Abstract: Objective To probe the interaction between TGF-βRI and Fascin1. Methods The co-localizaion of TGF-βRI and Fascin1 was verified by immunofluorescence. Meanwhile, GST pull-down and Co-IP assay were used to identify the interaction and the binding site between TGF-βRI and Fascin1. Results The immunofluorescence experiment showed that the two proteins localized at the same places. TGF-βRI was accociated with Fascin1. And the N-terminal of Fasicn1 and ICD domain of TGF-βRI were invovled in the interacton. Conclusion TGF-βRI interacts with Fascin1, and the N-terminal of Fasicn1 and ICD domain of TGF-βRI were invovled in the interacton.

Key words: Keywords: TGF-β1, TβR?, Fascin1, Interaction