Lnc-SLC2A12-10:1 silencing inhibits the proliferation, migration and invasion of gastric cancer cell line AGS

doi:10.16352/j.issn.1001-6325.2022.07.1071

Abstract

Abstract: Objective To investigate the effect of long non-coding RNA(lncRNA) lnc-SLC2A12-10:1 silencing on the proliferation, migration and invasion of gastric cancer cell line AGS. Methods Gene interference technology was used to construct the lnc-SLC2A12-10:1 silenced gastric cancer cell strain AGS, and RT-qPCR was used to verify the interference efficiency by lnc-SLC2A12-10:1 silencing. The proliferation of AGS cell was examined by CCK-8 method. The cell migration and invasion were evaluated by wound healing and Transwell assay. Bioinformatics prediction software was applied to predict lnc-SLC2A12-10:1 targeting miRNA and to verify them with RT-qPCR. Results Lnc-SLC2A12-10:1 shRNA effectively silenced expression of lnc-SLC2A12-10:1, and lnc-SLC2A12-10:1 silencing inhibited the proliferation of gastric cancer cells (P＜0.05). Lnc-SLC2A12-10:1 silencing also inhibited the migration and invasion of AGS cells (P＜0.05). It had been demonstrated that lnc-SLC2A12-10:1 targeting miRNAs might be hsa-miR-105-5p and hsa-miR-150-3p. Conclusions Lnc-SLC2A12-10:1 silencing can significantly inhibit the proliferation, migration and invasion of AGS cells.

Key words: long noncoding RNA, gastric cancer, proliferation, migration

CLC Number:

LIU Lei, LI Shi-bao, ZHANG Hao-liang. Lnc-SLC2A12-10:1 silencing inhibits the proliferation, migration and invasion of gastric cancer cell line AGS[J]. Basic & Clinical Medicine, 2022, 42(7): 1071-1076.

References 14

[1]	Chen W, Zheng R, Baade PD, et al. Cancer statistics in China, 2015[J]. CA Cancer J Clin, 2016, 66:115-132.
[2]	Sumiyama K. Past and current trends in endoscopic diagnosis for early stage gastric cancer in Japan[J]. Gastric Cancer, 2017, 20:20-27.
[3]	Pant S, Hilton H, Burczynski ME. The multifaceted exosome: biogenesis, role in normal and aberrant cellular function, and frontiers for pharmacological and biomarker opportunities[J]. Biochem Pharmacol, 2012, 83:1484-1494.
[4]	Li Q, Shao Y, Zhang X, et al. Plasma long noncoding RNA protected by exosomes as a potential stable bio-marker for gastric cancer[J]. Tumour Biol, 2015, 36:2007-2012.
[5]	Elsayed ET, Salem PE, Darwish AM, et al. Plasma long non-coding RNA HOTAIR as a potential biomarker for gastric cancer[J]. Int J Biol Markers, 2018:1724600818760244. doi: 10.1177/1724600818760244.
[6]	Yan J, Zhang Y, She Q, et al. Long noncoding RNA H19/miR-675 axis promotes gastric cancer via FADD/caspase 8/caspase 3 signaling pathway[J]. Cell Physiol Biochem, 2017, 42:2364-2376.
[7]	Peng W, Si S, Zhang Q, et al. Long non-coding RNA MEG3 functions as a competing endogenous RNA to regulate gastric cancer progression[J]. J Exp Clin Cancer Res, 2015, 34:79.doi:10.1186/s13046-015-0197-7.
[8]	Chang L, Li C, Lan T, et al. Decreased expression of long non-coding RNA GAS5 indicates a poor prognosis and promotes cell proliferation and invasion in hepatocellular carcinoma by regulating vimentin[J]. Mol Med Rep, 2016, 13:1541-1550.
[9]	Zheng P, Zhang H, Gao H, et al. Plasma exosomal long noncoding RNA lnc-SLC2A12-10:1 as a novel diagnostic biomarker for gastric cancer[J]. Onco Targets Ther, 2020, 13:4009-4018.
[10]	Pan T, Yu Z, Jin Z, et al. Tumor suppressor lnc-CTSLP4 inhibits EMT and metastasis of gastric cancer by attenuating HNRNPAB-dependent Snail transcription[J]. Mol Ther Nucleic Acids, 2021, 23:1288-1303.
[11]	Xiao J, Lin L, Luo D, et al. Long noncoding RNA TRPM2-AS acts as a microRNA sponge of miR-612 to promote gastric cancer progression and radioresistance[J]. Oncogenesis, 2020, 9:29.doi:10.1038/s41389-020-0215-2.
[12]	Naderi-Meshkin H, Lai X, Amirkhah R, et al. Exosomal lncRNAs and cancer: connecting the missing links[J]. Bioinformatics, 2019, 35:352-360.
[13]	田小星, 秦溧嫔, 李茜. 长链非编码RNA TINCR 通过miR-7调控肝癌细胞系Huh7侵袭和迁移[J]. 基础医学与临床, 2020, 40: 1041-1046.
[14]	Kim MS, Pinto SM, Getnet D, et al. A draft map of the human proteome[J]. Nature, 2014, 509:575-581.

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