Abstract: Background: Even though there are so many researches on grape seed procyanidin, its protective effect on chemical hepatic injury in mouse has seldom been explored. Objective：To investigate the protective effect of grape seed procyanidin on chemical hepatic injury in mouse Methods：Copy acute chemical hepatic injury in the mouse with alcohol and carbon tetrachloride respectively as the model group; treat the mouse with different concentrations of grape seed procyanidin (GSP) by intragastric administration as the treatment group; then detect the serum concentration of alanine aminotransferase (ALT) and aspartate aminotransferase (AST); analyze the content of superoxide dismutase (SOD) and malondialdehyde (MDA) and observe the pathological change in the hepatic tissue. Results：Two types of acute chemical hepatic injury model were obtained successfully and all index in the model groups differed from the control group significantly (P＜0.01). Compared with the model groups, the content of MDA and serum concentration of ALT and AST in the treatment group decreased dose-dependently (P＜0.05), but SOD increased in a dose-dependent way. Conclusions：GSP has some protective effect on the alcohol or carbon tetrachloride-induced chemical hepatic injury in the mouse. To some extent, GSP protects the liver in a dose-dependent way.

Key words: Grape seed procyanidin , ALT, AST, SOD, MDA