Toll-like receptor 3 activation combined with INFα inhibits the expression of DISC1 in hippocampal neurons of mice

doi:10.16352/j.issn.1001-6325.2022.12.1855

Basic & Clinical Medicine ›› 2022, Vol. 42 ›› Issue (12): 1855-1860.doi: 10.16352/j.issn.1001-6325.2022.12.1855

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Toll-like receptor 3 activation combined with INFα inhibits the expression of DISC1 in hippocampal neurons of mice

YAO Jing-hong¹, LI Jia-huan¹, LIU Zi-jian^2*

1. Department of Infectious Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022;
2. Department of Anatomy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China

Received:2021-11-08 Revised:2022-04-19 Online:2022-12-05 Published:2022-11-23
Contact: ^* liuzj@mail.hust.edu.cn

Abstract

Abstract: Objective To explore the effect of Toll-like receptor 3 (TLR3) activation on the expression of neuronal disrupted-in-schizophrenia 1 (DISC1). Methods C57BL/6J mouse hippocampal neurons were incubated with polyI:C(1 μg/mL) or/and mIFNα(100 IU/mL) for 24 hrs. DISC1 mRNA and protein were detected by quantitive RT-PCR, immuno-cytochemistry staining and Western blot. The expression of STAT1, phospho-STAT1, p38 MAPK, phospho-p38 MAPK, GSK-3β, and phospho-GSK-3β was also detected by Western blot. Results Comparing with control group, the mRNA and protein expression of DISC1 in polyI:C group and IFNα group didn't show any changes, but significantly decreased with the co-stimulation by polyI:C and IFNα (P＜0.05). In addition, the phosphorylation of STAT1 was significantly increased in both IFNα group and co-stimulation group(P＜0.05) and the phosphorylation of p38 MAPK was elevated in polyI:C group and co-stimulation group (P＜0.05).The expression of GSK-3β and phospho-GSK-3β had no changes. Conclusions The combined stimulations of IFNα and TLR3 activation may inhibit the expression of DISC1 potentially through JAK/STAT1 and p38 MAPK signaling pathways.

Key words: disrupted-in-schizophrenia 1, Toll-like receptor 3, interferon α, STAT1, p38 MAPK

CLC Number:

R373.2

YAO Jing-hong, LI Jia-huan, LIU Zi-jian. Toll-like receptor 3 activation combined with INFα inhibits the expression of DISC1 in hippocampal neurons of mice[J]. Basic & Clinical Medicine, 2022, 42(12): 1855-1860.

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Toll-like receptor 3 activation combined with INFα inhibits the expression of DISC1 in hippocampal neurons of mice

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