Table of Content

05 January 2026, Volume 46 Issue 1

Previous Issue   

Original Articles

NG2 expression in primary culture of bone marrow derived-macrophages and its bioactivity

CHENG Zhijian, WANG Guoyu, HE Xijing, ZHAI Jingjing, WANG Tiaoning

2026, 46(1):  1-4.  doi:10.16352/j.issn.1001-6325.2026.01.0001

Asbtract ( 33 )   PDF (2033KB) ( 11 )  

References | Related Articles | Metrics

Objective To study the neuron-glial antigen 2(NG2)-positive macrophages and their bioactivity in primary cultured mouse bone marrow derived macrophages. Methods Bone marrow single cell suspension was isolated from femur of 6-8 weeks old Kunming mouse co-cultured with L929 supernatant. NG2 expression was detected by immuno-fluorescence staining microscopy at 1, 3, 5 and 7 days. The proliferation and phagocytosis of NG2-positive macrophages were examined by immunofluorescence staining microscopy and EdU labeling. Results The expression of NG2 markers and positive rate of EdU in NG2-positive macrophages all increased gradually with the prolongation of culture time(P＜0.05). Conclusions NG2-positive macrophages from mouse bone marrow show significant proliferation and phagocytosis function.

Tamoxifen citrate promotes proliferation of Leydig cell line R2C after treatment with TGF-β1

WANG Yeting, WU Peng, BIAN Yanchao, WEN Jianxun, NIU Lili

2026, 46(1):  5-10.  doi:10.16352/j.issn.1001-6325.2026.01.0005

Asbtract ( 20 )   PDF (4038KB) ( 9 )  

References | Related Articles | Metrics

Objective To investigate the effect of tamoxifen citrate (TCT) on Leydig cell line R2C treated by TGF-β1. Methods R2C cells were used as the research subjects. CCK-8 test was used to detect the effect of different concentration gradients of TCT on the proliferation activity of normal R2C cells to fix on the most effective concentration. TGF-β1 was incubated with R2C cells for modeling, and then TCT was added into the medium at a concentration that significantly promoted R2C cell proliferation. R2C cell proliferation activity, basal testosterone, estradiol and cell cycle were detected. Results TGF-β1 treatment with 4 ng/mL, 5 ng/mL and 6 ng/mL TCT for 24, 48, and 72 hours significantly promoted R2C cell proliferation (P＜0.05); ELISA showed that treatment with 5 ng/mL and 6 ng/mL TCT for 48 hours resulted in a decrease in basal level of estradiol(E2)(P＜0.05) and an increase in basal level of testosterone(T) (P＜0.05). Flow cytometry showed a significant increase cell counting in G1 phase in the 6 ng/mL TCT treatment group, and the cell cycle arrested in the G1 phase. Conclusions A certain dose of TCT can improve the negative hormone level changes caused by exogenous injury of Leydig cell line R2C and promote its proliferation.

Hypoxia causes damage to primary cultured human bronchial epithelial cells

HUANG Jianmin, HE Shan, ZHANG Qiuyun, LIU Yunliang, YOU Yongjing

2026, 46(1):  11-15.  doi:10.16352/j.issn.1001-6325.2026.01.0011

Asbtract ( 20 )   PDF (2794KB) ( 7 )  

References | Related Articles | Metrics

Objective To explore the tissue damage and underlying mechanism of hypoxia on human bronchial epithelial cells. Methods Human primary cultured bronchial epithelial cells were divided into three groups: 1)Control group: conventionally cultured in normal environment for 6 hours; 2)Hypoxia group: cultured in 5% O₂ environment for 6 hours; 3)Fumigation group: cultured in 5% cigarette smoke environment for 6 hours. Transmission electron microscopy was used to observe the morphological changes of organelles. Trans-epithelial cell electrical resistance detection was used to evaluate cell permeability, apoptosis was detected by flow cytometry and the expression of atresia band protein 1 (ZO-1) and transmembrane proteins (occludin and claudin-1) was detected by Western blot. Results 1)Compared with control group, the apoptosis rate of cells in the hypoxia group and fumigation group increased while cell resistance decreased and the intercellular permeability increased. 2)The expression of claudin-1 protein decreased in the hypoxia group, the expression of occludin,claudin-1 and ZO-1 proteins in the fumigation group decreased significantly. 3)The nuclear membranes of cells in the hypoxia group and fumigation group shranked and mitochondria were damaged. The autophagosomes and apoptotic bodies were found in cell cytoplasm. Conclusions Hypoxia down-regulates the expression level of tight junction proteins in human bronchial epithelial cells and increases cell permeability, which may lead to impaired epithelial barrier function by demolishing tight junction structures.

Level of serum galectin-10,STAT3 and CCL19 is correlated with disease severity in patients with chronic eczema

MENG Xiaohui, JIANG Shiyao, YANG Yi

2026, 46(1):  16-21.  doi:10.16352/j.issn.1001-6325.2026.01.0016

Asbtract ( 22 )   PDF (1159KB) ( 4 )  

References | Related Articles | Metrics

Objective To investigate potential correlation of serum galectin-10(Gal-10), signal transducer, activator of transcription 3 (STAT3) and C-C chemokine ligand 19 (CCL19) with severity of chronic eczema. Methods TFrom October 2022 to October 2024,104 patients admitted to the 7th People's Hospital of Zhengzhou with chronic eczema were reviewed. Based on implicated area of eczema and severity index (EASI)score, they were assigned into mild group (EASI score≤10 points,43 cases),moderate group (10 points＜EASI score ≤ 19 points,36 cases),and severe group (EASI score ≥ 20 points,25 cases). Meantime, 60 healthy individuals from routine physical check were selected as control group. ELISA was used to detect serum Gal-10,STAT3,and CCL19. Spearman correlation was performed to analyze the correlation between serum Gal-10,STAT3,CCL19 levels with EASI score in patients. Logistic regression was performed to analyze the factors affecting the condition of patients. Receiver operating characteristic (ROC) curves were used to evaluate the diagnostic value of serum Gal-10,STAT3,and CCL19 for the severity of chronic eczema. Results The case group had manifestly higher serum Gal-10,STAT3 and CCL19 than control group (P＜0.05). The moderate and severe groups had conspicuous higher EASI scores and serum Gal-10,STAT3 and CCL19 than mild group (P＜0.05); And with severe group being conspicuously higher than moderate group (P＜0.05). Serum Gal-10,STAT3,CCL19 in patients with chronic eczema were positively correlated with EASI score (r=0.606,0.417,0.507,P＜0.05). The elevated EASI score, serum Gal-10,STAT3 and CCL19 were all believed to be risk factors to aggravate chronic eczema (P＜0.05). The area under the curve (AUC) of serum Gal-10,STAT3,CCL19 alone and in combination for diagnosing the severity of chronic eczema was 0.827,0.811,0.803,and 0.914,respectively. The combined diagnostic efficacy was superior to the individual detection of the three (Z_{three combination-Gal-10}=2.703, Z_{three combination-STAT3}=2.251,Z_{three combination-CCL19}=2.795,all P＜0.05). Conclusions The serum level of Gal-10, STAT3 and CCL19 in patients with chronic eczema are elevated and correlated with severity of the disease. The combined detection of the three combined index factor may support clinical evaluation of the severity of chronic eczema.

