关键词: 斑马鱼, 胰岛β细胞, 转基因, 胰岛素, 荧光蛋白

Abstract: Objective we have established pancreatic β-cell developmet fish model, which showed specific spatial expression patterns in vivo. Methods molecular clone, microinjection, whole embryo in-situ hybridization（WISH）and fluorescence microscopy in living were used to analyze of β-cell development by generation of transgenic zebrafish. Results screening and established pancreatic β cells of transgenic zebrafish, then confirmed that fluorescence protein expression in the same spatiotemporal pattern with endogenous insulin gene to achieve dynamic monitoring islet β-cell development situation in vivo. Conclusion the pancreatic β cells of transgenic zebrafish animal model can be successfully traced pancreatic β cell development in whole level.

Key words: zebrafish, pancreaticβcell, transgenic, insulin, fluorescence protein