Silencing Fcgr3 decreases SiO2-induced inflammatory cytokines expression in mouse alveolar macrophage cell line MH-S

doi:10.16352/j.issn.1001-6325.2023.06.0904

Abstract

Abstract: Objective To investigate the effect of receptor Ⅲ for the Fc region of immunoglobulin G (FcγRⅢ) on SiO₂-induced inflammatory factors expression in the mouse alveolar macrophage cell line MH-S. Methods The lentivirus plasmids with Fcgr3 shRNA were constructed; human embryonic kidney cell line 293T was incubated for packaging of the lentivirus for silencing FcγRⅢ in MH-S. Quantitative polymerase chain reaction (qPCR) and Western blot were used to detect the transcription level and protein expression of FcγRⅢ in MH-S. MH-S cells were divided into MH-S+sh-NC, MH-S+SiO₂+sh-NC, MH-S+sh-Fcgr3, MH-S+SiO₂+sh-Fcgr3, and the SiO₂ stimulation condition was incubation in a concentration of 100 mg/L for 12 h. Transcription levels of inflammatory cytokines was detected by qPCR, and the content of inflammatory cytokines in cell culture supernatants were detected by enzyme-linked immunosorbent assay (ELISA), including tumor necrosis factor (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6). Results The stable Fcgr3-silencing cell line was established after lentivirus infection. Transcription level and protein expression of FcγRⅢ in stable Fcgr3-silencing cell line were lower than that in control group (P＜0.05). The transcription level of Tnf,Il1b and Il6 was significantly increased after SiO₂ stimulation as compared with the control group (P＜0.05), and significantly decreased after Fcgr3 silencing (P＜0.05). The secretion of TNF-α and IL-6 was obviously increased after SiO₂ stimulation (P＜0.05), and significantly decreased after Fcgr3 silencing (P＜0.05). Conclusions Silencing Fcgr3 may decrease the expression of inflammatory cytokines induced by SiO₂ in alveolar macrophages.

Key words: receptor Ⅲ for the Fc region of immunoglobulin G, macrophage, inflammatory cytokines

CLC Number:

R392

LI Xiaona, QI Xianmei, ZHANG Tiantian, WANG Jing. Silencing Fcgr3 decreases SiO₂-induced inflammatory cytokines expression in mouse alveolar macrophage cell line MH-S[J]. Basic & Clinical Medicine, 2023, 43(6): 904-908.

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Silencing Fcgr3 decreases SiO₂-induced inflammatory cytokines expression in mouse alveolar macrophage cell line MH-S

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