Abstract: Objective: To explore the role of resveratrol (RES) on cardiomyocyte hypertrophy of rat induced by isoproternol (ISO) and the effect of Res on the expression of GRP78 and GRP94 in endoplasmic reticulum stress of cardiomyocyte hypertrophy. Methods: Hypertrophic model of cardiomyocytes was induced by ISO. Cardiomyocytes can be divided into four group: control group, the model group, RES+ISO group and RES group. Hypertrophy status of cardiomyocytes was determined by Leica 2Q500 image analysis system measuring the cell surface area and the gene expression of ANP. The content of LDH and MDA was measured in different groups, and the protein expressions of GRP78 and GRP94 were detected by Western blot. Results：Compared with control group, ISO induced cardiomyocytes hypertrophy, endoplasmic reticulum stress related factors GRP78 and GRP94 protein expression were increased, compared with ISO group, RES intervention could effectively suppress the cardiomyocytes hypertrophy induced by ISO, reduce the protein expression of GRP78 and GRP94, at the same time, reduce lactate dehydrogenase (LDH) and malondialdehyde (MDA) release in cell medium. Conclusion：The results suggest that treatment of RES may protect cardiomyocytes hypertrophy, which is partially mediated by inhibiting the expression of ERS factors GRP78 and GRP94 . .

Key words: cardiomyocyte hypertrophy, endoplasmic reticulum stress, resveratrol, GRP78, GRP94