reperfusion injury by reducing oxidative stress, HMGB1 expression, and inflammatory reaction[J]. Pharm Biol, 2015,53:1752-1758.
[3]	Raucci A, Di MS, Scavello F, et al. The Janus face of HMGB1 in heart disease: a necessary update[J]. Cell Mol Life Sci, 2019,76:211-229.
[4]	Lin Y, Chen L, Li W, et al. Role of high-mobility group box-1 in myocardial ischemia/reperfusion injury and the effect of ethyl pyruvate[J]. Exp Ther Med, 2015,9:1537-1541.
[5]	Abarbanell AM, Hartley JA, Herrmann JL, et al. Exogenous high-mobility group box 1 improves myocardial recovery after acute global ischemia/reperfusion injury[J]. Surgery, 2011,149:329-335.
[6]	Wang JG, Bondy SC, Zhou L, et al. Protective effect of tanshinone IIA against infarct size and increased HMGB1, NFκB, GFAP and apoptosis consequent to transient middle cerebral artery occlusion[J]. Neurochem Res, 2014,39:295-304.
[7]	Li X, Wu Y, Zhang W, et al. Pre-conditioning with tanshinone IIA attenuates the ischemia/reperfusion injury caused by liver grafts via regulation of HMGB1 in rat Kupffer cells[J]. Biomed Pharmacother, 2017,89:1392-1400.
[8]	Andrassy M, Volz HC, Igwe JC, et al. High-mobility group box-1 in ischemia-reperfusion injury of the heart[J]. Circulation, 2008,117:3216-3226.
[9]	Andrassy M, Volz HC, Riedle N, et al. HMGB1 as a predictor of infarct transmurality and functional recovery in patients with myocardial infarction[J]. J Intern Med, 2011,270:245-253.
[10]	Tian Y, Pan D, Chordia MD, et al. The spleen contributes importantly to myocardial infarct exacerbation during post-ischemic reperfusion in mice via signaling between cardiac HMGB1 and splenic RAGE[J]. Basic Res Cardiol, 2016,111:62-84.
[11]	van Hout GP, Bosch L, Ellenbroek GH, et al. The selective NLRP3-inflammasome inhibitor MCC950 reduces infarct size and preserves cardiac function in a pig model of myocardial infarction[J]. Eur Heart J, 2017,38:828-836.
[12]	Dong LY, Chen F, Xu M, et al. Quercetin attenuates myocardial ischemia-reperfusion injury via downregulation of the HMGB1-TLR4-NF-κB signaling pathway[J]. Am J Transl Res, 2018,10:1273-1283.
[13]	Loukili N, Rosenblatt-Velin N, Li J, et al. Peroxynitrite induces HMGB1 release by cardiac cells in vitro and HMGB1 upregulation in the infarcted myocardium in vivo[J]. Cardiovasc Res, 2011,89:586-594.
[14]	Hu X, Jiang H, Cui B, et al. Preconditioning with high mobility group box 1 protein protects against myocardial ischemia-reperfusion injury[J]. Int J Cardiol, 2010,145:111-112.
[15]	Xue J, Suarez JS, Minaai M, et al. HMGB1 as a therapeutic target in disease[J]. J Cell Physiol, 2021,236:3406-3419.

HMGB1减弱丹参酮ⅡA对大鼠心肌缺血/再灌注损伤的缓解作用

HMGB1 attenuates the mitigative effect to myocardial ischemia-reperfusion injury by tanshinone ⅡA in rats

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献 15

相关文章 15

编辑推荐

Metrics

本文评价

[1]	苏拾香, 王语阳, 覃宗帅, 黄桂香, 徐键, 岑兰英, 覃月秋. miR-142-3p通过调控Hmgb1抑制雨蛙素诱导的大鼠胰腺外分泌细胞系AR42J凋亡[J]. 基础医学与临床, 2024, 44(1): 23-30.
[2]	李晓娜, 齐先梅, 张田甜, 王婧. 沉默Fcgr3降低SiO₂诱导的小鼠肺泡巨噬细胞系MH-S炎性因子表达[J]. 基础医学与临床, 2023, 43(6): 904-908.
[3]	孙莹, 杨治安, 何瑶, 余勤. HMGB1信号通路在支气管哮喘中作用的研究进展[J]. 基础医学与临床, 2023, 43(4): 690-694.
[4]	张梦瑶, 牛姝, 蔡静, 张立香, 赵志刚. 丹参酮ⅡA减轻高糖诱导的小鼠足细胞系PMC-5凋亡和氧化应激反应[J]. 基础医学与临床, 2023, 43(2): 277-282.
[5]	马聪聪, 刘洋, 吕洋, 王海萍. 线粒体自噬在心肌缺血/再灌注损伤中的作用研究进展[J]. 基础医学与临床, 2023, 43(11): 1723-1727.
[6]	崔艺耀, 王峰, 吕晓烁, 刘春全, 胥凯凯, 崔永. 盐酸氨溴索减轻胃液误吸导致的大鼠急性肺损伤[J]. 基础医学与临床, 2022, 42(8): 1250-1254.
[7]	余子强, 徐久平, 詹长生, 邵明峰, 邹建安. 输尿管软镜碎石与经皮肾镜碎石对3 cm以下肾结石疗效和炎性反应的影响[J]. 基础医学与临床, 2022, 42(8): 1259-1262.
[8]	努尔曼·阿卜杜克力木, 韩正风, 马艳. 医院-社区-家庭一体化康复模式对老年衰弱综合征患者的影响[J]. 基础医学与临床, 2022, 42(4): 640-645.
[9]	石丹丹, 蒋素芳, 王茜, 赵鹏博, 刘雅. IL-6反式信号传导通路抑制剂sgp130Fc抑制脓毒症小鼠炎性反应及调节免疫细胞[J]. 基础医学与临床, 2022, 42(12): 1895-1899.
[10]	李嘉晋, 刘军, 何松于, 谌浩云. 下调miR-27a减轻脓毒症模型大鼠肺损伤[J]. 基础医学与临床, 2022, 42(11): 1704-1708.
[11]	树梦萱, 袁方晨, 周梦琪, 涂世盐, 许玥, 秦红岩. 结肠炎诱发肝脏屏障功能障碍的研究进展[J]. 基础医学与临床, 2022, 42(1): 149-153.
[12]	王布, 袁胜芳, 张长洪, 苑程, 黄攀登, 张志华, 赵建清. eEF2K沉默联合丹参酮ⅡA磺酸钠协同抑制人肺腺癌细胞系A549增殖[J]. 基础医学与临床, 2022, 42(1): 106-113.
[13]	房婷, 李响, 刘洁, 王皓. 鼠李糖乳杆菌通过调节肠道微生物群缓解造血干细胞移植后肝脏移植物抗宿主病[J]. 基础医学与临床, 2021, 41(9): 1303-1308.
[14]	宫丹丹, 隋春兴, 石晨, 李月, 姜晓东. 沉默miR-26a减轻缺血/再灌注大鼠心肌损伤[J]. 基础医学与临床, 2021, 41(9): 1285-1290.
[15]	李倩, 刘纯华, 王晨怡, 谈娇娇, 马玉萍, 吕海宏. 炎性因子调控1型糖尿病血管内皮细胞功能的研究进展[J]. 基础医学与临床, 2021, 41(10): 1497-1501.