Abstract: Objective To investigate whether the anti-TfR antibody would lead to increased ER stress, and if anti-TfR antibody may inhibite proliferation of glioma cell by targeting GRP78. Methods Western blot analysis was done to detecte the expression of GRP78 and CHOP after cells were treated with anti-TfR mAb. FCM was done to detecte the proliferation and apoptosis of cells after transfection with siRNA and treated with anti-TfR mAb. Results Compared with the control groups, the anti-TfR antibody elevated expression GRP78 and CHOP. After glioma cells transfected with small interfering RNA (siRNA) against GRP78 showed lower proliferation and higher apoptosis rate when incubated with anti-TfR antibody. Conclusion Anti-TfR antibody has triggered the ER stress response, and that this effect is achieved via the siRNA against GRP78 for glioma cell proliferation and survival.

Key words: TfR, Glioma, GRP78