mRNA差异显示法在维吾尔族和哈萨克族2型糖尿病患者白细胞差异基因筛选中的应用

基础医学与临床 ›› 2008, Vol. 28 ›› Issue (4): 344-348.

mRNA差异显示法在维吾尔族和哈萨克族2型糖尿病患者白细胞差异基因筛选中的应用

邹林樾张朝霞张琼魏琴卓菲娅·克依木

新疆医科大学第一附属医院实验动物科学研究中心新疆医科大学第一附属医院检验科新疆医科大学第一附属医院检验科新疆医科大学第一附属医院实验动物科学研究中心新疆医科大学第一附属医院实验动物科学研究中心

收稿日期:2007-03-15 修回日期:2005-06-14 出版日期:2008-04-25 发布日期:2008-04-25
通讯作者: 张朝霞

Application mRNA differential display in screening differential expressed genes of peripheral blood leucocyte of Uigur and Kazak patients with type 2 diabetes mellitus

Lin-yue ZOU, Zhao-xia ZHANG, Qiong ZHANG, Qin WEI, Zhuo-feiya

Animal Research Central,The First Affiliated Hospital of Xingjiang Medical University Clinical Laboratory,The First Affiliated Hospital of Xingjiang Medical University

Received:2007-03-15 Revised:2005-06-14 Online:2008-04-25 Published:2008-04-25
Contact: Zhao-xia ZHANG,

摘要/Abstract

摘要： 目的:选择、研究三代内未和其他民族通婚的维吾尔族2型糖尿病人与维吾尔族正常人，哈萨克族2型糖尿病人与哈萨克族正常人之间外周血白细胞mRNA差异表达，寻找致糖尿病基因和抑糖尿病基因。方法：应用荧光mRNA差异显示技术分析、鉴定外周血白细胞的差异表达片段，测序后进行同源性分析。结果：发现Z5、Z8、Z15 3个差异表达明显的cDNA片段在正常人中高表达，而在2型糖尿病人中低表达或不表达。其中Z5、Z8与CREG1基因同源，Z15基因未知。结论：发现3个在两个民族正常人高表达，而病人缺乏或低表达的基因，可能与抑糖尿病基因有关，可能是2型糖尿病的病因之一。

Abstract: Objective To search the differentially expressed genes in Uigur and Kazak patients with and without type 2 diabetes mellitus. Methods The differentially expressed cDNA bands were isolated by fluorescent mRNA differential display from peripheral blood leucocyte of the Uigur and Kazak patients with type 2 diabetes mellitus and the normal controls. After being cloned, all cDNA fragments were sequenced, then underwent sequence analysis, homogenous comparison，and Northern blot analysis. Result Z5、Z8、Z15 differentially expressed cDNA fragments were found.They were over-expressed in the normal controls and were lower or scarced in the Uigur and Kazak patients with type 2 diabetes mellitus. They were selected for sequencing and hybridization. Z5、Z8 showed highly homologous to cellular repressor of E1A-stimulated genes，Z15 are unknown. Conclusion The three differentially expressed genes might have a relation with type 2 diabetes mellitus.

邹林樾张朝霞张琼魏琴卓菲娅·克依木. mRNA差异显示法在维吾尔族和哈萨克族2型糖尿病患者白细胞差异基因筛选中的应用[J]. 基础医学与临床, 2008, 28(4): 344-348.

Lin-yue ZOU; Zhao-xia ZHANG; Qiong ZHANG; Qin WEI; Zhuo-feiya. Application mRNA differential display in screening differential expressed genes of peripheral blood leucocyte of Uigur and Kazak patients with type 2 diabetes mellitus[J]. Basic & Clinical Medicine, 2008, 28(4): 344-348.