Advances in CRISPR/Cas9 gene-editing system

Basic & Clinical Medicine ›› 2018, Vol. 38 ›› Issue (4): 543-547.

Advances in CRISPR/Cas9 gene-editing system

Cheng CHENG¹,Peng-cheng SHU¹,Xiao-zhong PENG

Received:2017-11-30 Revised:2018-01-17 Online:2018-04-05 Published:2018-03-27
Contact: Xiao-zhong PENG E-mail:peng_xiaozhong@163.com
Supported by:
The National Key Research and Development Program of China;The National Key Basic Research Program;National Natural Science Foundation of China;CAMS Innovation Fund for Medical Sciences

Abstract

Abstract: CRISPR/Cas9 gene-editing system has been broadly used in various fields of bioscience and medicine in recent years. It can be guided by RNA to specific DNA site thus achieving targeted gene editing. Off-target effect and editing efficiency remain two crucial issues facing the system. Currently, a number of researches have been focused on the optimization of the system by reducing off-target effects and increasing editing efficiency, which will help enhance its safety and expand its application.

Key words: CRISPR/Cas9, gene editing, off-target effect, editing efficiency

CLC Number:

Cheng CHENG Peng-cheng SHU Xiao-zhong PENG. Advances in CRISPR/Cas9 gene-editing system[J]. Basic & Clinical Medicine, 2018, 38(4): 543-547.

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Advances in CRISPR/Cas9 gene-editing system

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