Basic & Clinical Medicine

Methylglyoxal reduces cell viability and mitochondrion membrane potential of human brain microvascular endothelial cell line hCMEC/D3

LI Tingting, HAN Zhenping, GAO Li, WANG Siyi, ZHONG Kai, HUANG Ping, YAN Jieping

2023, 43(11): 1631-1635. doi:10.16352/j.issn.1001-6325.2023.11.1631

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Objective To explore the effects and mechanism of methylglyoxal (MGO) in human brain microvascular endothelial cell line (hCMEC/D3). Methods The hCMEC/D3 cells were incubated with MGO(0.25, 0.50, 0.75, 1.00 and 1.25 mmol/L) for 24 h. Cell viability was detected by CCK-8 assay. Cellular LDH and SOD activities were determined by commercially available kits. The change of mitochondrion membrane potential was assessed by JC-1 probe. MitoSOX probe was used to observe mitochondrial reactive oxygen species (mROS) release. Results Compared with control, cell viability of hCMEC/D3 treated with MGO decreased in a concentration-dependent manner (P＜0.01). Increased LDH activity and decreased SOD activity were observed with MGO exposure(P＜0.01). The decrease of mitochondrion membrane potential and the excessive increase of mROS were induced after MGO exposure for 12 h, which were detected by JC-1 or MitoSOX probe. Conclusions MGO reduces cell viability and mitochondria membrane potential in hCMEC/D3 cells with excessive mROS generation.

Effect of sildenafil on NLRP3/caspase-1 pathway in the aorta vascular tissue of rat models with erectile dysfunction

ZHANG Xijia, ZHU Bingbing, YANG Chengxia, NIU Lipan, LIU Fengxia

2023, 43(11): 1636-1641. doi:10.16352/j.issn.1001-6325.2023.11.1636

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Objective To investigate the effect of sildenafil (Sil) on the expression of NLRP3/caspase-1 pathway in the aorta vascular tissue of erectile dysfunction (ED) rat models. Methods ED rat model was established and randomly divided into ED group and Sil group. Another 10 rats were selected as control group. After intragastric administration of Sil(20 mg /kg, once a day for 2 weeks), HE staining was used to observe the morphological change of aorta. Aortic fibrosis was detected by Masson staining. The content and distribution of interleukin-1β (IL-1β) and endothelial nitric oxide synthase (eNOS) in aorta were determined by immunohistochemistry. RT-qPCR and Western blot were used to detect the expression of NLRP3, caspase-1, GSDMD, IL-1β and eNOS in the aorta. Results In ED group, there were pathological changes in aortic morphology, more local exfoliation of endothelial cells and obvious fibrosis. The mRNA and protein expressions of NLRP3, caspase-1, GSDMD and IL-1β were increased, and the mRNA and protein expressions of eNOS were decreased in the aorta of the rats in the experimental group(P＜0.05). Compared with the ED group, the aortic morphological changes of the Sil group were improved, the local endothelial cell shedding was reduced, and the fibrosis was alleviated. The mRNA and protein expression of NLRP3, caspase-1, GSDMD and IL-1β decreased, while the mRNA and protein expression of eNOS increased in aorta(P＜0.05). Conclusions The expression of NLRP3/caspase-1 pathway protems in the aorta vescular tissue of rats with ED is up-regulated by Sil, which may improve the inflammatory response, vascular fibrosis and endothelial dysfunction by inhibiting the NLRP3/caspase-1 pathway in the aorta vascular tissue of rats with ED.

LncRNA (HOTAIR) /miR-206/TAGLN2 signal axis regulates the invasion of papillarythyroid carcinoma cells

