Abstract: The P53 is a key tumor suppressor. Many studies have shown that P53 plays an important role in inducing ESCs differentiation. P53 binding with P21, which is one of transcriptional targets of the P53, and knockdown of nucleolin induce cell cycle arrest, G1 phase accumulation and differentiation in ESCs by up-regulating P53 protein level; Moreover, HIF2α high expression inhibiting P53 activity and promoting Aurora Kinase-P53 Signaling can maintain the “stemness” and inhibit differentiation.

Key words: ESCs, P53, P21, HIF-2a, Aurka