Circulating microparticles from acute coronary syndrome patients complicated with type 2 diabetes induce endothelial cell injury in rats

doi:10.16352/j.issn.1001-6325.2026.03.0381

Abstract

Abstract: Objective To investigate the effects of circulating microparticulates (MPs) on vascular endothelium injury in patients with acute coronary syndrome (ACS) and type 2 diabetes (T2DM). Methods MPs were extracted from control (n=15), ACS patients (ACS, n=18), T2DM patients (T2DM, n=18) and ACS patients with T2DM (ACS+T2DM group, n=18). After incubation of rat thoracic artery tissue with MPs, the effects of MPs on endothelial dependent vasodilatation was detected with biological assay, expression of caveolin-1, coupling of endothelial nitric oxide synthase (eNOS) with heat shock protein 90 (Hsp90) was detected with Western blot. The changes in nitric oxide (NO) and superoxide anion were examined with chemical assay. Results The MPs concentration in ACS+T2DM patients samples was higher than that in control (P＜0.05). However, compared with ACS and T2DM patients, there was no statistically significant difference in MPs concentration. Compared with the control, regardless of ACS, T2DM and ACS+T2DM, their MPs inhibited endothelial dependent vasodilatation function, eNOS and Hsp90 coupling and NO production and stimulated expression of caveolin-1 and generation(P＜0.05). However, compared with ACS and T2DM patients, the effects of MPs were stronger in ACS+T2DM(P＜0.05). Conclusions The changes in the quality (or function) of MPs in ACS+T2DM lead to damage of rat vascular endothelium by MPs. MPs are potential marker molecules to support diagnosis of endothelial dysfunction.

Key words: acute coronary syndrome, circulation microparticles, endothelial dysfunction, vascular injury, oxidative stress

CLC Number:

CHANG Fengjun, WANG Xing, YANG Yujuan, LI Hongwen. Circulating microparticles from acute coronary syndrome patients complicated with type 2 diabetes induce endothelial cell injury in rats[J]. Basic & Clinical Medicine, 2026, 46(3): 381-387.

References

[1] 高晓倩,于华惠,刘圣,等.血尿素氮水平对急性冠状动脉综合征患者PCI术后MACE的预测价值[J].基础医学与临床,2024,44:606-612.
[2] Kong P, Cui ZY, Huang XF, et al. Inflammation and atherosclerosis: signaling pathways and therapeuticintervention[J]. Signal Transduct Target Ther, 2022,22,7:131. doi: 10.1038/s41392-022-00955-7.
[3] Willems R, Pil L, Lambrinou CP, et al. Feel4 diabetes research group. methodology of the health economic evaluation of the Feel4 diabetes-study[J]. BMC Endocr Disord, 2020,12,20:14. doi: 10.1186/s12902-019-0471-3.
[4] Shin MA, Oh S, Kim MC, et al. Time to presentation and mortality outcomes among patients with diabetes and acute myocardial infarction[J]. Korean J Intern Med, 2024,39:110-122.
[5] Abdolalian M, Khalaf-Adeli E, Yari F, et al. Correlations between the circulating level of cell-derived microparticles and surgical variables in heart valve surgery with cardiopulmonary bypass[J]. J Tehran Heart Cent, 2022,17:134-139.
[6] Han WQ, Chang FJ, Wang QR, et al. Microparticles from patients with the acute coronary syndrome impair vasodilatation by inhibiting the Akt/eNOS-Hsp90 signaling pathway[J]. Cardiology, 2015,132: 252-60.
[7] Cheng G, Shan XF, Wang XL, et al. Endothelial damage effects of circulating microparticles from patients with stable angina are reduced by aspirin through ERK/p38 MAPKs pathways[J]. Cardiovasc Ther, 2017, 35. doi: 10.1111/1755-5922.12273.
[8] Li Z, Zhang W, Wang QR, et al. Effect of thrombolysis on circulating microparticles in patients with ST-segment elevation myocardial infarction[J]. Cardiovasc Ther, 2023,2023:5559368. doi: 10.1155/2023/5559368.
[9] Biasucci LM, Porto I, Di Vito L, et al. Differences in microparticle release in patients with acute coronary syndrome and stable angina[J]. Circ J, 2012,76:2174-2182.
[10] Bernal-Mizrachi L, Jy W, Fierro C, et al. Endothelial microparticles correlate with high-risk angiographic lesions in acute coronary syndromes[J]. Int J Cardiol, 2004,97:439-446.
[11] 余姿漫,赵炳佳,杨丹.JAK-STAT信号通路与糖尿病并发症的研究进展[J].基础医学与临床,2023,43:1590-1593.
[12] Mannar V, Boro H, Patel D, et al. Epigenetics of the pathogenesis and complications of type 2 diabetes mellitus[J]. touchREV Endocrinol, 2023,19:46-53.
[13] Mineo C, Shaul PW. Regulation of eNOS in caveolae[J]. Adv Exp Med Biol, 2012,729:51-62.
[14] Zhao YY, Liu Y, Stan RV, et al. Defects in caveolin-1 cause dilated cardiomyopathy and pulmonary hypertension in knockout mice[J]. Proc Natl Acad Sci U S A, 2002,99:11375- 11380.
[15] Petrova NV, Tarasov SA, Epstein OI, et al. Highly diluted antibodies to eNOS restore endothelium function in aortic rings from hypertensive rats[J]. Dose Response, 2022,20:1559325822 1099281. doi: 10.1177/15593258221099281.