Abstract: Objective To investigate the effect of acetylpuerarin on expression of caspase-8 andcaspase-3 in BV-2 mousemicrogliacell line induced by Aβ25-35. Methods BV-2 microglia cellswere divided into six groups: control group,model group(cells were activated with condensed Aβ25-35 as cell model),acetylpuerarininterventiongroups (low-dose group,moderate-dose group, high-dose group)andcaspase-3 inhibitor (Z-IETD-fmk) group. The protein expressionof caspase-8 andcaspase-3 was detected by Westernblot. ThemRNA expressionof caspase-8 andcaspase-3 was measured by real-time PCR. ResultsCompared with control group, protein expression ofcaspase-8 and caspase-3 in model groupwas significantly up-regulated (P<0.01), and gene expression ofcaspase-8 and caspase-3was markedlyincreased (P<0.01). Compared withmodel group,protein expressionof caspase-8 andcaspase-3in acetylpuerarin each dose groupwas significantly down-regulatedin different degrees (P<0.01),and gene expressionof caspase-8 andcaspase-3 was markedlydecreased(P<0.01).ConclusionsAcetylpuerarin can inhibit caspase-8 andcaspase-3expression ofBV-2 mouse microglia cell line induced by Aβ25-35in a dose-dependent manner.

Key words: acetylpuerarin, Alzheimer’s disease, β-amyloid protein, microglia, caspase