Abstract: Objective To detect the expression of Annexin Ⅴ in hypoxic trophoblast cells including in vivo and in vitro and explore the signifiance. Methods The expression of annexin Ⅴin hypoxic trophoblast cells was detected by hypoxic cell culture, immunohistochemistry method, flow cytometry, and its effects on blood coagulation. Results (1) The expression of AnnexinⅤ decreased in trophoblast cell culture in vitro in hypoxia. (2) The expression of AnnexinⅤ also decreased in trophoblast cells in vivo in hypoxia (preeclampsia placenta).(3) With the increase of the concentration of Annexin Ⅴ, peripheral coagulation markers were observed clinical variation in vitro. Conclusion The expression of Annexin Ⅴin trophoblast cells in hypoxic was significantly reduced, which may affect placental function, and lead to a variety of adverse pregnancy outcomes.

Key words: hypoxia, Annexin Ⅴ, coagulation, trophoblast cells