Abstract: Objective Exploring the effectiveness of humanities and professional ethics with experimental technology teaching in the course of “Molecular Biology Experiments of the Gene”. Methods Totally 101 students attending the course in the academic year 2024-2025 from Peking Union Medical College were selected as the research subjects. Based on students′ test scores, classroom performance, quality of experimental reports, and feedback from surveys, the effectiveness of construction with humanities and professional ethics in the course were evaluated. Results The students′ test scores have improved, especially the proportion of outstanding students has increased. The accuracy rate of students answering the humanities and professional ethics test questions has reached 98.86%. The success rate of experiments and the quality of experimental reports have significantly improved compared to the previous students. Students′ evaluation of the course has significantly improved. Conclusions Integrating the humanities and professional ethics in the course construction has achieved significant results, including test score, classroom performance, and post class evaluation.

Key words: Molecular Biology Experiments of the gene, postgraduate teaching, cultivation of humanities and professional ethics