Abstract: Object To study the effect of glucose on the mouse skin fibroblast proliferation. Methods The cells cultured in different concentrations of glucose medium including 5.5mmol/L、15mmol/L、25mmol/L, and tested the cells proliferation by MTT. The formation of translation initiation factor 4F complex was analyzed by 7-MGTP pulldown test and the mTORC1 signal pathway was analyzed by Western blot. Result In contrasted with 5.5mmol/L, when the concentration of glucose medium was 15mmol/L, it promoted the cell proliferation and the translation initiation, activated the mTORC1 signal pathway. In contrasted with 5.5mmol/L, 25mmol/L inhibited the cell proliferation and the translation initiation, inactivated the mTORC1 signal pathway (included the 4EBP1 related with cell proliferation and the Akt related with cell survival), but the S6K1 was over activatied. Conclusion Glucose controls the fibroblast proliferation through the mTORC1 signal pathway.

Key words: Key words: chronic wound healing, glucose, fibroblast, eIF4F, mTORC1