关键词: 人急性髓性白血病, 白杨素, 8－硝基白杨素, 细胞凋亡

Abstract: Objective To investigate the effect of 8-nitrochyrsin on cell proliferation and cell apoptosis of HL-60 cells in vitro. Methods 8-nitrochyrsin on proliferation of HL-60 cells was detected by MTT assay; The trypan blue exclusion method was used to count the number of viable and dead cells, then the survival rate and growth curve were achieved; DNA ladder bands were observed by DNA agarose gel electrophoresis; HL-60 cell apoptotic percentage was detected by flow cytometry with PI staining. Results 8-nitrochrysin inhibited proliferation of HL-60 cells in a concentration- dependent manner; 8-nitro- chyrsin posses the inhibitory effect of the proliferation and growth of HL-60 cells; 8-nitrochrysin posses the significantly effect of induce apoptosis of HL-60 cells. Conclusion 8-nitrochyrsin posses the inhibitory effect of the proliferation and growth of HL-60 cells And significantly induce apoptosis of HL-60 cells.

Key words: Human Acute myeloid leukaemia, Chrysin, 8-nitrochyrsin, Apoptosis