Abstract: Objective: We observed actions and effects of anthocyanins on antiproliferation and proapoptosis in human colon cancer cells LS174T, making further investigation of anti-cancer mechanism of blueberry anthocyanin, accordingly, those provide the experimental basis for the prevention and therapy of colon cancer in clinic. Methods: Colon cancer cells ls174t were cultured in vitro,treating LS174T cells with blueberry anthocyanins (25、50、100 and 200μg/mL).We tested the effects of blueberry anthocyanins on proliferation and apoptosis by CCK and Hoechst 33258 chromatin staining dye; We analyzed mRNA expression of apoptosis-related genes(including p53、ccnd3、p73、sfn、caspase8 and fam200a) by real-time PCR. In addition, protein levels of caspase8 and p73 were also analyzed using western blot. Results: The blueberry anthocyanins suppressed the proliferation and induced the apoptosis of LS174T cells respectively in a dose-dependent and time-dependent manners; the expression of ccnd3、p73 and sfn mRNA were up-regulated while the expression of caspase8、fam200a and p53 mRNA were down-regulated; the expression of protein levels of caspase8 and p73 were down-regulated and up-regulated respectively. Conclusion: Blueberry anthocyanins induce cell cycle arrest and apoptosis of colon cancer LS174T cells, which were associated with an up-regulation of the expression of tumor suppressor p73 and a down-regulation of the expression of caspase8.

Key words: Key words: anthocyanins, anticancer, colon cancer, blueberry