Abstract: Objective To investigate the effect of miR-34a on the invasion and metastasis of glioma cells by regulating the expression of matrix metalloproteinase 2 (MMP2), which is a target gene of miR-34a.Methods Compared with human normal brain glial cells (HEB),U87 and A172 with MMP2 high expression were selected to transfect miR-34a mimics and miR-34a inhibitors.To observe the expression of miR-34a and MMP2 in the multiple glioma cell lines by RT-qPCR and western blot.After predicting the regional of miR-34a and MMP2 with microRNA database, construct MMP2 3'UTR wild-type and mutant luciferase plasmids and miR-34a plasmids, to observe the wild type and mutant luciferase activity changes by the luciferase reporter gene experiments. The invasion and migration abilities of U87 in blank control group, MMP2 group and miR-34a /MMP2 group were measured by Transwell and wound healing assay. Results To select U87 and A172 , MMP2 protein and mRNA levels were decreased after over-expression of miR-34a, and were increased after decreasing miR-34a,compared with the normal group, the p value of protein and mRNA was less than 0.05; miR-34a combined with MMP2 mRNA 3 'UTR region to reduce the MMP2 the level of mRNA, miR-34a inhibited cell migration and invasion by down-regulating MMP2.Conclusion miR-34a can down-regulate the invasion and migration ability of MMP2 in glioma cells.

Key words: Keywords: miR-34a, matrix metalloproteinase 2(MMP2), glioma, invasion and migration