Level of serum CHI3L1 and sFlt-1 is correlated with disease severity and prognosis of patients with IgA nephropathy

SUN Yingchun, LI Jianjun, YANG Zaibo, YAN Junhui, LIU Yushuo

2026, 46(1):  22-27.  doi:10.16352/j.issn.1001-6325.2026.01.0022

Asbtract ( 15 )   PDF (1075KB) ( 2 )  

References | Related Articles | Metrics

Objective To investigate the relationship of the serum chitinase-3-like protein 1 (CHI3L1) and soluble fms-like tyrosine kinase-1(sFlt-1) with the severity and prognosis of IgA nephropathy patients. Methods One hundred and twenty-four patients with IgA nephropathy in Third Hospital of Peking University from September 2022 to August 2024.The cases were divided into mild, moderate and severe groups according to the severity of the disease.The prognosis was classified as good and poor prognosis groups; Serum CHI3L1 and sFlt-1 were measured by ELISA. The influencing factors of poor prognosis in IgA nephropathy patients were analyzed using logistic regression. ROC curve was plotted to analyze the diagnostic value of serum CHI3L1 and sFlt-1 in patients with severe IgA nephropathy and their predictive value for poor prognosis in IgA nephropathy patients. Results The level of serum CHI3L1,sFlt-1,Scr,BUN,24-hour urinary protein and UA in the moderate and severe groups was found to be significantly higher than that in the mild group(P＜0.05),while Hb,Alb,and eGFR were prominently lower than those in the mild group(P＜0.05).Additionally, the level of CHI3L1,sFlt-1,Scr,BUN,24-hour urinary protein and UA in the severe group were significantly higher than those in the moderate group(P＜0.05),Hb,Alb,and eGFR were obviously lower than those in the moderate group(P＜0.05). According to the ROC curve, the AUC of serum CHI3L1 and sFlt-1 in the diagnosis of severe IgA nephropathy patients was 0.833 and 0.865 respectively. The AUC of the combination of the two in the diagnosis of severe IgA nephropathy patients was 0.970,and the combination of the two was better than alone in diagnosis(Z=2.712,Z=2.725,both P＜0.05). Scr,BUN,24 h urine protein quantification and UA were significantly higher in the poor prognosis group than those in the good prognosis group(P＜0.05)and Hb,Alb and eGFR were significantly lower than in the good prognosis group(P＜0.05). Compared with the good prognosis group, the level of serum CHI3L1 and sFlt-1 were prominently higher in the poor prognosis group(P＜0.05). Multivariate logistic regression analysis revealed that CHI3L1,sFlt-1,Scr and 24-hour urinary protein quantification were risk factors predicting poor prognosis(P＜0.05) while eGFR was a protective factor(P＜0.05). According to the ROC curve, the AUC of serum CHI3L1 and sFlt-1 in predicting poor prognosis in IgA nephropathy patients was 0.820 and 0.771, respectively. The AUC of the combination of the two in predicting poor prognosis in IgA nephropathy patients was 0.879,and the combination of the two was better than single prediction(Z=2.692,Z=2.699,both P＜0.05). Conclusions The expression level of serum CHI3L1 and sFlt-1 is significantly elevated in patients with IgA nephropathy, and related to the severity and prognosis of the disease. Combined detection has certain clinical value in predicting the prognosis of patients.

PM2.5 in the air exacerbates lung injury in a mouse model of chronic obstructive pulmonary disease

NI Xing, LIU Fengchun, LU Lin, MAO Shaohua, BI Jirui, ZHANG Run

2026, 46(1):  28-32.  doi:10.16352/j.issn.1001-6325.2026.01.0028

Asbtract ( 17 )   PDF (2818KB) ( 3 )  

References | Related Articles | Metrics

Objective To investigate the exacerbating effects of fine particulate matter (PM2.5) on lung injury in a chronic obstructive pulmonary disease (COPD) mouse model, and to provide a reliable experimental basis for further research. Methods Mice were randomly divided into four groups: control group, COPD group induced by cigarette smoke and lipopolysaccharide (LPS),KP group mice induced by intranasal instillation of Klebsiella pneumonia(KP),and PM2.5 group (COPD model) induced by intranasal instillation of PM2.5). Lung function test was used to check forced vital capacity (FVC), inspiratory capacity (IC), forced expiratory volume in 0.1 seconds (FEV0.1), FEV_0.2,FEV_0.1/FVC ratio and FEV_0.2/FVC ratio. Arterial blood gas analysis was applied to measure partial pressure of oxygen (PaO₂), partial pressure of carbon dioxide (PaCO₂), pH, and oxygen saturation (SaO₂). Lung histopathology was evaluated via hematoxylin-eosin (HE) staining microscopy. Results Compared with the control group,COPD,KP and PM2.5 groups showed significantly increased FVC,IC,FEV_0.1,FEV_0.2 and PaCO₂ (P＜0.05),along with significantly decreased FEV_0.1/FVC,FEV_0.2/FVC,PaO₂ and SaO₂ (P＜0.05). Histopathology microscopy showed typical inflammatory responses and alveolar structural damage. Compared with the COPD group,KP and PM2.5 groups exhibited further increases in FVC,FEV_0.1,and FEV_0.2(P＜0.05),reduced FEV_0.1/FVC and FEV_0.2/FVC ratios, elevated IC and PaCO₂ (PM2.5 group only,P＜0.05),and decreased SaO₂ (PM2.5 group only,P＜0.05). Histopathology microscopy confirmed aggravated inflammatory responses and alveolar destruction in these groups. Conclusions PM2.5 exacerbates pulmonary dysfunction and lung injury in COPD mice.

