Abstract: Objective To investigate the expression levels of ANKRD49 in non-small cell lung cancer (NSCLS) and its clinical significance. Methods The specimens of carcinoma tissues and their paired para-carcinoma tissues were collected from 75 NSCLS patients and were made into tissue microarray. Immunohistochemistry was applied to detect the expression level of ANKRD49 in these specimens. The correlation between the expression level of ANKRD49 and the different clinical parameters of NSCLC was analyzed. Western blot assay was used to detect the expression level of ANKRD49 in human airway epithelial cell line (HBEC) and different lung cancer cell lines. Results The positive expression rate of ANKRD49 in the carcinoma tissues （82.67%）was significantly higher than that in the para-carcinoma tissues （49.33%）（P ＜ 0. 05）, and the positive expression rate of ANKRD49 in poorly differentiated carcinoma tissues （80%）was significantly higher than that in well differentiated carcinoma tissues （42.6%）（P ＜ 0. 05）. ANKRD49 was expressed only in A549 and NCI-H1650 lung adenocarcinoma cell lines rather than in normal airway epithelial cell line and other lung cancer cell lines. Conclusion ANKRD49 may be involved in the progression of NSCLC.

Key words: ANKRD49, non-small cell lung cancer, tissue chip, cancer /testis antigen