联合使用米索前列醇和米非司酮以获取恒河猴胎猴样品

基础医学与临床 ›› 2009, Vol. 29 ›› Issue (9): 908-910.

联合使用米索前列醇和米非司酮以获取恒河猴胎猴样品

赵理罕园园唐东红

中国医学科学院基础医学研究所北京协和医学院基础学院医学分子生物学国家重点实验室中国医学科学院/北京协和医学院医学生物学研究所中国医学科学院/北京协和医学院医学生物学研究所

收稿日期:2009-04-09 修回日期:2009-06-15 出版日期:2009-09-20 发布日期:2009-09-20
通讯作者: 唐东红

Obtaining fetus of rhesus monkey with the combination of misoprostol and mifepristone

Li ZHAO, Yuanyuan HAN, Donghong TANG

National Lab of Medical Molecular Biology, Institute of Basic Medical Science& School of Basic Medicine, CAMS & PUMC Institute of Medical Biology, CAMS&PUMC

Received:2009-04-09 Revised:2009-06-15 Online:2009-09-20 Published:2009-09-20
Contact: Donghong TANG

摘要/Abstract

摘要： 目的建立获取恒河猴早中晚期不同胎龄胎猴样品的新方法。方法通过B超确定恒河猴孕猴怀孕天数后，皮下注射米非司酮，在其阴道后穹窿放置米索前列醇以促使孕猴终止妊娠，获取胎猴样品。通过bishop评分，孕猴终止妊娠率，胎猴脑组织总RNA提取质量评估该新方法效果。结果实验组孕猴的宫颈评分和胎猴三日娩出率均高于对照组（P<0.05），从胎猴猴脑组织中提取的总RNA质量良好。结论联合使用米索前列醇和米非司酮是一种有效而安全的获取恒河猴胎猴样品的新方法。

关键词: 恒河猴, 米非司酮, 米索前列醇

Abstract: Object To establish a safe and effective method to obtain fetus of rhesus monkey. Method The pregnant days of rhesus monkey was identified by B ultrasonography, and the pregnant rhesus monkeys in different stages of pregnant were chosen randomly. Mifepristone was subcutaneously injected to pregnant rhesus while misoprostol was put to their posterior fornix. The effect of this method was evaluated by the results of bishop score ,the rate of pregnancy termination and the quality of total RNA extracted from brain samples of rhesus fetus. Result The scores of bishop score and rates of fetus delivery of experiment group were both higher than control group (P<0.05) ,and the quality of total RNA extracted from brain samples of rhesus fetus are well. Conclusion Combined application of misoprostol and mifepristone can effective and safely obtain samples of rhesus fetus.

Key words: rhesus monkey, mifepristone, misoprostol

赵理罕园园唐东红. 联合使用米索前列醇和米非司酮以获取恒河猴胎猴样品[J]. 基础医学与临床, 2009, 29(9): 908-910.

Li ZHAO; Yuanyuan HAN; Donghong TANG. Obtaining fetus of rhesus monkey with the combination of misoprostol and mifepristone[J]. Basic & Clinical Medicine, 2009, 29(9): 908-910.