Proteomics of glioblastoma based on microdissection

NI Yanying, ZHAI Chunyan, JIANG Zhongcai, YUAN Yuan

2026, 46(1):  33-38.  doi:10.16352/j.issn.1001-6325.2026.01.0033

Asbtract ( 20 )   PDF (4847KB) ( 5 )  

References | Related Articles | Metrics

Objective To analyze differentially expressed proteins in glioblastoma(GBM) tissues and to provide potential clews for early diagnosis of glioblastoma. Methods A total of 27 formalin fixed paraffin embedded(FFPE) specimens of glioblastoma tumor tissues,glioblastoma paired para-neoplastic tissues and non-tumor brain tissues were collected. The tissue samples were obtained using laser capture microdissection(LCM),followed by liquid chromatography-mass spectrometry for quantitative proteomic analysis. The screened differentially expressed proteins were subjected to functional analysis and three of them were selected for immunohistochemical validation. Results A total of 6 205 proteins were identified in these tissue samples. Compared with the non-tumor group,142 proteins were significantly expressed abnormally in the paraneoplastic group and 1 273 proteins in the tumor group; Compared with the paraneoplastic group,743 proteins were significantly expressed abnormally in the tumor group. PTBP1,AK2 and SHMT2 were studied and immunohistochemically verified, the results showed that all three proteins were significantly up-regulated in the tumor group(P＜0.05) as compared with the non-tumor group and paraneoplastic group. Conclusions Significant proteomic differences between tumor,para-neoplastic and non-tumor tissues of glioblastoma provide important clues for in-depth study of early diagnosis of glioblastoma.

Berberine enhances the chemotherapy sensitivity of prostate cancer cell line 22RV1 to docetaxel

LU Wei, WANG Yanmin, LIU Bingchen, TAO Xin, WANG Shipeng, CAO Qiuye

2026, 46(1):  39-45.  doi:10.16352/j.issn.1001-6325.2026.01.0039

Asbtract ( 15 )   PDF (4303KB) ( 3 )  

References | Related Articles | Metrics

Objective To investigate the chemotherapy effectiveness of berberine (BBR) to enhance sensitivity of prostate cancer cell line 22RV1 to docetaxel. Methods The docetaxel (DTX) resistant cell was constructed using prostate cancer cell line 22RV1. The drug-resistant cell strain(22RV1/DTX) was divided into: 22RV1/DTX group, low concentration (L)-BBB+22RV1/DTX group, high concentration (H)-BBB+22RV1/DTX group and H-BBR+ERK activator+22RV1/DTX group. CCK8 method was applied to detect cell proliferation. Scratch healing test was applied to detect migration. Transwell test was applied to detect invasion. Flow cytometry was applied to detect apoptosis. Western blot was applied to detect the expression of extra-cellular regulated protein kinases (ERK), pERK, and ETS transcription factor (ELK1) proteins in cells. Results Compared with 22RV1/DTX group, the proliferation, migration, and invasion of cells in the L-BBR+22RV1/DTX group and H-BBR+22RV1/DTX group were lower but the apoptosis rate was higher (P＜0.05). The proliferation, migration, and invasion of cells in the H-BBR+ERK activator+22RV1/DTX group were higher, and the apoptosis rate was lower (P＜0.05). BBR significantly reduced the pERK/ERK ratio and ELK1 protein expression level in 22RV1/DTX cells(P＜0.05).The effects on 22RV1/DTX cells and pathways were reversed by ERK activator (P＜0.05). Conclusions BBR may inhibit the proliferation, migration, and invasion of drug-resistant cells, promote cell apoptosis and thus enhance the chemotherapy sensitivity of prostate cancer cells to DTX with underlying mechanism of suppressing the ERK/ELK1 signaling pathway.

Endovascular transplantation of endothelial progenitor cells co-infected with exogenous Ang-1 and miR-210 promotes angiogenesis in diabetic chronic lower limb ischemia

LI Chunmeng, SUN Huiyan, ZHENG Xiangjian, XIE Shangshang, LIN Deyong, LIU Zitian

2026, 46(1):  46-55.  doi:10.16352/j.issn.1001-6325.2026.01.0046

Asbtract ( 18 )   PDF (6070KB) ( 3 )  

References | Related Articles | Metrics

Objective To investigate the co-modification of endothelial progenitor cells(EPCs) by angiopoietin-1 (Ang-1) and microRNA-210 (miR-210). The anti-apoptosis, migration and angiogenesis promotion of endothelial progenitor cells (EPCs) provide experimental basis for the treatment of diabetic chronic lower limb ischemia. Methods EPCs in bone marrow of SD rats were isolated and phenotype was identified by Dil-ac-LDL/FITC-UEA-1 double fluorescence staining microscopy and by flow cytometry (for checking CD34⁺/CD133⁺). Ang-1 and miR-210 over-expressed lentiviral vectors were constructed and infected into EPCs, gene expression was detected by RT-qPCR. Annexin V/PI staining, Transwell assay and ECMatrix gel angiogenesis assay were used to evaluate cell apoptosis rate, migration and tube formation in medium with high glucose and low oxygen (22.0 mmol/L glucose, 3% O₂) conditions. The rat hind limb ischemia model was established; And 28 days after intracavity transplantation modified EPCs; Micro-vascular density was detected by CD31 immuno-histochemistry; And Ang-1/Tie2/PI3K/AKT path-related protein expression was analyzed by Western blot. Results EPCs was successfully cultured and identified. The expression of Ang-1 and miR-210 in the co-transfection group was 3.88 times and 4.21 times higher than those in the control group respectively(P＜0.01). The EPCs apoptosis rate (10.84%) in the co-transfection group was significantly lower than that in the control group (26.22%, P＜0.01). The counting of migrating cells (78.3±5.2) was increased by 3.64 times (P＜0.01). The quantity of vascular branches (15.6±1.8) was increased by 3.7 times (P＜0.01). The microvascular density (97.7±12.5) in the cotransfection group was 3.71 times higher than that in the control group (26.3±18.4,P＜0.05) and the expression of Ang-1, VEGF, P-Akt and P-VEGFR in ischemic tissue was significantly up-regulated(P＜0.05). Conclusions The co-modification of Ang-1 and miR-210 can significantly improve the survival of EPCs in diabetic ischemic micro-environment and promote angiogenesis. Its mechanism is closely related to the activation of Ang-1/Tie2/PI3K/AKT pathway.

