基础医学与临床 ›› 2022, Vol. 42 ›› Issue (3): 461-466.doi: 10.16352/j.issn.1001-6325.2022.03.024

• 研究论文 • 上一篇    下一篇

SLC4A10表达水平对肝癌患者预后的影响

黄容1, 张键1, 车旭2*   

  1. 1.中国科学院深圳先进技术研究院 生物医学与生物技术研究所 能量代谢与生殖研究中心,广东 深圳 518055;
    2.国家癌症中心 国家肿瘤临床医学研究中心 中国医学科学院 北京协和医学院 肿瘤医院深圳医院 肝胆外科,广东 深圳 518116
  • 收稿日期:2021-05-28 修回日期:2021-10-09 出版日期:2022-03-05 发布日期:2022-03-04
  • 通讯作者: * dr.chex@hotmail.com
  • 基金资助:
    深圳市“医疗卫生三名工程”项目(SZSM202011010); 深圳市高水平医院建设专项经费资助

Effect of SLC4A10 expression on prognosis of patient with hepatocellular carcinoma

HUANG Rong1, ZHANG Jian1, CHE Xu2*   

  1. 1. Center for Energy Metabolism and Reproduction, Institute of Biomedicine and Biotechnology,Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences,Shenzhen 518055;
    2. Department of Hepatobiliary Surgery, National Cancer Center,National Clinical Research Center for Cancer,Cancer Hospital & Shenzhen Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College, Shenzhen 518116, China
  • Received:2021-05-28 Revised:2021-10-09 Online:2022-03-05 Published:2022-03-04
  • Contact: * dr.chex@hotmail.com

摘要: 目的 通过生物信息学(生信学)分析在肝细胞癌(HCC)中SLC4A10的表达对HCC患者预后的影响,初步验证并探讨其意义。方法 通过TCGA数据库,分析SLC4A10在HCC组织与正常组织间的差异表达,并利用实时定量PCR进行验证。运用Kaplan-Meier(K-M)曲线分析SLC4A10的表达量对HCC患者预后的影响;通过单因素与多因素回归分析SLC4A10与HCC患者总生存期(CS)和无病生存期(DFS)的相关性;通过PPI数据库构建蛋白互作网络并进行功能富集分析,探索SLC4A10在HCC中的调控机制。结果 通过生信学分析发现,SLC4A10在HCC组织中的表达显著低于正常组织;SLC4A10 mRNA表达降低与晚期临床病理参数显著相关,并降低HCC患者的CS和DFS;功能富集分析显示SLC4A10参与的重要的生物过程包括跨上皮转运、细胞体积稳态和钠离子跨膜导入。结论 通过生信学分析提示SLC4A10在HCC中表达降低,其高表达利于HCC患者的预后,可能是HCC的潜在预后标志物或治疗靶点。

关键词: 肝细胞肝癌(HCC), SLC4A10, 预后, 标志物

Abstract: Objective To analyze the effect of SLC4A10 expression on the prognosis of patients with hepatocellular carcinoma(HCC)by bioinformatics, and to confirm its expression in HCC tissue and normal tissue. Methods The expression of SLC4A10 in HCC and in normal tissues was analyzed by TCGA database, and verified by real-time quantitative PCR. Kaplan-Meier(K-M) curve was applied to assess survival profile . Univariate and multivariate Cox regression analysis was applied to address the influence of independent factors on overall survival(OS)and disease free survival (DFS). PPI database was used to construct protein interaction network and function enrichment analysis was used to explore the regulatory mechanism of SLC4A10 in HCC. Results Significantly decreased transcriptional SLC4A10 expression was found in HCC samples. Decreased SLC4A10 mRNA expression was significantly associated with advanced pathological parameters and with shorter OS and DFS in TCGA cohorts. Functional annotations indicated that SLC4A10 was involved in the most significant hallmarks including transepithelial transport, cell volume homeostasis and sodium ion import across plasma membrane. Conclusions The decreased SLC4A10 expression is significantly correlated with aggressive progression and poor survival of HCC patients. These data suggest that SLC4A10 may act as a promising prognostic marker or therapeutic target in HCC.

Key words: hepatocellular carcinoma(HCC), SLC4A10, prognosis, marker

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