Abstract: Objective To investigate the serum 25-hydroxyvitamin D[25-(OH)D] concentration level of pregnant women in Lhasa, Tibet, and to observe the nutritional status of vitamin D(VD) in different pregnant women. Methods A total of 367 healthy pregnant women in obstetric outpatient clinic of Tibet Autonomous Region People's Hospital from July 2021 to April 2023 were selected as the study subjects, and 209 non-pregnant women of childbearing age who had undergone physical examination during the same period were selected as the normal control group, and the serum 25-(OH)D level was detected by Abbott chem.-iluminescence method, and the statistical analysis was carried out by SPSS statistical software. Results The overall serum level of 25-(OH)D in 367 pregnant women was 11.5(8.5, 17.3)ng/mL. VD adequacy, insufficiency, and deficiency accounted for 16.6%, 30.0%, and 53.4%, respectively. The overall serum level of 25-(OH)D in 209 healthy control women was 9.9(7.7, 13.6)ng/mL. VD adequacy, insufficiency, and deficiency accounted for 6.7%, 27.8%, and 65.5%, respectively. There was no significant difference in serum 25-(OH) D level between 9-13⁺⁶weeks and 15-20⁺⁶weeks pregnant women. Conclusions VD insufficiency or deficiency is not uncommon in pregnant women in Lhasa, and VD supplementation should be given during pregnancy preparation and pregnancy.

Key words: pregnant woman, 25-hydroxyvitamin D, vitamin D