Grifola frondosa polysaccharide improves dexamethasone induced atrophy of myotube models from mouse skeletal muscle cell line C2C12

ZHANG Zhaobo, ZHANG Wenling, ZHAO Feifei, DU Guotao

2026, 46(1):  56-62.  doi:10.16352/j.issn.1001-6325.2026.01.0056

Asbtract ( 14 )   PDF (4146KB) ( 2 )  

References | Related Articles | Metrics

Objective To investigate the mechanism by which Grifola frondosa polysaccharide (GFP) mitigates dexamethasone (DEX)-induced atrophy of myotubes in mouse skeletal muscle cell line C2C12(C2C12 myotube). Methods C2C12 cells were divided into normal group (NOR), dexamethasone group (DEX), low-dose GFP (GFP-L), medium-dose GFP (GFP-M) and high-dose GFP-H intervention DEX groups (concentrations of 20, 50, and 100 μg/mL, respectively).Immunofluorescence microscopy and Western blot were employed to assess myosin heavy chain (MyHC) expression. Western blot and RT-qPCR were utilized to evaluate the protein and mRNA levels of muscle-specific ring finger protein 1 (MuRF1), muscle atrophy F-box protein (Atrogin1) as well as phosphorylation of AMPK and forkhead box protein O3 (FOXO3a).The expression of SIRT1.ATP and mitochon- drial protein content were measured with commercially available kits. Results GFP-M and GFP-H groups significantly increased myotube length, fusion index and MyHC expression in DEX-treated cells(P＜0.05). Additionally, GFP treatment markedly down-regulated the protein and mRNA expression level of Atrogin1, MuRF1 and Myostatin(P＜0.05). The phosphorylation level of AMPK and FOXO3a and the expression of SIRT1 were also significantly reduced(P＜0.05).The SIRT1 inhibitor-induced aberrant expression of FOXO3a, Atrogin1, and MuRF1 was effectively reversed by GFP. Conclusions Grifola frondosa polysaccharide may alleviate DEX-induced atrophy of C2C12 myotubes through a SIRT1-mediated AMPK/FOXO3a signaling pathway.

Characteristics of fecal metabolomics about therapeutic effects of statins for coronary heart disease

LI Ruoning, XU Liang, ZHAO Yingchun, SHAO Jiqing, JU Bowei

2026, 46(1):  63-70.  doi:10.16352/j.issn.1001-6325.2026.01.0063

Asbtract ( 18 )   PDF (5600KB) ( 2 )  

References | Related Articles | Metrics

Objective To explore the relationship between fecal metabolomics characteristics and the therapeutic effects of statins. Methods Fecal samples were collected from 60 patients with coronary heart disease undergoing statin therapy. Based on whether their low-density lipoprotein (LDL) level were below 1.9 mmol/L after treatment, the patients were divided into an effective treatment group and ineffective treatment group with 30 cases in each. Non-targeted metabolomics and targeted short-chain fatty acid sequencing were performed with the fecal samples and the sequencing results were analyzed. Results Principal Component Analysis (PCA) showed significant differences between the effective and ineffective treatment groups. Differential analysis identified 319 ions significantly different between the two groups in positive ion mode and negative ion mode. Enrichment analysis indicated that several pathways including taurine and hypotaurine metabolism, riboflavin metabolism, and fatty acid metabolism were enriched. Based on 113 diagnostic models, the top 10 models with the best predictive ability for treatment efficacy were selected, with Prilocaine, Glufosinate, and His-Gly-His showing the highest predictive capability across all models [area under the curve(AUC＞0.6)]. The level of Prilocaine and His-Gly-His was higher in effective treatment group while the level of Glufosinate was lower in the effective treatment group (P＜0.05). Additionally, short-chain fatty acid sequencing results showed that the level of Butyric acid and Propionic acid were significantly lower in the effective treatment group (P＜0.01). Butyric acid and Propionic acid were negatively correlated with Prilocaine and positively correlated with His-Gly-His. Conclusions The association between the efficacy of statins therapy and fecal metabolomics characteristics highlights the predictive value of changes in metabolite level such as Prilocaine, Glufosinate, and His-Gly-His which are potentially regulated by short-chain fatty acids.

Polyene phosphatidylcholine repairs high glucose-induced human umbilical vein endothelial cells damage

CHEN Xing, LI Yulong, ZHANG Bing, LIANG Jinjie, CHEN Ziyi

2026, 46(1):  71-77.  doi:10.16352/j.issn.1001-6325.2026.01.0071

Asbtract ( 17 )   PDF (4357KB) ( 4 )  

References | Related Articles | Metrics

Objective Exploring whether polyene phosphatidylcholine (PPC) has a reparative effect on high glucose-induced functional damage of human umbilical vein endothelial cells (HUVECs). Methods MTT assay was used to detect the effect of PPC on the proliferation activity of HUVECs damaged by high concentration of glucose. Cell scratch assay and Transwell migration assay were used to investigate the effect of PPC on the migration ability of high glucose damaged HUVECs. The angiogenesis experiment was conducted to investigate the effect of PPC on the ability of high concentration glucose-induced HUVECs to differentiate into blood vessels. Western blot was used to detect the effect of PPC on the expression of Bax, Bcl-2, caspase-3, and VEGFA proteins in HUVECs damaged by high concentration of glucose. Results PPC significantly restored the inhibitory effect of glucose on HUVECs proliferation and significantly promoted the recovery of high glucose-damaged HUVECs migration ability. PPC also significantly promoted the recovery of the ability of high glucose-damaged HUVECs to differentiate and form blood vessels. Western blot results showed that PPC inhibited the expression of proapoptotic proteins Bax and caspase-3 in HUVECs tissue. Conclusions PPC promotes the proliferation, migration, and vascular differentiation of high glucose-induced HUVECs by increasing VEGFA expression and inhibits apoptosis of HUVECs damaged by high concentration of glucose through Bax/Bcl-2/caspase-3 signaling pathway.

Quercetin inhibits TNF-α-induced inflammation of mouse aortic vascular smooth muscle cell line MOVAS

QU Gairu, XI Shibing, ZHANG Zongli, LI Tao

2026, 46(1):  78-84.  doi:10.16352/j.issn.1001-6325.2026.01.0078

Asbtract ( 18 )   PDF (2697KB) ( 3 )  

References | Related Articles | Metrics

Objective To explore the effect of quercetin(Que)on TNF-α-induced inflammation of vascular smooth muscle cells (VSMCs) and potential mechanism. Methods Mouse aortic vascular smooth cell line MOVAS was exposed to different concentrations of quercetin and the cytotoxicity of quercetin was detected by CCK8 method; TNF-α was used to induce inflammation of MOVAS cells from patients with Kawasaki disease(KO). Cells were exposed to quercetin and NF-κB p65 inhibitors, mRNA and protein expression of inflammatory factors VCAM-1, ICAM-1, MCP-1, IL-1β, IL-6, channel protein NF-κB p65 and phosphorylated NF-κB p65 were detected by RT-qPCR and Western blot; BCECF-AM fluorescent probe was used to detect the adhesion ability of monocytes (THP-1) to MOVAS cells; Immunofluorescence was used to detect NF-κB p65 nuclear translocation. TNF-α significantly induced inflammation of MOVAS cells with expression of inflammatory factors VCAM-1, ICAM-1, MCP-1, IL-1β and IL-6 (P＜0.05). THP-1 cells adhesion was significantly increased (P＜0.001). However, quercetin significantly reduced the expression of the above inflammatory factors and THP-1 cells adhesion (P＜0.05).Quercetin significantly down-regulated phosphorylation (P＜0.01) and nuclear translocation levels of NF-κB p65 subunit induced by TNF-α in MOVAS cells as compared to the control group. Conclusions Quercetin potentially plays a beneficial role in Kawasaki disease by inhibiting the activation of the NF-κB signaling pathway to ameliorate TNF-α-induced inflammation of vascular smooth muscle cells.

