Down-regulation of lncRNA RHPN1-AS1 inhibits proliferation and migration of human bladder cancer cell line T24

doi:10.16352/j.issn.1001-6325.2022.06.004

Abstract

Abstract: Objective To explore the effect of interfering with lncRNA RHPN1-AS1 on the biological behavior of bladder cancer cell T24 and its mechanism. Methods RT-qPCR method was used to detect the expression of RHPN1-AS1 and miR-149-3p by bladder cancer tissues and adjacent tissues.si-NC,si-RHPN1-AS1, si-RHPN1-AS1,anti-miR-NC, si-RHPN1-AS1 and miR-149-3p inhibitor were transfected into bladder cancer cell T24. The expression of RHPN1-AS1 and miR-149-3p in T24 cells were examined by RT-qPCR.MTT, plate colony formation experiment, scratch experiment, flow cytometry were used to detect cell proliferation, migration ability and cell apoptosis rate respectively. The dual luciferase reporter experiment was used to detect the targeting relationship between RHPN1-AS1 and miR-149-3p. Bax and Bcl-2 protein expression was assessed using Western blot. Results Compared with adjacent tissues, the expression of RHPN1-AS1 in bladder cancer tissue was increased(P＜0.05) and the expression of miR-149-3p was decreased (P＜0.05). Compared with the si-NC group, transfection of si-RHPN1-AS1 significantly reduced cell proliferation, scratch healing rate and the protein level of Bcl-2(P＜0.05), reduced the number of clones (P＜0.05)and increased apoptosis rate and the protein level of Bax(P＜0.05). Compared with the si-RHPN1-AS1+anti-miR-NC group, transfection of si-RHPN1-AS1 and miR-149-3p inhibitor significantly increased cell proliferation, scratch healing rate and the protein level of Bcl-2 (P＜0.05) increased the number of clones formed (P＜0.05), reduced the apoptosis rate and the protein level of Bax (P＜0.05). Conclusions Interfering with the expression of RHPN1-AS1 may inhibit the proliferation and migration of bladder cancer cells and may promote cell apoptosis by up-regulating miR-149-3p.

Key words: lncRNA RHPN1-AS1, miR-149-3p, bladder cancer, proliferation, migration

CLC Number:

R737.1

BAI Bing, ZHANG Hai-fang, YANG Qing-liang, QIN Yue, ZHOU Chen-long, SONG Ge, WANG Ying. Down-regulation of lncRNA RHPN1-AS1 inhibits proliferation and migration of human bladder cancer cell line T24[J]. Basic & Clinical Medicine, 2022, 42(6): 940-944.

References

[1] 黄一珂, 刁思军, 梁平, 等. 靶向沉默膀胱癌T24细胞系VEGF对树突状细胞分化成熟及免疫功能的影响[J]. 基础医学与临床, 2018, 38: 57-62.
[2] Luo H, Xu C, Le W, et al. lncRNA CASC11 promotes cancer cell proliferation in bladder cancer through miRNA-150[J]. J Cell Biochem, 2019, 120: 13487-13493.
[3] Zhuang C, Ma Q, Zhuang C, et al. LncRNA GClnc1 promotes proliferation and invasion of bladder cancer through activation of MYC[J]. FASEB J, 2019, 33: 11045-11059.
[4] Li HJ, Sun XM, Li ZK, et al. LncRNA UCA1 promotes mitochondrial function of bladder cancer via the miR-195/ARL2 signaling pathway[J]. Cell Physiol Biochem, 2017, 43: 2548-2561.
[5] Duan H, Li X, Chen Y, et al. LncRNA RHPN1-AS1 promoted cell proliferation, invasion and migration in cervical cancer via the modulation of miR-299-3p/FGF2 axis[J]. Life Sci, 2019, 239: 116856-116866.
[6] Qi H, Xiao Z, Wang Y. Long non-coding RNA LINC00665 gastric cancer tumorigenesis by regulation miR-149-3p/RNF2 axis[J]. Onco Targets Ther, 2019, 12: 6981-6990.
[7] Zhao L, Liu T, Zhang X, et al. lncRNA RHPN1-AS1 promotes ovarian cancer growth and invasiveness through inhibiting miR-1299[J]. Onco Targets Ther, 2020, 13: 5337-5344.
[8] Zheng W, Li H, Zhang H, et al. Long noncoding RNA RHPN1-AS1 promotes colorectal cancer progression via targeting miR-7-5p/OGT axis[J]. Cancer Cell Int, 2020, 20: 54-64.
[9] Liu G, Zhao X, Zhou J, et al. Long non-coding RNA MEG3 suppresses the development of bladder urothelial carcinoma by regulating miR-96 and TPM1[J]. Cancer Biol Ther, 2018, 19: 1039-1056.
[10] Chen D, Zhang M, Ruan J, et al. The long non-coding RNA HOXA11-AS promotes epithelial mesenchymal transition by sponging miR-149-3p in Colorectal Cancer[J]. J Cancer, 2020, 11: 6050-6058.
[11] Liang Y, Hou L, Li L, et al. Dichloroacetate restores colorectal cancer chemosensitivity through the p53/miR-149-3p/PDK2-mediated glucose metabolic pathway[J]. Oncogene, 2020, 39: 469-485.
[12] Liu Y, Yang C, Zhao Y, et al. Overexpressed methyltransferase-like 1 (METTL1) increased chemosensitivity of colon cancer cells to cisplatin by regulating miR-149-3p/S100A4/p53 axis[J]. Aging (Albany NY), 2019, 11: 12328-12344.

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