下调Talin1抑制肝肺综合征模型大鼠血清诱导的PASMCs迁移

doi:10.16352/j.issn.1001-6325.2025.11.1409

基础医学与临床 ›› 2025, Vol. 45 ›› Issue (11): 1409-1414.doi: 10.16352/j.issn.1001-6325.2025.11.1409

下调Talin1抑制肝肺综合征模型大鼠血清诱导的PASMCs迁移

陈杨¹, 文静¹, 石岚¹, 杨勇², 易斌², 陈林^2*

1.重庆市第七人民医院(重庆理工大学附属中心医院)麻醉科,重庆 400054;
2.陆军军医大学第一附属医院麻醉科,重庆 400038

收稿日期:2024-09-02 修回日期:2025-01-03 出版日期:2025-11-05 发布日期:2025-10-24
通讯作者: ^*chenlin8805@163.com
基金资助:
国家自然科学基金(82270656);重庆市自然科学基金(CSTB2022NSCQ-MSX0218);重庆市教委科技研究计划青年项目(KJQN202401137)

Downregulation of Talin1 inhibits migration of pulmonary arterial smooth muscle cells(PASMCs) induced by serum of rat models with HPS

CHEN Yang¹, WEN Jing¹, SHI Lan¹, YANG Yong², YI Bin², CHEN Lin^2*

1. Department of Anesthesiology, the Seventh People's Hospital of Chongqing (Affiliated Central Hospital of Chongqing University of Technology), Chongqing 400054;
2. Department of Anesthesiology, the First Affiliated Hospital, Army Medical University, Chongqing 400038, China

Received:2024-09-02 Revised:2025-01-03 Online:2025-11-05 Published:2025-10-24
Contact: ^*chenlin8805@163.com

摘要/Abstract

摘要： 目的评价踝蛋白1(Talin1)在肝肺综合征(HPS)大鼠血清诱导大鼠肺动脉平滑肌细胞(PASMCs)迁移中的作用。方法雄性SD大鼠20只,用慢性胆总管结扎法制备HPS大鼠模型,取腹主动脉血样制备血清。将PASMCs接种于6孔板和24孔板,随机分为:对照组(control)和HPS组,用Talin1或对照siRNA转染细胞。分别加入终浓度为5%的正常大鼠血清或HPS大鼠血清。于转染细胞后24 h或细胞孵育24 h(T1),48 h(T2)和72 h(T3)时用Western blot测定PASMCs内Talin1及活性Integin β1的蛋白表达;用Transwell小室法(T1)及划痕实验(T1~3)测定PASMCs的迁移水平。结果与对照组比较,HPS组随刺激时间延长,Talin1蛋白表达上调,PASMCs迁移逐渐增强(P＜0.05);Talin1 siRNA能有效沉默Talin1基因;与对照组比较,HPS组+si对照中活性Integin β1蛋白表达及PASMCs迁移水平增强(P＜0.05);与HPS组+si对照比较,HPS组+siTalin1中活性Integin β1蛋白表达及PASMCs迁移水平明显受抑(P＜0.05)。结论下调Talin1蛋白的表达可抑制HPS大鼠血清诱导的PASMCs迁移及活性Integin β1蛋白的表达。

关键词: 肝肺综合征, 肺动脉平滑肌细胞, 踝蛋白1, 整合素β1

Abstract: Objective To evaluate the role of Talin1 in the migration of rat pulmonary artery smooth muscle cells (PASMCs) induced by the serum from rats with hepatopulmonary syndrome (HPS). Methods Twenty male Sprague-Dawley (SD) rats were used as HPS rat models by chronic common bile duct ligation, the serum was collected from abdominal aorta. PASMCs were seeded in 6-well and 24-well plates and randomly divided into control group and HPS group. The cells were transfected with Talin1 or control siRNA. The normal rat serum or HPS rat serum with a final concentration of 5% was added respectively. At 24 hours after cell transfection or at 24 hours (T1), 48 hours (T2) and 72 hours (T3) of cell incubation, the protein expression of Talin1 and active Integin β1 in PASMCs were determined by Western blot; The migration of PASMCs was measured by Transwell chamber (T1) and scratch assay (T1 to T3). Results Compared to control group, with the extension of the stimulation time in the HPS group, the expression of Talin1 protein was upregulated, and the migration of PASMCs was gradually enhanced (P＜0.05); Talin1 siRNA effectively silenced the Talin1 gene; The expression of active Integin β1 protein and the migration of PASMCs in the HPS group + si control were enhanced (P＜0.05); Compared with the HPS group + si control, the expression of active Integin β1 protein and the migration of PASMCs in the HPS group + siTalin1 were significantly inhibited (P＜0.05). Conclusions Downregulating expression of Talin1 protein inhibits migration of PASMCs and expression of active Integin β1 protein induced by the serum from HPS rats.

Key words: hepatopulmonary syndrome, pulmonary arterial smooth muscle cells, Talin1, Integrin β1

中图分类号:

R563.9

陈杨, 文静, 石岚, 杨勇, 易斌, 陈林. 下调Talin1抑制肝肺综合征模型大鼠血清诱导的PASMCs迁移[J]. 基础医学与临床, 2025, 45(11): 1409-1414.

CHEN Yang, WEN Jing, SHI Lan, YANG Yong, YI Bin, CHEN Lin. Downregulation of Talin1 inhibits migration of pulmonary arterial smooth muscle cells(PASMCs) induced by serum of rat models with HPS[J]. Basic & Clinical Medicine, 2025, 45(11): 1409-1414.

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下调Talin1抑制肝肺综合征模型大鼠血清诱导的PASMCs迁移

Downregulation of Talin1 inhibits migration of pulmonary arterial smooth muscle cells(PASMCs) induced by serum of rat models with HPS

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