Heme oxygenase-1 mediates sodium butyrate to alleviate non-alcoholic steatohepatitis

WANG Aojun, YAO Ruixin, LYU Yue, XU Yu, ZHANG Xiaotian

2026, 46(1):  85-91.  doi:10.16352/j.issn.1001-6325.2026.01.0085

Asbtract ( 15 )   PDF (3495KB) ( 2 )  

References | Related Articles | Metrics

Objective To explore the mechanism of heme oxygenase-1(HO-1) in the alleviation of non-alcoholic steatohepatitis (NASH) by sodium butyrate (SB). Methods 1)Mice were randomly divided into a control group, a high-fat high-cholesterol group(HF group) and a high-fat high-cholesterol+natriumbutyrate group (HF+SB group). The total cholesterol (TC), triglycerides(TG), alanine aminotransferase(ALT), aspartate aminotransferase(AST), and tumor necrosis factor(TNF-α) in serum of each group were measured using colorimetric methods. Mice livers were isolated and morphological changes were observed with microscopy by HE and Masson staining. The expression of TNF-α, collagen(COL), and HO-1 in the liver tissues were detected by Western blot; 2)HepG2 cells were divided into a control group, a control+sodium butyrate group (control+SB group), a model group(model group), and a model+sodium butyrate group (model+SB group), and the expression of the aforementioned indicators was measured in each group. Results 1)SB intervention significantly reduced the body weight, liver weight, and liver weight index of NASH mice(P＜0.05),decreased the level of TC, TG, ALT, AST, and TNF-α in serum as well as the expression of TNF-α and collagen(COL) proteins in the liver of NASH mice(P＜0.05). At the same time, SB intervention significantly increased the expression of HO-1 protein in the liver tissue of NASH mice(P＜0.05);2)SB intervention significantly reduced the level of TC, TG, ALT and AST in group model cells and increased HO-1 protein expression in group model cells (all P＜0.05). Conclusions HO-1 plays a crucial role in the alleviation of NASH by sodium butgrate, potentially through mechanisms related to the suppression of hepatic inflammatory and oxidative stress responses.

Screening of soluble hemagglutinin recombinant proteins for influenza virus B/Victoria

WANG Jingfan, YANG Jiaojiao, ZHANG Ting, WANG Zhirong, XU Xuemei

2026, 46(1):  92-96.  doi:10.16352/j.issn.1001-6325.2026.01.0092

Asbtract ( 16 )   PDF (1836KB) ( 4 )  

References | Related Articles | Metrics

Objective To screen the soluble hemagglutinin (HA) recombinant protein derived from B/Victoria lineages of influenza virus that possess hemagglutination activity. Methods Based on the B/Darwin/7/2019 (B/Victoria) strain, HA mutant genes BV-T1, BV-T2, and BV-T3 were obtained by fusing the leucine zipper GCN4pII and GCN4pLL trimerization motifs(T1 and T2), and bacteriophage T4 Foldon trimerization motifs(T3) to the C-terminus of the HA ectodomain. HA ectodomain mutant gene BV-ecto was also constructed as a control. These genes were inserted into pFastBac1 vector. The proteins were expressed using baculovirus-Sf9 insect cell expression system and culture supernatants of infected cells were harvested. Recombinant proteins were purified by Strep-Tactin affinity chromatography and subsequently characterized for their oligomerization degree and hemagglutination activity. Results All four mutant proteins were solubly expressed in the culture supernatant. BV-T2 and BV-T3 exhibited a high trimerization form, while BV-T1 formed predominantly trimers with minor low-order oligomers. BV-ecto existed as a monomer.BV-T1 and BV-T2 exhibited hemagglutination activity, in contrast, BV-T3 and BV-ecto lacked hemagglutination activity. Conclusions The soluble BV-T2 mutant protein present efficient trimer- ization and possesses hemagglutination activity.The leucine zipper trimerization motif GCN4pLL is suitable for the soluble expression of B/Victoria lineage HA recombinant protein and thus provides a reference for the development of soluble recombinant protein vaccines against B/Victoria virus.

Detection of bone mineral density combined with serum 25-(OH)D₃ and ucOC assists in the diagnosis of T2DN complicated with sarcopenia

RUAN Zheng, GE Xin, LIAO Yanyuan, ZHANG Shuya

2026, 46(1):  97-102.  doi:10.16352/j.issn.1001-6325.2026.01.0097

Asbtract ( 13 )   PDF (1118KB) ( 2 )  

