Abstract: Objective To study the correlation of serum miR-185-3p and miR-3194-5p with lesion severity of coronary artery. Methods A total of 88 patients with coronary artery disease, diagnosed and treated at the First Affiliated Hospital of Henan University of Science and Technology between January 2021 and July 2024, were enrolled as the research group. This group comprised 40 patients in the low-score subgroup and 48 in the high-score subgroup. The control group consisted of 36 healthy individuals undergoing physical examinations during the same period. The level of miR-185-3p and miR-3194-5p in serum was detected using real-time fluorescence quantitative PCR (RT- qPCR) method. Pearson correlation analysis was applied to assess the correlation between serum miR-185-3p and miR-3194-5p levels in the research group. The correlation between the levels of miR-185-3p and miR-3194-5p with the severity of coronary artery disease were analyzed using Spearman correlation analysis. Logistic regression was applied to analyze the influencing factors of high-score coronary heart disease lesions. ROC curve was applied to analyze the diagnostic value of serum miR-185-3p and miR-3194-5p levels for high-score lesions in clinical coronary heart disease. Results The serum miR-185-3p level in the control group was significantly lower than that in the research group, and the miR-185-3p level in the low-score group was lower than that in the high-score group (P＜0.05). The miR-3194-5p level in the research group was significantly lower than that in the control group, and the miR-3194-5p level in the high-score group was lower than that in the low-score group (P＜0.05). Serum miR-185-3p and miR-3194-5p in the research group were negatively correlated (P＜0.05), and both were correlated with the severity of coronary artery disease (P＜0.05). Logistic regression analysis showed that the history of diabetes, hypertension, apolipoprotein B, fasting blood glucose, high-sensitivity C-reactive protein, serum miR-185-3p and miR-3194-5p were all the influencing factors for the occurrence of coronary heart disease with high score (P＜0.05). The area under the curve (AUC) for the combined diagnosis of miR-185-3p and miR-3194-5p in high-score coronary heart disease lesions was significantly higher than that for their individual diagnosis (P＜0.05). Conclusions The serum miR-185-3p level in the research group is significantly higher than that in the control group, and the miR-3194-5p level is lower than the control group. The serum miR-185-3p level is positively correlated with the severity of coronary artery disease, while the serum miR-3194-5p level is negatively correlated with the severity of coronary artery disease. The combined detection of the two is of great significance for the clinical diagnosis of high-score lesions in coronary heart disease.

Key words: coronary heart disease, miR-185-3p, miR-3194-5p, severity of coronary artery disease