Abstract: Objective To express the human recombinant NSPc1 protein and prepare specific polyclonal antibody against it. Methods The recombinant expression plasmid pET43.1a-HIS-NSPc1 was made and transformed into E.coli.(BL21), and then the recombinant fusion protein HIS-NSPc1 was expressed and purified. Four New Zealand rabbits were immuniged with purified recombinant HIS-NSPc1 protein as antigen and polyclonal antibodies against NSPc1 were prepared. The specificity of the anti-sera were analyzed by Western Blot. Results The purity of the recombinant NSPc1 protein is up to about 95%. Rabbit against NSPc1 antibodies were obtained. Western blot showed that the antibodies were able to detect both endogenous and/or exogenous NSPc1. Conclusion The NSPc1 polyclonal antibodies prepared by using recombinant NSPc1 protein as antigen has better specificity to NSPc1.It can be used for functional analysis of NSPc1 in vivo and in vitro.