HOU Lijie, MA Chengxu, WANG Jingyu, TANG Xulei, FU Songbo

2023, 43(11): 1642-1647. doi:10.16352/j.issn.1001-6325.2023.11.1642

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Objective To investigate the molecular mechanism of lncRNA (HOTAIR)/miR-206/TAGLN2 signaling axis in regulating the invasion of papillary thyroid carcinoma cells line TPC-1. Methods The papillary thyroid carcinoma(PTC) and paracancerous tissues were collected from the First Hospital of Lanzhou University, and the expression of lncRNA (HOTAIR), miR-206 and TAGLN2 in PTC tissues as well as paracancerous tissues was detected by RT-qPCR and Western blot; The thyroid papillary cancer cells with miR-206 over-expression and HOTAIR under-expression were constructed to detect the expression of target gene and its effect on the invasion of TPC-1 cells by Transwell assay. Results Compared with the adjacent tissue, the expression of HOTAIR in papilla-rythyroid cancer tissue was increased by 2.48 times. miR-206-5p was decreased down to 0.6,and the expression of TAGLN2 was increased (P＜0.05). After over-expression of miR-206, the protein expression of TAGLN2 was decreased in TPC-1 cell, and the invasion of TPC-1 cells was inhibited (P＜0.05). Knockdown of HOTAIR increased expression of miR-206 in TPC-1 cells and decreased the expression of TAGLN2. The Transwell assay showed that the invasion of TPC-1 cells was significantly reduced (P＜0.05). Conclusions In PTC, reducing the expression of HOTAIR results in over-expression of miR-206 then further inhibits the expression of TAGLN2. The biological outcome is inhibition of invasion of thyroid cancer.

Gallic acid alleviates airway inflammation induced by cigarette smoke in mice

ZHAO Fang, ZHAO Qing, LIU Weilin, LI Fei

2023, 43(11): 1648-1654. doi:10.16352/j.issn.1001-6325.2023.11.1648

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Objective To investigate the effect of gllic acid (GA) on the pulmonary inflammatory response associated with chronic obstructive pulmonary disease (COPD) induced by cigarette smoking (CS) in mice. Methods The mouse model induced by CS was constructed. The counting number of inflammatory cells and the level of proinflammatory cytokines in bronchoalveolar lavage fluid were analyzed. The ROS, GSH, MDA and protein carbonyl activity in lung tissue homogenate were determined by commercially available kits. Western blot was used to analyze IL-13/STAT6 pathway. Results GA inhibited SC-induced inflammatory cell infiltration (neutrophils, lymphocytes, and macrophages) and decreased the level of pro-inflammatory cytokines (IL-6, TNF-α, and IL-1β). GA alleviated the redox imbalance and CS-induced airway inflammation in lung tissue of CS exposed mice. The later might be mediated by by inhibiting IL-13/STAT6 signaling pathway. Conclusions GA effectively modulates pulmonary inflammation and oxidative stress in mice associated with COPD pathogenesis.

Influencing factors and correlation analysis of population with high risk of cardiovascular diseases

SHEN Xiafen, CAI Qiang, YU Wei, XU Xiaohui

2023, 43(11): 1655-1661. doi:10.16352/j.issn.1001-6325.2023.11.1655

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Objective To highlight the impact of behavioral and non-behavioral factors on cardiovascular health, disease prevention and management. Methods Cox regression and forest plots were used to assess and demonstrate the relationship between their behavioral and non-behavioral factors and cardiovascular disease (CVD) risk in a study population of 20 745 people at high cardiovascular risk from Zhejing province. Apriori algorithm was used to analyze the correlation between behavioral factors and non-behavioral factors for people with at least one of the greatest risk behavioral factors. Results The incidence of cardiovascular risk in Zhejiang province was positively related to age and inversely related to education level of target population. The higher risk was identified in in male and population in urban citities as compared to female and the people in rural areas; The risk was positively related to smoking intensity and showed a U-shaped association with alcohol intake; The incidence was lowest in those who were mainly standing during routine work; The risk was lower in those who had a clear distinction between busy and idle farming activity in farmers; Men who were engaged in general physical labor and never smoked nor drank high dose of alcohol; Women who never smoked and drank, but did not work and at home were important factors related to the higher cardiovascular risk rate. Conclusions There are a wide range of factors affecting the high-risk population of cardiovascular disease, and there is a certain correlation among these factors.

