Abstract: Objective Learning the influence of PAPD4 gene deletion on mouse behaviors and physiologies. Methods Mice were divided into four groups by sex and genotype: male control, male knockout, female control and female knockout. Open field test, Morris water maze, blood glucose test, blood pressure test, T maze, sucrose preference test and forced swimming test. Results In open field test, the locomotion velocity (p<0.05) and the total distance (p<0.001) of female KO mouse were significantly slower than female controls. In Morris water maze test, the locomotion velocity of KO mouse was lower than normal controls. Specifically, the velocity of female KO mouse was significantly slower than female controls in the sixth and ninth trial (p6<0.05, p9<0.05), and the velocity of male KO mouse was also significantly slower than male controls in the fourth and ninth trial (p4<0.01, p9<0.05). Otherwise, the blood glucose of male KO mouse was significantly lower than male normal controls at ad libitum state(p<0.05); but after 16 hours food deprivation, the blood glucose of male KO mouse was significantly higher than male controls(p<0.05), and analyzing the blood glucose change of before-after feeding, we found the change of male KO mouse was significantly lower than male controls(p<0.01). Conclusion Deleting of PAPD4 gene affects locomotion ability of mouse, and this gene may participate in regulation of blood glucose level in male mouse.

Key words: PAPD4, movement ability, polyadenylation, blood glucose,