Abstract: Objective To investigate the diagnostic value of single and combined test of seven serum autoantibodies to tumor related antigens (NY-ESO-1，p53，CDK2, AnnexinA2, IMP1, Ubiquilin1 and PGAM1) in non-small-cell lung carcinoma. Methods Serum levels of seven autoantibodies in NSCLC patients (48) and health controls (45) were measured simultaneously by multiplexed Luminex assays. Receiving operating characteristic (ROC) curves were used to analyze the diagnostic accuracy in single and combined tests. Results 1) The level of seven autoantibodies in serum were higher in patients with NSCLC than that in health controls (p<0.05)； 2) The analysis of ROC curves showed that the AUC of NY-ESO-1 and p53 were more than 0.7, while the sensitivities of AnnexinA2 was 88.89 %； 3) The AUC, sensitivity and specificity of combined assay of seven autoantibodies for NSCLC were 0.812, 68.75 % and 91.11 %. Conclusions All these seven autoantibodies have certain significance in the diagnosis of NSCLC and combination of seven autoantibodies can enhance the diagnostic accuracy for NSCLC.

Key words: NSCLC, autoantibodies, Luminex, combined test, diagnose