Detection and clinical application of VAR2 synthetic peptide specific binding to circulating tumor cells

YU Hong, DING Lijie, LIU Houcong, WANG Jidong, DU Jihui

2023, 43(11): 1662-1667. doi:10.16352/j.issn.1001-6325.2023.11.1662

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Objective To investigate the detection and clinical application studies based on malaria protein VAR2CSA(VAR2) synthetic peptide specifically binding to circulating tumor cells (CTCs). Methods The peptide sequence of the functional structural domain of malaria protein VAR2CSA was selected to synthesize VAR2 that enriched CTCs based on nano-microfluidic technology, and used VAR2 synthetic peptide to detect enriched cells to establish a novel detection protocol for CTCs. Cellular immuno-fluorescence staining, flow cytometry and tumor cell doping recovery assays were used to analyze the specific binding and recovery efficiency of VAR2 synthetic peptide with tumor cells of different tissue sources and compare with immuno-staining-fluorescence in situ hybridization (imFISH) assay. Twenty-two colorectal cancer patients and twenty-two healthy individuals were selected, and the VAR2 synthetic peptide was used to detect peripheral blood CTCs in both. The VAR2 synthetic peptide was used to compare the positive detection rate of CTCs in patients with different TNM stages. Results The VAR2 synthetic peptide bound specifically to tumor cells collected from different tissue sources of human colon cancer cell line SW620, human breast cancer cell line MCF7 and human esophageal squamous carcinoma cell line KYSE180.The VAR2 synthetic peptide detected 100 SW620, MCF7 and KYSE180 tumor cells mixed into 3 mL of peripheral blood with recovery rates of 82.8%±8.41%, 56.2%±3.57%, 86.6%±8.19%, which were not significantly different from the imFISH assay. The VAR2 synthetic peptide assay recoveries of 50, 100, and 200 SW620 tumor cells were 80.7%, 82.8%, and 84.7%, respectively, which did not correlate significantly with the number of adulterated tumor cells. The VAR2 synthetic peptide detected 63.6% positive CTCs in peripheral blood samples from patients with clinical colorectal cancer, which was higher than the 0.0% positive CTCs rate in the group of healthy subjects, and also correlated with depth of invasion, lymph node metastasis, distant metastasis, and clinical stage (P＜0.05). Conclusions The VAR2-based synthetic peptide may be applied in specific and non-antibody dependent CTCs detection in different tissue samples and so has good clinical application value for clinical staging and severity evaluation of tumor patients.

Urotensin Ⅱ receptor antagonist(urantide) alleviates renal injury in rats with atherosclerosis

LIU Yifei, WANG Xinyu, WANG Jiahui, DU Xiaobing, WANG Tu, ZHAO Juan

2023, 43(11): 1668-1672. doi:10.16352/j.issn.1001-6325.2023.11.1668

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Objective To explore the mechanism of action of urantide in ameliorating renal injury caused by atherosclerosis(AS). Methods The rats were divided into normal group, model group which were treated with ip VitD3 150 U/(kg·d^-1) for 3 consecutive days, and fed with high-fat special diet, with an experimental period of 6 weeks], simvastatin group [5 μg/(kg·d^-1) for 14 consecutive days] and intervention model group [urantide 30 μg/(kg·d^-1),7 d and 14 d]. Microscopy was performed for observing pathological changes in the kidney and evaluation of the degree of renal tissue damage. RT-qPCR and immuno-histochemical staining microscopy was used to detect the expression of related genes and proteins. Results Compared to the normal group, the model group showed glomerular atrophy in the renal tissue and adhesion with surrounding tissues. The experiment found a series of changes in renal tubular epithelial cells, such as edema and degeneration, accompanied by a small area of cell necrosis. Sclerosis was found in the glomerulus; Increased positive expression of genes and proteins related to JAK2 and STAT3 (P＜0.05,P＜0.01)was found . Compared to the model group, urantide groups with prolongated medicine administration and the pathological changes that occur in the kidney tissue showed obvious improvement and upturned. At the same time, the degree of glomerulosclerosis decreased and renal tubulointerstitial injury was improved(P＜0.01); Positive expression of genes and proteins related to JAK2 and STAT3 (P＜0.05, P＜0.01) all decreased. Conclusions Urantide may inhibit JAK2/STAT3 signal pathway and this finding may support the development of clinical strategy for the prevention and treatment of AS related renal injury.

