Abstract: Objective To investigate the effect of recombinant Newcastle disease virus expressing IL-29(rL-IL29) on the proliferation and migration with invasion of gastric cancer cells and to explore the potential mechanism. Methods Gastric cancer cell lines BGC were cultured and divided into the PBS, rL-IL29 and newcastle disease virus(NDV)groups infected by the PBS, rL-IL29 or NDV.Western blot was used to detecte the expression of NDV, IL-29,p-ERK,MMP2 and p-AKT protein in cells.The proliferation,migration and invasion of gastric cancer cell lines BGC were detected by CCK8、cell clone forming experiments、scratch test and Transwell invasion test. Results IL-29 protein was only expressed in rL-IL29 group,NDV protein were expressed both in rL-IL29 group and NDV group and was significantly higher than those of PBS group (P＜0.05). The proliferation and migration of BGC cells infected with rL-IL29 were inhibited more seriously than those infected with NDV or PBS group.The expression of p-ERK,MMP2 (66 ku/72 ku),p-AKT and P65 protein was all significantly decreased(P＜0.05). Conclusions The proliferation and migration of gastric cancer cell lines BGC were inhibited by rL-IL29 and the mechanism may be related with ERK,AKT signaling pathways.

Key words: recombinant Newcastle disease virus, IL-29, gastric cancer cell line BGC, proliferation, migration