Abstract: Objective To investigate the effect of CCK-8 on CHOP/caspase-11/caspase-1 expressions by LPS in RAW264. 7 cell. Methods RAW264. 7 cells were incubated with LPS and/or CCK-8 and/or CCK-8 1R blocking agent devazepide for indicated times. CHOP and caspase-11 protein expressions were determined by western blot, caspase-1 activity were analyzed by kit, IL-1β expression levels were analyzed by ELISA. Results Both CHOP and caspase-11 expression were upregulated in LPS-activated RAW264. 7 cell, which were inhibited by pre-treatment of CCK-8. Pre-treatment of devazepide inhibited the effects of CCK-8 significantly.The same results were seen in caspase-1 activity and IL-1β expression. Conclusion CCK-8 exerts an anti-inflammatory effect by inhibiting CHOP/caspase-11/caspase-1 expressions induced by LPS in RAW264. 7 cell. CCK 1R might be responsible for the anti- inflammatory effect of CCK-8.

Key words: cholecystokinin, lipopolysaccharides, C/EBP homologous protein, caspase-11, caspase-1