Esketamine reduces ischemia-reperfusion injury of skeletal muscle of rats

doi:10.16352/j.issn.1001-6325.2023.12.1822

Abstract

Abstract: Objective To investigate the role of low dose esketamine in pretreated limb ischemia-reperfusion injury. Methods The rats were divided into sham-operated group (Sham group), ischemia-reperfusion group (I/R group, 3 h of ischemia and 2 h of reperfusion), esketamine group (ESK group, ip, 5 mg/kg). The plasma concentrations of creatine kinase (CK) and lactate dehydrogenase (LDH) were measured. The wet/dry weight ratio of skeletal muscle was immediately detected. The gastrocnemius muscle was harvested and the level of malondialdehyde (MDA) and superoxide dismutase (SOD) was detected by colorimetric assay. The expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) were detected by Western blot and immunohistochemical staining. Results Compared with Sham group, the index of wet/dry weight ratio, MDA, CK, LDH, Nrf2 and HO-1 were all increased, but SOD was decreased in I/R group(P＜0.05). Compared with I/R group, the index of wet/dry weight ratio, MDA, CK and LDH were significantly lower, but SOD and Nrf2 and HO-1 were significantly higher in ESK group (P＜0.05). Conclusions ESK may increase Nrf2 in the nucleus, thereby increase the HO-1 protein as an antioxidant agent.

Key words: esketamine, ischemia-reperfusion injury, oxidative stress, Nrf2

CLC Number:

R364

YUAN Peigen, CHEN Shunli, SHAN Yuanlu, XUE Binbin, YE Yuzhu, LIN Lina. Esketamine reduces ischemia-reperfusion injury of skeletal muscle of rats[J]. Basic & Clinical Medicine, 2023, 43(12): 1822-1826.

References 11

[1]	Mas E, Anne E, Corcoran TB, et al. Effects of spinal or general anesthesia on F(2)-isoprostanes and isofurans during ischemia/reperfusion of the leg in patients undergoing knee replacement surgery[J]. Free Radic Biol & Med, 2011, 50: 1171-1176.
[2]	Jinwei L, Shanhu W, Wenxi X, et al. Ketamine ameliorates oxidative stress-induced apoptosis in experimental traumatic brain injury via the nrf2 pathway[J]. Drug Des Devel Ther, 2018, 4: 845-853.
[3]	Shilin X, Ying Z, Qianyu W, et al. Ketamine attenuates airway inflammation via inducing inflammatory cells apoptosis and activating Nrf2 pathway in a mixed-granulocytic murine asthma model[J]. Drug Des Devel Ther, 2022, 12: 4411-4428.
[4]	Yanbin T, Yufang L, Huanzhu Z, et al. Esketamine is neuroprotective against traumatic brain injury through its modulation of autophagy and oxidative stress via AMPK/mTOR-dependent TFEB nuclear translocation[J]. Exp Neurol, 2023, 366: 114436. doi:10.1016/j.expneurol.2023.114436.
[5]	Buffin MB, Pierre AC, Delage C, et al. EGF receptor transactivation and PI3-kinase mediate stimulation of ERK by α2Aadrenoreceptor in intestinal epithelial cells: a role in wound healing[J]. Eur J Pharmacol, 2007, 574: 85-93.
[6]	王琼, 董倩兰, 朱燕亭, 等. 桔梗皂苷D减轻大鼠肾缺血/再灌注损伤[J]. 基础医学与临床, 2023, 43: 1222-1228.
[7]	Avci G, Kadioglu H, Sehirli AO, et al. Curcumin protects against ischemia/reperfusion injury in rat skeletal muscle[J]. J Surg Res, 2012, 172: e39-e46.
[8]	Kwark MK, Wakabayashi N, Kensler TW. Chemoprevention through the Keap1-Nrf2 signaling pathway by phase 2 enzyme inducers[J]. Mutal Res, 2004, 555: 133-148.
[9]	袁培根, 薛彬彬, 林碧, 等. Nrf2/ARE通路介导右美托咪定减轻肢体缺血/再灌注损伤中的作用[J]. 中国应用生理学杂志. 2016, 32: 250-254.
[10]	Mcmahon M, Itoh K, Yamamoto M, et al. Keap1-dependent proteasomal degradation of transcription factor Nrf2 contributes to the negative regulation of antioxidant response element-driven gene expression[J]. J Biol Chem, 2003, 278: 21592-21600.
[11]	陈鹤翔. 艾司氯胺酮在脓毒症心肌损伤中的保护作用及机制研究[D].武汉: 武汉大学, 2021: 1-89.

