关键词: 量子点, 纳米技术, 牙乳头细胞, Smad2

Abstract: Objective To prepare Semiconductor Quantum Dots (QDs)-Smad2 monoclonal antibody Fluorescent probes , to detect nucleation shifting process of Smad2 signal protein in Rat dental papillae cells(RDPCs)stimulated by TGF-β1 with the Fluorescent probes.Methods ① QDs were chemically modified with Smad2 proteins to prepare water soluble QDs-Smad2 monoclonal antibody Fluorescent probes which were purified after the preparation, and detect its related optical properties. ②the location of Smad2 proteins in RDPCs was studied with QDs direct mark method、Smad2 monoclonal antibody direct mark method and direct immunofluorescence imaging. And the properties of Quantum Dots-Smad2 monoclonal antibody Fluorescent probes in RDPCs were detected. ③after RDPCs cultivated by TGF-β1 for 0 hours、12 hours、24 hours ,the dynamic nucleation shifting process of Smad2 signal protein in RDPCs was separately detected with direct immunofluorescence imaging.Result QDs and monoclonal antibody covalently bond to form the Fluorescent probes which could specifically and effectively recognize Smad2 proteins in RDPCs .It is to demonstrate the dynamic nucleation shifting process of Smad2 signal protein in RDPCs. These Fluorescent probes still had good high fluorescence intensity and photostability. conclusion QDs and monoclonal antibody could covalently bond to form the Fluorescent probes with distinct optics character、special immunity recognition capability and the ability of imaging mark the protein in cells for long time .

Key words: Quantum dots, Nanotechnology, Dental papillae cells, Smad2