Exosomes derived from bone marrow mesenchymal stem cells alleviate hypoxia/reoxygenation-induced cardiomyocyte injury

doi:10.16352/j.issn.1001-6325.2025.12.1557

Abstract

Abstract: Objective To explore the effects and mechanisms of bone marrow mesenchymal stem cell(BMMSC)-derived exosomes (BMMSC-Exo) on hypoxia/reoxygenation (H/R)-induced injury in rat cardiomyocyte cell line (H9c2). Methods BMMSC-Exosomes were isolated by ultracentrifugation. The cells were divided into three groups: control,H/R,and H/R+BMMSC-Exo (H/R+Exo). A hypoxia/reoxygenation (H/R) injury model was established by exposing cells to 12 hours of hypoxia followed by 6 hours of reoxygenation. Flow cytometry was used to detect cell apoptosis,DHE staining was used to assess cellular ROS levels,JC-1 immunofluorescence staining was used to evaluate mitochondrial membrane potential,and Western blot was used to detect mitochondrial autophagy-related proteins. Results BMMSC-Exo treatment significantly alleviated oxidative stress,restored mitochondrial membrane potential,reduced mitochondrial autophagy levels,and effectively decreased cardiomyocyte apoptosis. Conclusions Bone marrow mesenchymal stem cell-derived exosomes alleviate H/R-induced cardiomyocyte injury.

Key words: exosomes, hypoxia/reoxygenation, oxidative stress, mitochondria, apoptosis

CLC Number:

R541.4

WEN Wen, LIU Chenxi, CHEN Shuangjing, LU Xiaojiong, JIN Zhitao, ZHANG Zheng. Exosomes derived from bone marrow mesenchymal stem cells alleviate hypoxia/reoxygenation-induced cardiomyocyte injury[J]. Basic & Clinical Medicine, 2025, 45(12): 1557-1564.

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