HMGB1 attenuates the mitigative effect to myocardial ischemia-reperfusion injury by tanshinone ⅡA in rats

doi:10.16352/j.issn.1001-6325.2022.09.1333

Abstract

Abstract: Objective To study the influence of exogenous high-mobility group box 1(HMGB1) on the mitigative effect of tanshinone ⅡA (TSA) to myocardial ischemia-reperfusion (I/R) injury. Methods Rats were divided into sham, I/R (ligation of left anterior descending coronary artery), TSA (I/R + TSA intravenous injection) and TSA+HMGB1 (I/R+TSA intravenous injection + recombination HMGB1 intraperitoneal injection) groups. Serum CK-MB was measured by ELISA. Myocardial infarct area was measured by TCC. The expression of inflammatory indicators were measured by RT-qPCR and Western blot. Results Compared with sham group, CK-MB, myocardial infarct area, transcription level of IL-6, IL-1β and TNF-α, expression of HMGB1, NLRP3 and caspase-1 all increased in I/R group(P＜0.05). Compared with I/R group, CK-MB, myocardial infarct area, transcription levels of IL-6, IL-1β and TNF-α, expressions of HMGB1, NLRP3, caspase-1 and NF-κB decreased in TSA group (P＜0.05). However, compared with TSA group, CK-MB, myocardial infarct area, transcription levels of IL-6, IL-1β and TNF-α, expressions of HMGB1, NLRP3, caspase-1 and NF-κB increased in TSA+HMGB1 group(P＜0.05). Conclusions Exogenous HMGB1 attenuates the mitigative effect of tanshinone ⅡA to rat myocardial I/R injury through up-regulation the expressions of inflammatory factors.

Key words: high-mobility group box 1, tanshinone ⅡA, myocardial ischemia-reperfusion injury, inflammatory factor

CLC Number:

R543.3

ZHONG Jin-peng, YANG Li, WANG Hui-bo,CHEN Hong-jian, LI Jin-wei, WEN Ming-hong, LYU Yun-bo. HMGB1 attenuates the mitigative effect to myocardial ischemia-reperfusion injury by tanshinone ⅡA in rats[J]. Basic & Clinical Medicine, 2022, 42(9): 1333-1338.

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