Abstract: Objective To investigate the effect of phosphatase and tensin homolog deleted on chromosome 10(PTEN) on the apoptosis of H9C2 cardiomyocytes induced by H2O2. Methods H9C2 cardiomyocytes were divided into control group (normal cultured cells), H2O2 group (100 μmol/L H2O2 treatment for 24 h), propofol (5, 10, 30 μmol/L) group were treated with H2O2 for 24 h and then pretreated with propofol of different concentrations (5, 10, 30 μmol/L) for 1 h. The viability of cells was detected by MTT assay and the apoptosis rate was measured by flow cytometry. The expression of PTEN was detected by RT-qPCR; The viability, apoptosis, lactate dehydrogenase (LDH) , and reactive oxygen species (ROS) were analyzed of overexpression of PTEN. Results Compared with 0 μmol/L, 5 μmol/L and 10 μmol/L propofol significantly increased the activity of cardiomyocytes induced by H2O2, decreased the rate of apoptosis, and inhibited the expression of PTEN (P<0.05); 30 μmol/L propofol significantly inhibited cell viability and promoted apoptosis and the expression of PTEN (P<0.05); overexpression of PTEN could inhibit the protective effect of propofol against H2O2-induced H9C2 cells (P<0.05), and promote the expression of LDH and ROS (P<0.05). Conclusion PTEN inhibits the protective effect of propofol against H2O2-induced apoptosis of cardiomyocytes.

Key words: H9C2 cardiomyocytes, Propofol, LDH, PTEN