Effect of PTOV1 expression on prognosis of colorectal cancer patients

doi:10.16352/j.issn.1001-6325.2022.08.1176

Abstract

Abstract: Objective To analyze the effect of over-expression of prostate tumor 1 (PTOV1) on the prognosis of colorectal cancer (CRC) based on bioinformatics, and to demonstrate the effect of PTOV1 on the proliferation of CRC cells. Methods Combined with bioinformatics and biological experiments, PTOV1 expression in CRC tissues and cell lines was analyzed. PTOV1 expression was confirmed by Western blot and RT-qPCR. The effect of PTOV1 on the proliferation of CRC cells was investigated by colony formation assay and EdU assay after transfection by PTOV1 siRNA in SW480 cell line. Results PTOV1 expression was significantly up-regulated in CRC tissues and cell lines (P＜0.05). More biological experiments results showed that PTOV1 as an oncogene was associated with poor prognosis of CRC. In vitro experiments showed that PTOV1 knockdown inhibited the proliferation of SW480 cells(P＜0.05). Conclusions PTOV1 is highly expressed in CRC and is associated with poor prognosis in CRC patients. PTOV1 is a potential prognostic biomarker for the progression of CRC.

Key words: colorectal cancer, PTOV1, prognosis, biomarkers, proliferation

CLC Number:

R735.7

XIE Si-an, CHEN Yi-yang, XU Jun-xuan, NING Ting-ting, ZHANG Nan, ZHU Sheng-tao, ZHANG Shu-tian. Effect of PTOV1 expression on prognosis of colorectal cancer patients[J]. Basic & Clinical Medicine, 2022, 42(8): 1176-1181.

References 12

[1]	Tsukamoto S, Ishikawa T, Iida S, et al. Clinical significance of osteoprotegerin expression in human colorectal cancer[J]. Clin Cancer Res, 2011, 17:2444-2450.
[2]	Sung H, Ferlay J, Siegel RL, et al. Global cancer statistics 2020:globocan estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin, 2021, 71:209-249.
[3]	Benedit P, Paciucci R, Thomson TM, et al. PTOV1, a novel protein overexpressed in prostate cancer containing a new class of protein homology blocks[J]. Oncogene, 2001, 20:1455-1464.
[4]	Lei F, Zhang L, Li X, et al. Overexpression of prostate tumor overexpressed 1 correlates with tumor progression and predicts poor prognosis in breast cancer[J]. BMC Cancer, 2014, 14:457. doi:10.1186/1471-2407-14-457.
[5]	Sinicrope FA. Increasing incidence of early-onset colorectal cancer[J]. N Engl J Med, 2022, 386:1547-1558.
[6]	Shen H, Liao B, Wan Z, et al. PTOV1 promotes cisplatin-induced chemotherapy resistance by activating the nuclear factor kappa B pathway in ovarian cancer[J]. Mol Ther Oncolytics, 2021, 20:499-507.
[7]	Wu Z, Liu Z, Jiang X, et al. Depleting PTOV1 sensitizes non-small cell lung cancer cells to chemotherapy through attenuating cancer stem cell traits[J]. J Exp Clin Cancer Res, 2019, 38:341. doi:10.1186/s13046-019-1349-y.
[8]	Karna SKL, Ahmad F, Lone BA, et al. Knockdown of PTOV1 and PIN1 exhibit common phenotypic anti-cancer effects in MDA-MB-231 cells[J]. PLoS One, 2019, 14:e0211658. doi:10.1371/journal.pone.0211658.
[9]	Morote J, Fernandez S, Alana L, et al. PTOV1 expres-sion predicts prostate cancer in men with isolated high-grade prostatic intraepithelial neoplasia in needle biopsy[J]. Clin Cancer Res, 2008, 14:2617-2622. doi:10.1158/1078-0432.CCR-07-4987.
[10]	Pennington KL, McEwan CM, Woods J, et al. SGK2, 14-3-3, and HUWE1 cooperate to control the localization, stability, and function of the oncoprotein PTOV1[J]. Mol Cancer Res, 2022, 20:231-243.
[11]	Santamaria A, Castellanos E, Gomez V, et al. PTOV1 enables the nuclear translocation and mitogenic activity of flotillin-1, a major protein of lipid rafts[J]. Mol Cell Biol, 2005, 25:1900-1911.
[12]	Marques N, Sese M, Canovas V, et al. Regulation of protein translation and c-Jun expression by prostate tumor overexpressed 1[J]. Oncogene, 2014, 33:1124-1134.

