Abstract: Objective To investigate the effect of moxifloxacin(MXF） on inflammatory reactionof mice abdominal peritoneal macrophage induced by LPS. Methods The levels of TLR4、SPHK1 and NF-κB mRNA were determined by realtime-PCR;The relative levels of TLR4、SPHK1 and NF-κB were determined by Western blot. The levels of TNF-αand IL-1 secreted by macrophages were detected by ELISA. Results At low and medium concentration（8，16mg/L）of the MXF have inhibitory effect on the increasing of TLR4, SPHK1, NF-κB expression level and cell supernatant of TNF-α and IL-1 in LPS stimulation of mice peritoneal macrophages. And high concentration (64 mg/L) of the MXF up to promote the role of inflammation. Conclusions The MXF of low concentration and medium concentration can inhibit the inflammatory response in LPS stimulation of mice peritoneal macrophages, and high concentration of the MXF showed contrary effects.

Key words: Moxifloxacin, Macrophages, lipopolysaccharide, Toll-like receptor 4, Sphingosine kinase 1