Pachymic acid inhibits proliferation of human colon cancer cell line HT-29

FENG Naxin, WANG Peng, HE Mingxuan, LIU Jinyan

2023, 43(11): 1673-1679. doi:10.16352/j.issn.1001-6325.2023.11.1673

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Objective To investigate the impacts of pachymic acid (PA) on proliferation and apoptosis of human colon cancer cell line (HT-29) and its potential mechanism. Methods The cells were divided into blank group, 20, 40 and 80 μmol/L PA groups. The proliferation of HT-29 cells was detected by MTT method; The cell cycle and apoptosis of HT-29 cells were detected by flow cytometry; The expression of cell cycle, apoptosis and Akt/MDM2/p53 signal pathway related proteins in HT-29 cells were detected by Western blot. The tumor xeno-transplantation model with HT-29 cells was constructed, and then the tumorigenic nude mice were randomly divided into control group, intra-peritoneal injection of 10, 30 and 60 mg/kg PA groups(6 in each group), the tumor volume and weight were measured; The expression of Ki-67 and of cleared caspase-3 proteins in tumor tissue was detected by immuno-histochemistry; The expression of Akt/MDM2/p53 signal pathway related protein in tumor tissue was detected by Western blot. Results PA of all doses obviously inhibited proliferation of HT-29 cells, the proportion of cells in S phase and in G₂/M phase was deceased and the expression of B cell lymphoma gene-2(Bcl-2), cyclin D1, cycle protein dependent activating enzyme 2(CDK2) proteins all decreased, The ratios of p-Akt/Akt and p-MDM2/MDM2 in a concentration-dependent manner increased(P＜0.05).The apoptosis rate, the ratio of cells in G₀/G₁ phase and the protein expression of Bax, cleaved caspase-3 and p53 also increased(P＜0.05). PA in all dose groups obviously reduced the tumor volume, weight, the expression of Ki-67 protein and the ratios of p-Akt/Akt, p-MDM2/MDM2 in tumor tissue of nude mice (P＜0.05), and increased the protein expression of cleaved caspase-3 and p53 in tumor tissue(P＜0.05). Conclusions PA can induce apoptosis and cell cycle arrest of colon cancer cells and inhibit cell proliferation. Its mechanism may be related to the regulation of Akt/MDM2/p53 signal pathway.

Methylprednisolone alleviates limb damage in rat models with high-pressure injection injuries of lower limbs

MU Taifei, SHI Yehong, WANG Cheng, CHEN Shijiu

2023, 43(11): 1680-1684. doi:10.16352/j.issn.1001-6325.2023.11.1680

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Objective To study the intervention effect of methylprednisolone(MP) on high pressure injection injuries(HPII) of lower limbs of rats. Methods Thirty rats were randomly divided into control group, model group (high-pressure injection injury model) and treatment group (tail vein injection of MP 30 mg/kg). After 6 hours, the jet area and limb circulation were observed, blood vessels, nerve and muscle tissues were examined by HE staining microscopy. Liver index was calculated and serum TNF-α, IL-1β and IL-6 were tested by ELISA. Comparison between multiple groups was performed by One-way Anova. Results Compared with the control group, limb circulation , blood vessels, nerves and muscles were significantly damaged.The liver index decreased while the serum content of TNF-α, IL-1β, and IL-6 all increased significantly (P＜0.05). Compared with the model group, the above indicators improved significantly in the treatment group(P＜0.05). Conclusions Injection of MP after high-pressure injection injuries can alleviate limb damage, reduce liver load and improve serum inflammatory markers of injured rats.

