Abstract: Objective The application of WCX magnetic beads combined with MALDI-TOF MS in detecting differentially expressed proteins in serum of renal angiomyolipoma can reduce the misdiagnosis rate. Methods WCX beads combined with MALDI-TOF MS technique in detecting differentially expressed proteins in serum of renal angiomyolipoma can screen out differentially expressed proteins. And genetic algorithm was utilized to establish a diagnosis model. Results The technique screened out 142 different protein, and 13 were significantly different (P<0.05). ClinProTools2.2 software was utilized with genetic algorithm to screen out 15 differentially expressed proteins to establish a diagnosis model. The recognition rate was 100%. Conclusions The iagnosis model established by the application of WCX magnetic beads combined with MALDI-TOF MS can reduce the misdiagnosis rate of renal angiomyolipoma.

Key words: renal angiomyolipoma, proteomics, bead, serum, diagnosis