Abstract: Objective To explore the difference of Hans, Choi immunophenotyping and c-myc gene characteristics between pediatric and adult diffuse large B-cell lymphoma. Methods To Collect 60 cases of DLBCL clinicopathological data and follow-up, 17 cases of children group, adult group of 43 patients. To observe CD10, bcl-6, MUM1, FOXP1, GCET1 and CD5 protein expression by immunohistochemical SP method and accord to Hans and Choi immunophenotyping standard genotyping. To detect c-myc gene circumstances Interphase fluorescence insituhybridization (FISH) . Results 1) Hans typing：the Children DLBCL group of GCB were 11 cases, non-GCB type were 6 cases； the GCB type in the adult DLBCL group were nine cases, non-GCB type were 34 cases. 2) Choi typing：the Children DLBCL group of GCB type were 13 cases, ABC type were 4 cases； the adult DLBCL group of GCB type were 13 cases, ABC type were 30 cases (P = 0.008). 3) c-myc gene：The c-myc gene disruption of children DLBCL were 5 cases; c-myc gene in 43 adult cases were normal(P=0.006). Conclusions Child DLBCL are mainly GCB-type with better prognosis；but non-GCB-type are more common type in adult with worse prognosis.The c-myc gene disruption in Children DLBCL was significantly higher than that of adult ones.

Key words: Child, lymphoma, Immunophenotyping, Insituhybridization