Abstract: Objective To investigate the expressions of vascular endothelial growth factor、NF-κB and MMP-9 in NB4 cell lines affected by VPA in combination with arsenic trioxide. Methods The experimental included control group、the group of arsenic trioxide、the group of VPA and the group of arsenic trioxide combined with VPA, MTT assay method was used to detect the cell survival rate. RT-PCR was used to detect the mRNA level of VEGF、NF-κB and MMP-9.Western Blot was used to detect the protein level of VEGF、NF-κB and MMP-9. Results VPA can increase the decreased survival rate induced by arsenic trioxide in NB4 cell lines, the expression of VEGF、NF-κB and MMP-9 in NB4 cell lines were increased under the effect of arsenic trioxide (P<0.05). The expression of VEGF、NF-κB and MMP-9 in the group of VPA combined with arsenic trioxide were all decreased than in the group of arsenic trioxide alone(P＜0.05).Conclusion VPA can down-regulate the increased expression of VEGF、NF-κB and MMP-9 caused by arsenic trioxide.

Key words: arsenic trioxide, VEGF, NF-κB, MMP-9, NB4 cell line