Abstract: Objective To explore the mechanisms of aging，hippocampal tissue of senescence accelerated mouse(SAM)were used to screen aging related miRNAs. Methods SAMP8 and SAMR1 (4-, 8-, 12- month old) were randomly selected 3with each group, extracting RNA from hippocampal tissue for miRNA microarray. To verify the relative quantitative expression of miRNAs, SYBR Green was used as a dye for the Real-time PCR and snRNA U6 was used as control. Results Based on the validation of real-time PCR, here we present the microarray results which have better repeatability and authenticity. Comprehensive analysis of three repeat miRNA array data showed that 7 miRNAs, 8 miRNAsand 3 miRNAs are dynamically expressed in the 4- month/8- months, 8- month/12- months and 4- month/12- month hippocampal tissues, respectively. Only miR-9* showed an obviously differential expression pattern in both the three stages. Conclusions The differentially expressed miRNAs in SAMP8 and SAMR1 mice could play an important role in the aging process.

Key words: ageing, miRNAs, senescence accelerated mouse，hippocampus