Basic & Clinical Medicine ›› 2024, Vol. 44 ›› Issue (9): 1269-1273.doi: 10.16352/j.issn.1001-6325.2024.09.1269

• Original Articles • Previous Articles     Next Articles

Marein alleviates high glucose-induced damage of rat myocardial cell line H9c2

Zulipiya·ABULA1, ZHAO Qiang2, ZHANG Taimin3, LI Tian4,5*   

  1. 1. School of Traditional Chinese Medical; 3. Clinical Medicine Department; 4. Department of Histology and Embryology, School of Basic Medicine; 5. Xinjiang Key Laboratory of Molecular Biology of Endemic Diseases, Xinjiang Medical University, Urumqi 830001;
    2. Heart and Pan-vascular Medical Diagnosis and Treatment Center, People′s Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830001, China
  • Received:2024-01-03 Revised:2024-05-15 Online:2024-09-05 Published:2024-08-30
  • Contact: *292629092@qq.com

Abstract: Objective To study the effect of marein on the damage of rat myocardial cell line (H9c2) induced by high glucose. Methods H9c2 cells were divided into Ctrl group, high glucose (30 mmol/L) injury model group and marein (25, 50, 100 μmol/L) intervention group. The viability of H9c2 cells was detected by MTT method. Western blot was used to detect the protein expression level of LC-3Ⅱ/Ⅰ,p62 and mTOR. Results Compared with the Ctrl group, the viability of H9c2 cells in the model group was decreased (P< 0.01) and the viability of H9c2 cells in the marein group was significantly increased (P< 0.01). The protein expression of LC-3Ⅱ/Ⅰ in model group was decreased (P< 0.05) and the level of p62 and mTOR was increased (P< 0.01). The level of LC-3Ⅱ/Ⅰ in the marein group was increased, while the level of p62 and mTOR was decreased (P< 0.01). The protein expression was more obvious with the increase of concentration. Conclusions Marein can affect the expression of autophagy associated proteins in cardiomyocyte injury induced by high glucose.

Key words: marein, H9c2 cell, high-glucose injury, autophagy

CLC Number: