Abstract: Objective The study aimed to make clear the relationship between MyD88 and the resistance of ovarian cancer cell to paclitaxel. Methods After knocking-down and over-expressing MyD88 in A2780/Taxol cell line, qRT-PCR and western blot were performed to test the expression change of MyD88 on mRNA and protein level compared with control group, respectively. CCK-8 assay was executed to detect the change of resistance to paclitaxel in A2780/Taxol cells. Flow cytometry was performed to test the affection to Cell cycle and apoptosis of A2780/Taxol cell line. Results By means of inhibiting MyD88, we observed that resistance of A2780/Taxol to paclitaxel declined obviously, and anti-apoptotic significantly weakened. Inversely, over-expression of MyD88 increased cells resistance to paclitaxel. Conclusion Down-regulation of MyD88 increased sensitivity of ovarian cancer cell to paclitaxel, which provide new ideas and targets of gene therapy for the clinical treatment of ovarian cancer resistant patients.

Key words: Key words: ovarian cancer, taxol-resistant, MyD88