Balanophora polysaccharide protects against neuron damage in rat model of spinal cord injury

doi:10.16352/j.issn.1001-6325.2022.10.1509

Abstract

Abstract: Objective To investigate the effect of balanophora polysaccharide (BPS) on neuro-autophagy and its therapeutic effect on spinal cord injury. Methods The rats were randomly divided into sham operation group(sham, n=10),model group(SCI, n=15,which were established according to the Allen's weight-striking method), and the BPS treatment group(BPS, n=45),which were given intra-gastrically with low, medium and high dose of BPS solution for 14 days). The BBB scores were measured to evaluate motor function. Hematoxylin-Eosin and Nissl staining was used to observe the morphological changes. The expression of autophagy markers of Beclin 1, LC3-Ⅱ and p62 was detected by Western blot. Results Compared with the sham operation group,the curve of BBB score-time in SCI group was significantly lower and the injury was severer(P＜0.05). In SCI group, the dorsal white matter and central gray matter of spinal cord were seriously injured with obvious cavity. Further observation showed that the number of neurons in SCI group was significantly decreased; the structure of cell body was incomplete. The result of Western bolt showed that the relative change of LC3 Ⅱ /Ⅰ and p62 was up-regulated on day 1,7 and 14, the highest expression of both protein was found on day 7(P＜0.05). At the same time,Beclin 1 was decreased after injury with lowest expression on day 7(P＜0.05). The BBB scores of BPS group were increased with a dose-dependent manner as compared with SCI group. The cavity of dorsal white matter and central gray matter shrinked. The number of neurons was increased with intact morphology. The expression of Beclin 1 and LC3 Ⅱ/Ⅰ was increased significantly in BPS group, while p62 decreased as compared to SCI group(P＜0.05). Conclusions BPS has a well-demonstrated neuron-protective effect and the underlying mechanism might be related to the protection of injured nerve cells.

Key words: Balanophora polysaccharide, spinal cord injury, autophagy

CLC Number:

R285.5

ZHAO Fang-yu, QIN Xiao-li, HE Yi-duo, WANG Feng-jie, TANG Xian-e, PENG Xin-xin, CHEN Xian-bing. Balanophora polysaccharide protects against neuron damage in rat model of spinal cord injury[J]. Basic & Clinical Medicine, 2022, 42(10): 1509-1513.

References 15

[1]	Domingo A, Al-Yahya AA, Asiri Y, et al. A systematic review of the effects ofpharmacological agents on walking function in people with spinal cord injury[J]. J Neurotrauma, 2012, 29: 865-879.
[2]	Wang ZY, Lin JH, Muharram A,et al. Beclin-1-mediated autophagy protects spinal cord neurons against mechanical injury-induced apoptosis[J]. Apoptosis, 2014, 19: 933-945.
[3]	Lipinski MM, Wu J, Faden AI,et al. Function and mechanisms of autophagy in brain and spinal cord trauma[J]. Antioxid Redox Signal, 2015, 23: 565-577.
[4]	Zhou K, Sansur CA, Xu H,et al. The temporal pattern, flux, and function of autophagy in spinal cord injury[J]. Int J Mol Sci, 2017, 18:466-467.
[5]	赵秀,黄艳丽,朱春梅,等.药用植物筒鞘蛇菰的研究进展[J].南方农业,2019, 13:129-131.
[6]	张桃,谢雅,陈颖,等.筒鞘蛇菰提取物蛇菰多糖对D-半乳糖诱导大鼠肝损伤的保护作用及其机制[J].中国应用生理学, 2021, 37:276-280.
[7]	谢雅,陈颖,夏德尧,等.蛇菰多糖降低实验性肝损伤大鼠肝脏BDNF和TrkB的表达[J].基础医学与临床,2022,42:62-67.
[8]	Gruner JA. A monitored contusion model of spinal cord injury in the rat.[J]. J Neurotrauma, 1992,9: 123-126.
[9]	FournierAE, TakizawaBT, Strittmatter SM,et al. Rho kinase inhibition enhances axonal regeneration in the injured CNS[J]. J Neurosci, 2003, 23: 1416-1423.
[10]	Nikoletopoulou V, Papandreou ME, Tavernarakis N,et al. Autophagy in the physiology and pathology of the central nervous system[J]. Cell Death Differ, 2015, 22: 398-407.
[11]	Cherra SJ, Chu CT. Autophagy in neuroprotection and neurodegeneration: a question of balance[J]. Future Neurol, 2008, 3: 309-323.
[12]	刘丽耘,吴永灿,肖玮,等.中医药调控自噬流的研究进展[J].中药药理与临床, 2021, 37: 204-210.
[13]	Smith CM, Mayer JA, Duncan ID,et al. Autophagy promotes oligodendrocyte survival and function following dysmyelination in a long-lived myelin mutant[J]. J Neurosci, 2013, 33: 8088-8100.
[14]	Munoz-Galdeano T, Reigada D, Del Aguila A,et al. Cell specific changes of autophagy in a mouse model of contusive spinal cord injury[J]. Front Cell Neurosci, 2018, 12: 164-165.
[15]	Chen XB, Wang ZL, Yang QY, et al. Diosgenin glucoside protects against spinal cord injury by regulating autophagy and alleviating apoptosis[J]. Int J Mol Sci, 2018, 19:2274-2275.