[1]	XIAO Yanhong, JIANG Mingdong, LIN Yeyuan, RAN Can, LIANG Bo. Scutellarin inhibits proliferation and migration of human prostate cancer cell line PC-3 [J]. Basic & Clinical Medicine, 2024, 44(9): 1229-1235.
[2]	LI Shunle, LI Rong, TIAN Yaya, RAN Xiaoli, ZHAO Yanpei, XU Meng. Association of serum KLF5 and actinin-4 levels with prognosis of TACE treatment for hepatocellular carcinoma [J]. Basic & Clinical Medicine, 2024, 44(9): 1284-1289.
[3]	HOU Nan, LIU Yuan, GAO Jun, WANG Jing, YUAN Meng. Whole blood NPM1, MCP-1 and intestinal flora are associated with gastric cancer progression and prognosis [J]. Basic & Clinical Medicine, 2024, 44(8): 1137-1142.
[4]	YAO Anjun, CHEN Lingzi, JIN Huixian. Docosahexaenoic acid inhibits proliferation of human colon cancer cell line HT-29 [J]. Basic & Clinical Medicine, 2024, 44(8): 1107-1112.
[5]	ZHENG Hong, CHEN Xiaoxia, HAN Lizhou, CHEN Miao, HUANGFU Juan. Decrease of lncRNA-RMRP expression inhibits proliferation and invasion of human lung cancer cell line A549 [J]. Basic & Clinical Medicine, 2024, 44(7): 974-978.
[6]	CAO Qingwen, QI Lin, YU Bo, TIAN Chenchen, YUAN Haining, WANG Yue. Salidroside promotes proliferation and migration of human vascular endothelial cell line EA.hy926 [J]. Basic & Clinical Medicine, 2024, 44(7): 925-930.
[7]	ZHANG Pengju, LI Jie, ZHANG Mengdi, SUN Shaoke, XU Yinzhe. KAT8 promotes the proliferation of colorectal cancer cell lines by enhancing METTL3 expression [J]. Basic & Clinical Medicine, 2024, 44(7): 931-939.
[8]	GAO Yinjie, TONG Anli. Mechanisms of cell death and proliferation of aldosterone-producing adenoma [J]. Basic & Clinical Medicine, 2024, 44(6): 758-762.
[9]	CAO Chenxin, TANG Hui, GENG Ruixuan, GUO Fuping, BAI Chunmei, WANG Yingyi, LI Taisheng. Correlation between circulating CD4⁺CD45RA⁺CD62L⁺ T cells and prognosis of metastatic non-small cell lung cancer treated with EGFR-TKI [J]. Basic & Clinical Medicine, 2024, 44(5): 658-664.
[10]	WANG Tingting, XIAO Ning, LIN Dianxin, DONG Haitao. Allicin inhibits the proliferation of oral cancer cells line CAL27 [J]. Basic & Clinical Medicine, 2024, 44(5): 673-676.
[11]	JIA Anna, GUO Jinxin, ZHANG Xuan, ZHAN Shijia, YU Yongbo, GUO Yongli, CHANG Yan. Regulation of TMEFF1 on proliferation of neuroblastoma cell lines [J]. Basic & Clinical Medicine, 2024, 44(5): 637-644.
[12]	LI Yunlong, LI Rui, ZHANG Yuping, WANG Rui, LIU Yanping. Zinc deficiency during pregnancy and its effect on pregnancy outcome [J]. Basic & Clinical Medicine, 2024, 44(4): 422-427.
[13]	LIANG Xiangcun, WEI Xiaoyu, LIANG Jian, WANG Qing, GENG Guang. Anlotinib inhibits proliferation and promotes apoptosis of human non-small cell lung cancer cell lines through miR-16-5p/PD-1 axis [J]. Basic & Clinical Medicine, 2024, 44(4): 503-512.
[14]	LU Kai, LU Jianju, GUO Wenli, HUANG Jianqi, LI Zhihua. lncRNA VIM-AS5 expression and its effect on proliferation and migration of human breast cancer cell lines [J]. Basic & Clinical Medicine, 2024, 44(4): 447-453.
[15]	GUO Hongjiang, XU Yeting, ZHANG Diya, QIU Jiaxing, WANG Yucheng, JU Rui, GUO Lei. Effect of carboxyamidotriazole-orotate on proliferation and fatty acid anabolism of human pancreatic cancer cell lines [J]. Basic & Clinical Medicine, 2024, 44(4): 440-446.