Screening and verification of key genes for colon cancer based on GEO database

YAN Wen, LI Nan, GU Tongnan, KONG Xiangzhao

2023, 43(11): 1685-1692. doi:10.16352/j.issn.1001-6325.2023.11.1685

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Objective To screen the potential key genes associated with colon cancer(CC) by bioinformatics analysis based on Gene Expression Omnibus (GEO) database, and verify them through in vitro experiments to explore potential colon cancer molecular markers. Methods The human CC data sets GSE10950 and GSE74602 were obtained from GEO database. The differentially expressed genes (DEGs) in colon cancer and adjacent tissues were screened by GEO2R tool and Venn diagram software. DEGs were used to perform by Gene Ontology(GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). The Protein-Protein Interaction (PPI) network of DEGs was constructed by STRING database, then the key genes were screened with Cytoscape software. GEPIA database was used to validate expression and prognostic value of key genes. Finally, the functions of key genes were further verified by cell transfection and CCK8 assays. Results 515 DEGs were obtained from two datasets, of which 223 were up-regulated and 292 were down-regulated. GO enrichment analysis showed that up-regulated DEGs were mainly involved in negative regulation of cell cycle, transcriptional control. KEGG signaling pathways analysis showed that up-regulated DEGs were mainly enriched in cell cycle signaling pathways. A total of 33 hub genes were screened through the PPI. UCLCAN analysis was implemented and 7 of 33 genes were relative to lower survival rate of CC patients. The expression levels of 7 genes by GEPIA analysis (P＜0.05).It was verified by GEPIA database that CCNB1,CCNA2,CDC20,CDKN3,DLGAP5,HMMR and NCAPG were highly expressed in colon cancer tissues. KEGG pathway enrichment and found that three critical genes (CCNB1, CDC20 and CCNA2) enriched in cell cycle pathway. The inhibition of CCNB1,CDC20 or CCNA2 significantly promoted proliferation of colon cancer cells. Conclusions A total of 7 key genes were identified to be involved in the occurrence of CC. The expression of CCNB1,CDC20 and CCNA2 is potentially related to the poor prognosis in CC, which may be potential targets of clinical treatment and prognostic markers for CC patients.

Application of human magnetic induction analyzer in screening gastroduodenal diseases

CHEN Xi, LI Xinlei, XIAO Lyuyao, WANG Jiao

2023, 43(11): 1693-1696. doi:10.16352/j.issn.1001-6325.2023.11.1693

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Objective To study the effectiveness of human magnetic induction analyzer in screening gastric diseases. Methods Totally 112 patients with gastroduodenal diseases admitted to the inpatient department from March 2023 to May 2023 were examined using a human magnetic induction analyzer. After explanation and written consent, the detection effect was evaluated based on the clinical diagnostic results of gastroscopy and/or pathological tissue. Results The detection rates of various gastric diseases by the human magnetic induction analyzer were 88.89% for gastritis, 66.67% for gastric ulcer, 60% for duodenal inflammatory response, and 66.67% for duodenal ulcer respectively. The sensitivity of the human magnetic induction analyzer detection was 88.89%, the specificity was 100.0% and the compliance rate was 88.99%. Conclusions The human magnetic induction analyzer has the advantages of easy and time saving, non-invasive with high safety, sensitivity and consistency. It can be widely used for the preliminary screening of gastroduodenal diseases.

Effect of early rehabilitation exercise on functional rehabilitation and bone mineral density in patients with thoracolumbar compression fracture

BI Fei, QIU Yingying, WANG Yinuo

2023, 43(11): 1697-1701. doi:10.16352/j.issn.1001-6325.2023.11.1697

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Objective To investigate the effect of early rehabilitation exercise combined with percutaneous vertebroplasty on functional rehabilitation and bone mineral density in patients with osteoporotic thoracolumbar compression fractures. Methods From February 2020 to February 2022, 122 patients with osteoporotic thoracolumbar compression fracture in the Department of Rehabilitation Medicine, Beijing Friendship Hospital affiliated to Capital Medical University were randomly divided into the control group (n=61) and the research group (n=61). The T-value of bone mineral density was measured with the EXA-300 dual-energy X-ray bone densitometer. The Cobb angle was measured on the lateral film of the vertebral body. The lumbar function was assessed with the lumbar Japanese Orthopaedic Association Score(JOA) scale and the lumbar Oswestry Dysfunction Index (ODI). The pain was assessed with the Visual Analogue Scale(VAS), and the self-care ability was measured with the Barthel index scale. Results The T value of bone mineral density, Cobb angle,ODI score and VAS score in the research group was lower than those in the control group 1 month and 3 months after the intervention (P＜0.05). The JOA score and Barthel Index were higher than those in the control group(P＜0.05). The incidence of complications in the research group was 3.28% lower than that in the control group 13.11% (P＜0.05). Conclusions Early rehabilitation exercise combined with percutaneous vertebroplasty has a significant effect in the treatment of osteoporotic thoracolumbar compressibility fractures.