References | Related Articles | Metrics

Objective To explore the clinical significance of bone mineral density (BMD),serum 25 hydroxyvita-min D₃[25-(OH)D₃],uncarboxylated osteocalcin (ucOC) and sarcopenia in patients with type 2 diabetes nephro- pathy(T2DN). Methods From August 2023 to August 2024,197 patients with T2DN admitted to the department of Endocrinology, People's Hospital of Wuhan University were assigned into sarcopenia group (80 cases) and non-sarcopenia group (117 cases) according to whether the patients had sarcopenia or not. ELISA reagent test kit was applied to detect the levels of serum 25-(OH)D₃ and ucOC. Dual energy X-ray absorptiometry (DXA) was applied to detect the BMD value of the patients. The Modified Quantitative Subjective Global Assessment(MQSGA) was applied to evaluate the nutritional status of patients. The correlation between BMD value, 25-(OH)D₃, ucOC and sarcopenia index and MQSGA score was analyzed by Pearson and Spearman methods. Multivariate Logistic regression model was used to analyze the factors affecting sarcopenia in T2DN patients. Receiver operating characteristic (ROC) curve was used to analyze the auxiliary diagnostic value of BMD value, serum 25-(OH)D₃ and ucOC for sarcopenia in T2DN patients. Results Value and serum 25-(OH)D₃ level in sarcopenia group were lower than those in non-sarcopenia group, and serum ucOC level was higher than those in non sarcopenia group (t/P=9.963/＜0.001,8.948/＜0.001,9.913/＜0.001). Compared to the non-sarcopenia group, participants in the sarcopenia group exhibited significant reductions in grip strength, gait velocity, and ASMI,but sitting time and MQSGA score were higher than those in the non-sarcopenia group (t/P=8.330/＜ 0.001,15.198/＜0.001,8.230/＜0.001,6.934/＜0.001,3.528/0.001). In sarcopenic 2DN patients,BMD and serum 25-(OH)D₃ levels demonstrated positive correlations with grip strength, walking speed and ASMI,while showing inverse associations with sitting time and MQSGA score [BMD value: r_s/P=0.492/＜0.001,0.469/＜0.001,0.492/＜0.001,-0.513/＜0.001,-0.523/＜0.001; 25-(OH)D₃: r_s/P=0.537/＜0.001,0.472/＜0.001,0.496/＜0.001,-0.562/＜0.001,-0.523/＜0.001]. Serum ucOC concentration demonstrated inverse correlations with grip strength, walking speed, and ASMI,while showing positive associations with sitting time and MQSGA score (r_s/P=-0.546/＜0.001,-0.493/＜0.001,-0.487/＜0.001,0.541/＜0.001,0.544/＜0.001). Elevated serum ucOC level is an independent risk factor for sarcopenia in T2DN patients [OR(95% CI)=1.953(1.279-2.982)]. BMD and elevated serum 25-(OH)D₃ levels were independent protective factor[OR(95% CI)=0.316(0.135-0.741),0.287(0.133-0.621)]. The area under the curve (AUC) of BMD,serum 25-(OH)D₃ and ucOC levels in T2DN patients with sarcopenia were 0.838,0.817,0.863 and 0.956,respectively,and the AUC of the combination of the three(triple detection) was greater than that of the single diagnosis (Z/P=4.549/＜0.001,5.109/＜0.001,4.010/0.001). Conclusions In addition to BMD value, serum 25-(OH)D₃ and ucOC are also important biomarkers in patients with T2DN. Triple detection can support to diagnose sarcopenia in T2DN patients.

PHLPP2 variation is associated with prognosis as well as ferroptosis in patients of advanced lung adenocarcinoma harboring EGFR+TP53 co-mutations

ZHANG Yufeng, ZHANG Xinna, ZHOU Yanjiao

2026, 46(1):  103-108.  doi:10.16352/j.issn.1001-6325.2026.01.0103

Asbtract ( 13 )   PDF (1949KB) ( 2 )  

References | Related Articles | Metrics

Objective To investigate the relationship between the PHLPP2 and the efficacy of targeted combined chemotherapy as well as ferroptosis in patients with advanced lung adenocarcinoma (LUAD) harboring EGFR+TP53 co-mutations. Methods Data of 98 patients with advanced LUAD with EGFR+TP53 co-mutation treated with targeted combination chemotherapy from August 2018 to August 2023 were reviewed. The PHLPP2 variations were analyzed and divided into poor prognosis group (n=47) and good prognosis group (n=51) according to prognosis. The dose-response relationship between ferroptosis indicators and poor prognosis was analyzed by restricted cubic spline (RCS) model. The levels of ferroptosis indicators in different PHLPP2 mutations and their relationship with poor prognosis were compared. Results PHLPP2 mutation rate was 30.61%, mainly missense mutation and point mutation. Serum ferritin (SF), malondialdehyde (MDA) and reactive oxygen species (ROS) in poor prognosis group were lower than those in good prognosis group (P＜0.05), and glutathione peroxidase 4 (GPX4), glutathione (GSH) and PHLPP2 mutants in poor prognosis group were higher than those in good prognosis group(P＜0.05). The risk of poor prognosis was correlated with SF, GPX4, GSH, MDA and ROS in a nonlinear dose-response relationship (P_{for non linear}＜0.05). SF, MDA and ROS of wild type were higher than those of mutant type (P＜0.05), GPX4 and GSH were lower than those of mutant type (P＜0.05). There were differences in poor prognosis between different PHLPP2 and different ferroptosis indicators (P＜0.05). Conclusions PHLPP2 mutation affects the clinical efficacy of targeted combination chemotherapy in the treatment of advanced LUAD with EGFR+TP53 co-mutation, and is associated with ferroptosis.

Clinical Sciences

Levels of TLR4, MIF, IL-1β in umbilical cord blood of children with neonatal respiratory distress syndrome and their relationship with prognosis

QU Haixin, LIANG Junxia, YUAN Erwei, GAO Hongbo, KUI Xiaohua, GUO Weiping

2026, 46(1):  109-113.  doi:10.16352/j.issn.1001-6325.2026.01.0109

Asbtract ( 15 )   PDF (935KB) ( 2 )  

References | Related Articles | Metrics

Objective To detect the expression level of Toll-like receptor 4 (TLR4), macrophage migration inhibitory factor (MIF) and interleukin-1β (IL-1β) in umbilical cord blood, and to analyze their relationship with the prognosis of neonatal respiratory distress syndrome (NRDS). Methods From January 2023 to June 2024, 206 premature infants in our hospital were reviewed. Complying with the NRDS diagnostic criteria, they were assigned into NRDS group (n=120) and non-NRDS group (n=86). Complying with the prognosis of NRDS patients, they were assigned into a good prognosis group (n=90) and a poor prognosis group (n=30). ELISA method was applied to detect the expression levels of TLR4, IL-1β, and MIF in umbilical cord blood. Multivariate Logistic regression was applied to analyze the factors affecting the prognosis of NRDS. Receiver operating characteristic (ROC) curve was applied to analyze the efficacy of cord blood TLR4, MIF, and IL-1β in predicting poor prognosis in children with NRDS. Results Compared with the non-NRDS group, the level of TLR4, MIF and IL-1β in umbilical cord blood were significantly higher in the NRDS group (P＜0.05). Compared with the good prognosis group, the levels of TLR4, MIF and IL-1β in umbilical cord blood were greatly higher in the poor prognosis group (P＜0.05). Elevated levels of TLR4, MIF, and IL-1β in umbilical cord blood was a group of risk factors affecting for poor prognosis in children with NRDS (P＜0.05). The AUC of TLR4, MIF, IL-1β, and their combination in predicting the prognosis of NRDS children in umbilical cord blood was 0.843, 0.845, 0.850, and 0.976, respectively. The effectiveness of the combined prediction of the three indicators was greatly better than that of a single indicator (Z_{combination-TLR4}=3.841,Z_{combination-MIF}=3.615, Z_{combination-IL-1β}=2.452, P＜0.001, 0.001, 0.05). Conclusions The level of TLR4, MIF, and IL-1β in umbilical cord blood of children with NRDS is greatly elevated. The changes in their level of these factors are closely related to the prognosis of NRDS children. The combination of the three shows higher efficacy in predicting poor prognosis in NRDS children.