[1]	ZHANG Xiangxin, LI Wen. Mitophagy in hepatic ischemia-reperfusion injury [J]. Basic & Clinical Medicine, 2024, 44(6): 877-881.
[2]	YANG Hanyi, NING Jiayi, WANG Xiaolan, ZHANG Yimeng, XIE Tingke, CHEN Yixuan, HAN Jing. Melatonin inhibits H₂O₂-induced oxidative stress and apoptosis marker protein expression in human umbilical vein endothelial cells [J]. Basic & Clinical Medicine, 2024, 44(5): 645-650.
[3]	WANG Rui, LI Rui, LI Yunlong, ZHANG Yuping, YU Kang, LIU Yanping. Research progress of strontium and pregnancy and pregnancy diseases [J]. Basic & Clinical Medicine, 2024, 44(4): 428-433.
[4]	ZHANG Weiwei, XIE Jing, LI Lihua. Paricalcitol inhibition of oxidative stress alleviates the damage of hepatocyte tight junction in mice [J]. Basic & Clinical Medicine, 2024, 44(3): 317-324.
[5]	ZHANG Xiuli, HU Xiaogang, MO Jun, CHEN Ling. Guanxinning tablet alleviates carotid atherosclerotic plaque injury in rats [J]. Basic & Clinical Medicine, 2024, 44(2): 192-198.
[6]	MU Yang, YANG Zhiwen. Dexmedetomidine alleviates TNF-α-induced injury of human neuroblastoma cell line SH-SY5Y through activating AMPK [J]. Basic & Clinical Medicine, 2024, 44(2): 147-153.
[7]	. Role of TERT in regulating mitochondrial oxidative stress in diseases [J]. Basic & Clinical Medicine, 2024, 44(10): 1436-1441.
[8]	ZHANG Weicai, LIU Siren, WANG Shangnong. Sodium hydrosulfide increases glutathione synthesis in cardiac muscle cell line HL-1 under hyperglycemia and hyperlipidemia [J]. Basic & Clinical Medicine, 2024, 44(1): 69-76.
[9]	CHEN Xinjun, WANG Qinyu, CHEN Le, GU Chunyu, WU Zhuo. Pterostilbene reduces paraquat-induced pulmonary fibrosis in rat model [J]. Basic & Clinical Medicine, 2023, 43(9): 1383-1389.
[10]	WANG Qiong, DONG Qianlan, ZHU Yanting, JIN Gang, ZHANG Linping. Platycodon D attenuates renal ischemia-reperfusion injury in rats [J]. Basic & Clinical Medicine, 2023, 43(8): 1222-1228.
[11]	YANG Weizhu, LIU Zhong, ZHANG Fenglin, HUANG Yanlin, SUN Jin, TIAN Xinhua. NOX4-mediated oxidative stress is involved in the occurrence of pituitary adenoma hemorrhagic stroke [J]. Basic & Clinical Medicine, 2023, 43(7): 1040-1044.
[12]	LU Yapeng, XIE Jianqin. Research progress on the role of NLRP3 inflammasome in the treatment of ischemia-reperfusion injury [J]. Basic & Clinical Medicine, 2023, 43(4): 706-710.
[13]	WANG Bo, ZHANG Minghuan. CIRC_0044235 targeting miR-574-5p attenuates IL-1β-induced injury in human chondrocyte [J]. Basic & Clinical Medicine, 2023, 43(3): 419-426.
[14]	WANG Minrong, ZHANG Feng, LU Liqun. Construction and genotype identification of Nrf2 knockout mouse model [J]. Basic & Clinical Medicine, 2023, 43(3): 415-418.
[15]	ZHANG Mengyao, NIU Shu, CAI Jing, ZHANG Lixiang, ZHAO Zhigang. Tanshinone ⅡA attenuates high glucose-induced apoptosis and oxidative stress in mouse podocyte line PMC-5 [J]. Basic & Clinical Medicine, 2023, 43(2): 277-282.