Application of improved enhanced recovery after surgery in laparoscopic partial nephrectomy

DONG Dexin, ZHANG Yushi, YAN Weigang, SHI Bingbing

2023, 43(11): 1702-1706. doi:10.16352/j.issn.1001-6325.2023.11.1702

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Objective To investigate the feasibility and safety of modified enhanced recovery after surgery (ERAS) in laparoscopic partial nephrectomy. Methods A retrospective analysis was conducted with the clinical data of renal tumor patients treated with laparoscopic partial nephrectomy in the Urology Department of Peking Union Medical College Hospital from May 2019 to June 2021. According to the peri-operative ERAS strategy, they were divided into modified ERAS group and conventional group. The postoperative recovery and complications of the two groups were compared. Results A total of 243 patients were enrolled, including 142 in the modified ERAS group and 101 in the conventional group. There was no significant difference between the two groups in baseline of indicators such as region, age, gender and tumor stage. The time records of first drinking water, the time of first exhaust, the first off-bed movement, catheter removal and drainage tube removal, the post-operative hospital stay and the total expense of hospitalization in the improved ERAS group were significantly superior to those in the conventional group (all P＜0.05). Conclusions The application of improved ERAS during the peri-operative period of laparoscopic partial nephrectomy may accelerate post-operative recovery.

Research progress of signaling pathways in gastric cancer metastasis

YANG Jiaoyang, SHU Gege, LI Dongbao, ZHOU Jin

2023, 43(11): 1707-1712. doi:10.16352/j.issn.1001-6325.2023.11.1707

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The interaction and mutual regulation of different signaling pathways in cells form a dynamic signal transduction network, which is involved in the development of tumors, as the distant metastasis of gastric cancer is the result of regulation and influence of various signaling pathways. Cancer metastasis is often directly associated with poor prognosis, thus in-depth study of the signaling pathway affecting gastric cancer metastasis is essential strategy to optimize the treatment of advanced gastric cancer patients.

Research progress of perioperative cognitive function changes in patients with ischemic moyamoya disease

CHEN Xuanling, QIN Xuewei, LI Zhengqian, ZHAO Xin, YAO Lan, GUO Xiangyang

2023, 43(11): 1713-1717. doi:10.16352/j.issn.1001-6325.2023.11.1713

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Moyamoya disease (MMD) is a chronic cerebrovascular disease of unknown etiology characterized by progressive stenosis or occlusion of unilateral or bilateral terminal internal carotid arteries, accompanied by the abnormal cerebrovascular network (moyamoya vessels) at the skull base. In recent years, with the continuous improvement of medical technology and research level, the detection rate of peri-operative cognitive changes in MMD patients has gradually increased. This paper reviews the clinical manifestations, pathogenesis and influencing factors of peri-operative cognitive changes in patients with ischemic MMD, and focuses on the electroencephalogram(EEG) and magnetic resonance imaging(MRI) features associated with cognitive impairment in patients with MMD.

Progress on the role of metabolic reprogramming in the occurrence of atrial fibrillation

MIAO Haozhen, TAO Lei, SONG Bingjie, ZHANG Qijun

2023, 43(11): 1718-1722. doi:10.16352/j.issn.1001-6325.2023.11.1718

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Metabolic re-programming is a biological process which promotes cell proliferation by changing the metabolic mode to meet the energy demand. Its occurrence can change the metabolic mode of sugar, amino acid and fatty acid. Atrial fibrillation is one of the common arrhythmia. By regulating the pathway related to metabolic reprogramming may effectively alleviate the symptoms of atrial fibrillation, reduce the susceptibility of atrial fibrillation and improve the quality of life of patients.

Progress on the role of mitophagy in myocardial ischemia-reperfusion injury

MA Congcong, LIU Yang, LYU Yang, WANG Haiping

2023, 43(11): 1723-1727. doi:10.16352/j.issn.1001-6325.2023.11.1723

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Myocardial ischemia-reperfusion injury (MI/RI) is potentially associated with activation of mitophagy through a variety of molecular mechanisms. Moderate mitophagy is effective in maintaining mitochondrial membrane potential and cell membrane structure and function, thereby reducing MI/RI, whereas mitochondrial dysfunction, inadequate clearance or over-activated mitophagy can make MI/RI more severe.