Clinical value of combined detection of serum sCD40L,ANXA1 and TIM-4 in neonatal bacterial sepsis

WANG Wei, JIA Yanmin, WANG Jing

2026, 46(1):  114-119.  doi:10.16352/j.issn.1001-6325.2026.01.0114

Asbtract ( 14 )   PDF (958KB) ( 4 )  

References | Related Articles | Metrics

Objective To investigate the expression and clinical significance of soluble cluster of differentiation 40 ligand (sCD40L),annexin A1 (ANXA1),T-cell immunoglobulin and mucin domain-containing protein 4 (TIM-4) in the serum of neonates with bacterial sepsis. Methods From September 2022 to March 2024,122 neonates with bacterial sepsis admitted to Shijiazhuang Fifth Hospital were included as the sepsis group, 122 newborns with common bacterial infection were selected as the infection group and 122 healthy newborns were selected as the control group. The level of sCD40L, ANXA1, and TIM-4 in serum was compared; multivariate logistic regression was used to analyze the influencing factors of bacterial sepsis; Receiver operating characteristic curve (ROC) curve was used to analyze the diagnostic value of serum sCD40L, ANXA1, and TIM-4 levels for bacterial sepsis. Results Compared with control group, the level of serum sCD40L,ANXA1 and TIM-4 was significantly higher in the sepsis group and infection group (P＜0.05); the level of serum sCD40L,ANXA1 and TIM-4 were obviously higher in the sepsis group (P＜0.05); The white blood cell count, CRP and SOFA scores of children in the sepsis group and infection group were all significantly higher (P＜0.05). Compared with the infection group, the white blood cell count, C-reactive protein(CRP)and Sequential Organ Failure Assessment(SOFA) scores of the sepsis group were obviously higher (P＜0.05). CRP,SOFA score,sCD40L,ANXA1,and TIM-4 were all influencing factors of bacterial sepsis (P＜0.05). The area under the curve (AUC) of serum sCD40L, ANXA1, and TIM-4 level for diagnosing bacterial sepsis was 0.744, 0.797, and 0.827, respectively. The AUC of the combined diagnosis of the three factors was 0.921,which was obviously higher than that of the single indicator diagnosis (Z_{three factors combination-sCD40L}=5.518,Z_{three factors combination-ANXA1}=4.389,Z_{three factors combination-TIM-4}=3.674,P＜0.001,0.001,0.001),the sensitivity of the combined diagnosis of the three was 87.70%,and the specificity was 79.51%. Conclusions The expression level of serum sCD40L, ANXA1, and TIM-4 in children with bacterial sepsis is obviously up-regulated and they are all influencing factors of bacterial sepsis. The combination of the three(triple detection) has high diagnostic value for bacterial sepsis.

Comparison of serum 25-hydroxyvitamin D levels in women at different stages of pregnancy in Lhasa

Nimadunzhu, Zhaxiyangzong, Bianzhen, Yixicuomu, Zhizhangzhuoma, ZHAN Mingjun, Basangyangji, QIN Xuzhen

2026, 46(1):  120-123.  doi:10.16352/j.issn.1001-6325.2026.01.0120

Asbtract ( 14 )   PDF (890KB) ( 5 )  

References | Related Articles | Metrics

Objective To investigate the serum 25-hydroxyvitamin D[25-(OH)D] concentration level of pregnant women in Lhasa, Tibet, and to observe the nutritional status of vitamin D(VD) in different pregnant women. Methods A total of 367 healthy pregnant women in obstetric outpatient clinic of Tibet Autonomous Region People's Hospital from July 2021 to April 2023 were selected as the study subjects, and 209 non-pregnant women of childbearing age who had undergone physical examination during the same period were selected as the normal control group, and the serum 25-(OH)D level was detected by Abbott chem.-iluminescence method, and the statistical analysis was carried out by SPSS statistical software. Results The overall serum level of 25-(OH)D in 367 pregnant women was 11.5(8.5, 17.3)ng/mL. VD adequacy, insufficiency, and deficiency accounted for 16.6%, 30.0%, and 53.4%, respectively. The overall serum level of 25-(OH)D in 209 healthy control women was 9.9(7.7, 13.6)ng/mL. VD adequacy, insufficiency, and deficiency accounted for 6.7%, 27.8%, and 65.5%, respectively. There was no significant difference in serum 25-(OH) D level between 9-13⁺⁶weeks and 15-20⁺⁶weeks pregnant women. Conclusions VD insufficiency or deficiency is not uncommon in pregnant women in Lhasa, and VD supplementation should be given during pregnancy preparation and pregnancy.

Mini Reviews

Advances in the mechanism of action of the ring finger proteins family in breast cancer

LI Binfeng, LYU Fayou, YI Huijun, LYU Lyu

2026, 46(1):  124-128.  doi:10.16352/j.issn.1001-6325.2026.01.0124

Asbtract ( 17 )   PDF (774KB) ( 3 )  

References | Related Articles | Metrics

The ring finger proteins(RNFs) family not only function as E3 ubiquitin ligases involved in protein degradation, but also plays crucial roles in regulating various cellular processes such as proliferation, apoptosis, DNA damage repair, tumor immune escape and epigenetic modification. RNFs promote breast cancer cell proliferation and survival by activating specific signaling pathways and modulating gene expression. By facilitating DNA damage repair, they contribute to genomic stability and enhance resistance to therapy. In the context of tumor immune evasion, RNFs mediate the ubiquitination of immune checkpoint molecules, thereby inhibiting anti-tumor immune responses. Furthermore, in epigenetic regulation, RNFs influence DNA methylation and histone modifications to suppress the expression of tumor suppressor genes, thus promoting cancer cell proliferation and drug resistance. Targeting RNFs may inhibit tumor growth and improve therapeutic outcomes in breast cancer.