Research progress of endothelial injury in the development of graft-versus-host disease

LU Wenqi, WAN Lin

2023, 43(11): 1728-1732. doi:10.16352/j.issn.1001-6325.2023.11.1728

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Graft-versus-host disease (GVHD) is one of the most serious complications after allogeneic hematopoietic stem cell transplantation (allo-HSCT), resulting a very high mortality risk and no effective diagnosis and treatment method. Although an increasing number of studies suggest that immune-mediated vascular endothelial damage plays an important role in GVHD, the specific mechanism remains elusive. On the basis of endothelial injury caused by radiotherapy and chemotherapy before allo-HSCT and infection, the donor T cells directly contact with the damaged endothelial cell antigen, secrete cytokines and inflammatory mediators to kill endothelial cells, leading to further apoptosis and depletion of endothelial cells, and exacerbating the functional damage of target organs and multi-system injury of the body. To explore the endothelial injury mechanism of GVHD. This review provides a new idea for the prevention and treatment of GVHD.

CALCOCO1 in the progression of malignant tumors

JIA Nan, WANG Zhilian

2023, 43(11): 1733-1737. doi:10.16352/j.issn.1001-6325.2023.11.1733

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Calcium binding and coiled-coil domain 1 (CALCOCO1) is an autophagy regulatory protein being discovered in recent years, which plays an important role in selective autophagy process. The gene coding for autophagy is located at 12q13.13, and its coding protein is CALCOCO1 protein. Which functions by binding to ATG8 family proteins, it anchors to the endoplasmic reticulum and the Golgi apparatus and thus initiate the autophagy of endoplasmic reticulum and Golgi aparatus, and thereby participating in selective autophagy and early stages of autophagosome formation. When the mTOR pathway is activated, mTOR regulates the expression of CALCOCO1 through the autophagy-dependent pathway. CALCOCO1 plays a role in the occurrence and metastasis, and so is a potential target for chemotherapy of malignant tumors.

Application and analysis of multiple teaching modes in histology experimental course

WU Wei, QIU Wenying, QIAN Xiaojing, XU Yuanyuan, CHEN Yongmei

2023, 43(11): 1738-1742. doi:10.16352/j.issn.1001-6325.2023.11.1738

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Objective To carry out offline, online and online-offline blended histology experimental teaching for medical students of different grades, and analyze the effect of various teaching modes. Methods The online-offline hybrid teaching system was constructed through virtual slides website and Moodle platform. The teaching effect was evaluated according to experimental scores and questionnaire feedback. Results The experimental score with online-offline blended teaching mode was significantly higher than that with offline or online teaching modes(P＜0.01).The students were satisfied with the online-offline blended mode (98.21%, 96.43%). Conclusions Application of online-offline blended teaching mode in the histology experimental course could improve the teaching quality and achieve better teaching effects.

Application and evaluation of micro-lecture combined with case-based learning in the teaching of pharmacotherapeutics for undergraduate nursing students

WANG Yucheng, JU Rui, GUO Lei

2023, 43(11): 1743-1749. doi:10.16352/j.issn.1001-6325.2023.11.1743

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Objective To explore the application effect of micro-lecture combined with case-based learning (CBL) in the teaching of pharmacotherapeutics for undergraduate nursing students. Methods Two classes of undergraduate nursing students from School of Nursing, Peking Union Medical College enrolled in 2021 were selected as the research subjects. They were taught by micro-lecture combined with CBL (experimental group) or traditional lecture-based learning (LBL) (control group) respectively; The students' in-class quiz and final exam scores were collected. The questionnaires were distributed to evaluate the teaching effect and learning experience through analyzing the scores and questionnaire results. Results The in-class quiz scores of the experimental group were 77.52±17.68, and those of the control group were 66.34±21.35; The final exam scores of the experimental group were 64.34±26.17, and those of the control group were 52.33±25.55. The scores of the experimental group in both tests were significantly higher than those of the control group, and the differences were statistically significant (P＜0.01). The questionnaire findings indicated that the experimental group improved on the theoretical understanding, knowledge retention, medication practice proficiency, interest in learning, concentration in class, and interaction willingness in comparison to the control group. Conclusions Micro-lecture combined with CBL can enhance the learning effect of pharmacotherapeutics for undergraduate nursing students, and optimize the learning experience. It is worth to be further improved and promoted as a feasible teaching method.