Gut microbiota dysbiosis and diabetic cardiomyopathy

SUN Qingyi, CHEN Guo, FU Xiao, SUN Haijian, LU Qingbo

2026, 46(1):  129-133.  doi:10.16352/j.issn.1001-6325.2026.01.0129

Asbtract ( 18 )   PDF (770KB) ( 3 )  

References | Related Articles | Metrics

Dysregulation of gut microbiota and its metabolites contributes to diabetic cardiomyopathy (DCM) primarily through inflammation, oxidative stress, energy metabolism disturbances, insulin resistance, apoptosis and autophagy. Gut microbiota dysbiosis disrupts intestinal barrier function, triggering inflammation and oxidative stress, which exacerbate myocardial inflammation and damage. Additionally, gut microbiota modulates bile acid metabolism, influencing the activation of Takeda G protein-coupled receptor 5(TGR5), thereby affecting glucose and lipid metabolism as well as cardiac function. Metabolites such as trimethylamine N-oxide (TMAO) may activate inflammatory pathways and impair insulin signaling, reducing insulin sensitivity and exacerbating glucose metabolism disorders, further aggravating DCM. Targeting the gut microbiota composition and restoring the homeostasis of its metabolites may be potential strategy for the prevention and treatment of DCM.

Role of Sp1 posttranslational modification in atherosclerosis

ZHOU Qi, SUN Xutao, WU Siyu, QU Ying, SONG Yunjia

2026, 46(1):  134-138.  doi:10.16352/j.issn.1001-6325.2026.01.0134

Asbtract ( 23 )   PDF (770KB) ( 4 )  

References | Related Articles | Metrics

Specific proteins (Sp) are a well-known family of transcription factors. Sp1 as a key transcription factor, specific protein 1 (Sp1) regulates the pathological process of atherosclerosis (AS) through post-translational modification (PTMs). Phosphorylation and acetylating affect lipid metabolism, plaque stability, proliferation of vascular smooth muscle cells (VSMCs) and endothelial dysfunction by regulating DNA binding capacity and transcriptional activity. Phosphorylated Sp1 enhances the binding of ABCA1 and LDLR promoters to promote cholesterol efflux. Acetylated Sp1 up-regulates SR-BI expression and inhibits lipid accumulation by recruiting p300. Melatonin promotes collagen deposition through Akt/Sp1 pathway, and atorvastatin inhibits the binding of Sp1 and TLR2 to reduce the release of inflammatory factors. AngⅡ/PKC-ζ pathway drives PDGF-D expression to promote intimal thickening, while FGF-2/ERK pathway inhibits PDGFR-α transcription through Sp1 phosphorylation and delays plaque progression. S-sulfhydrylation and ubiquitination modifications improve vasodilatation and oxidative stress by stabilizing Sp1 and enhancing its binding to VEGFR-2. In this paper, the regulatory network of Sp1 PTMs in AS is analyzed, and potential therapeutic strategies targeting modification sites (such as Thr453/Ser702) and natural compounds (such as Cy3G and kaempferol) are proposed to provide theoretical basis for precise intervention of AS.

Research progress on neutrophil extracellular traps in sepsis-induced acute respiratory distress syndrome

LIU Tingting, GAO Ling, LI Xiaoyan

2026, 46(1):  139-143.  doi:10.16352/j.issn.1001-6325.2026.01.0139

Asbtract ( 22 )   PDF (770KB) ( 7 )  

References | Related Articles | Metrics

Neutrophil extracellular traps(NETs) are networks made of chromatin fibers released from polymorph nuclear neutrophils, mainly composed of DNA, histones and granule proteins. NETs are able to mediate downstream signaling pathways, which can activate inflammatory responses, leading to lung tissue injury. And can activate the coagulation pathway, promote microthrombus formation, lead to microcirculation disorders, and further aggravate the damage of lung tissue. These mechanisms of action revealed that NETs are risk factors for sepsis-induced acute respiratory distress syndrome(ARDS) progression, and that inhibition of the release of NETs has an important role in the mechanisms of delaying ARDS progression.

Medical Education

Effectiveness evaluation of combined CBL and TBL teaching in the radiology clerkship for students from eight-year program of clinical medicine

RUAN Shuaizhi, YAN Ruiyi, LIU Qixing, XU Xiang, JIA Mingnan, HUANG Xinying, WANG Qin, ZHANG Yan, WANG Fengdan, WANG Yining, FENG Feng

2026, 46(1):  144-149.  doi:10.16352/j.issn.1001-6325.2026.01.0144

Asbtract ( 19 )   PDF (762KB) ( 3 )  

References | Related Articles | Metrics

Objective To explore the teaching effects of case-based learning (CBL) and team-based learning (TBL) in students from eight-year program of clinical medicine at department of radiology. Methods A total of 89 students joined radiology clerkship training at Peking Union Medical College from July 2024 to February 2025. Eight radiology teaching sessions (24 class hours in total) were conducted according to organ system. Students were randomly divided into a control group (classic training program) and an experimental group (combined CBL and TBL methodology). After each session, questionnaires were used to collect data about students' learning outcomes and self-evaluation based satisfaction. Results Students in experimental were grouped significantly out-performed those in the control group in capacity building of clinical reasoning, logical analysis and learning feedback(P＜0.05). Additionally, presentation training in classroom was believed to be more effective than classic teaching in terms of listening in enhancing knowledge retention, motivation, and learning initiative (P＜0.05). Conclusions The combined application of CBL and TBL can significantly improve the clinical analysis capacity and learning satisfaction of eight-year medical students.

Impact of point-of-care ultrasound (POCUS) training on emergency physicians' capacities building in trauma assessment

LIU Yang, YANG Jing, ZHU Huadong, LIU Jihai, LI Yi, XU Jun, LIU Anlei

2026, 46(1):  150-154.  doi:10.16352/j.issn.1001-6325.2026.01.0150

Asbtract ( 16 )   PDF (921KB) ( 6 )  

References | Related Articles | Metrics

Objective To evaluate the effectiveness of point-of-care ultrasound (POCUS) training in improving emergency physicians' skill of trauma assessment, confidence, and clinical decision-making capability. Methods TThis was a prospective study involving 80 emergency physicians without POCUS training experience. Participants were randomly assigned to a structured POCUS training group and a conventional training group. Both groups underwent standardized pre- and post-intervention assessments, including theoretical knowledge tests, practical skill evaluations in simulated trauma scenarios, assessments of clinical decision-making accuracy, and self-rated confidence scoring. Results After the intervention, the POCUS group demonstrated significantly better performance than the control group in theoretical knowledge (88.3±6.4 vs. 80.0±5.5, P＜0.001), practical skills (84.9±7.0 vs. 75.3±6.9, P＜0.001), and self-confidence scores (median 8 vs. 6, P＜0.05). Although there was no statistically significant difference in post-training clinical decision-making accuracy between the two groups (median 8 vs. 7, P＞0.05), the POCUS group showed a significant improvement as compared to their baseline (median 8 vs. 6, P＜0.01). Conclusions Structured POCUS training significantly enhances emergency physicians' trauma assessment capacities and confidence. It demonstrates strong educational effectiveness and contributing the capacity of medical students in terms of faster and more accurate clinical decision-making.