Strengthening and improving report writing during standardized training of residents of ultrasonic medicine

XUE Heng, YE Yuting, HUANG Jiuping, CUI Ligang, JIANG Ling

2023, 43(11): 1750-1753. doi:10.16352/j.issn.1001-6325.2023.11.1750

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The ultrasound diagnosis imaging report reflects the sum of a radiologist's synthesis and judgement into a patient's condition. It is also an important product that is generated by radiologists to help the patients. In spite of the importance of clear and effective report writing, radiologists usually receive little or no formal reporting education. There are key principles when reporting the ultrasound findings, impression, and recommendations to promote report quality and reading experience. The findings should emphasize short and factual observations while avoiding redundancy. The impression is the thoughtful synthesis of findings leading to a diagnosis, a differential diagnosis and management recommendations. Improved report writing is achieved only after persistent learning, follow up and summaries.

Safety management of specialized equipments in medical academic institutes

WANG Dexuan, SHAO Mengli, ZHU Caiyun

2023, 43(11): 1754-1757. doi:10.16352/j.issn.1001-6325.2023.11.1754

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As a common type of equipment used in laboratories of universities and research institutes, especially medical academic institutes, special equipment can lead to safety accidents if used and managed improperly. To address the problems in the institutional framework, personnel management, equipment maintenance, and management methods of special equipment in laboratories, this article proposes a targeted solution based on practical management experience and literature research. This solution not only standardizes the safety management system of special equipment, enhances the efficiency of management departments, but also promotes safety awareness among users, providing a new insight for the safety management of special equipment in academic institutes and effectively preventing safety accidents.

Patient safety indicators in hospital medical quality evaluation system

WU Juan, ZHANG Yun, PENG Hua, ZENG Xuejun

2023, 43(11): 1758-1764. doi:10.16352/j.issn.1001-6325.2023.11.1758

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This review summarizes commonly used hospital-based healthcare quality evaluation systems at home and abroad, and focuses on evaluation indicators regarding patient safety. Through the comparative analysis between different evaluation systems, it tries to shed light on China's healthcare quality evaluation system, especially the indicators related to patient safety. The purpose of this review is to understand and learn from foreign evaluation systems on patient safety, which can help further improve the quality of healthcare in China.

Bibliometrics and visual analysis of the research status of orthopedics in Beijing Jishuitan Hospital

ZHANG Jinqing, LI Cheng, ZHOU Yixin

2023, 43(11): 1765-1770. doi:10.16352/j.issn.1001-6325.2023.11.1765

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Objective To analyze the research status of Beijing Jishuitan Hospital in the field of orthopedics based on bibliometrics. Methods The Web of Science database was used to search the articles published by Beijing Jishuitan Hospital from 2002 to 2021, and the articles related to orthopedics were selected. WPS Office and Bibliometrix software were used for further bibliometric analysis of the data. Results A total of 778 English articles were related to orthopedic research, and the number of articles was increasing year by year. Spinal surgery published the most articles (205 articles). Beijing Jishuitan Hospital completed the most international cooperation with the United States (211 articles), and completed the most domestic cooperation with Beijing research institutions (269 articles). Tian Wei was the author who published the most articles about orthopedics in Beijing Jishuitan Hospital(163 articles). Beijing Jishuitan Hospital had the largest number of orthopedic articles published in CHINESE MEDICAL JOURNAL(69 articles). A total of 481 papers were funded by the fund, most of which were funded by the National Natural Science Foundation of China (266 articles). Conclusions Through bibliometric analysis, the current situation of scientific research in the field of orthopedics in Beijing Jishuitan Hospital is demonstrated, which can provide reference for the formulation of scientific research policies and the development of disciplines.

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