银杏叶提取物抑制人胶质瘤细胞系U87增殖和迁移

doi:10.16352/j.issn.1001-6325.2025.09.1144

基础医学与临床 ›› 2025, Vol. 45 ›› Issue (9): 1144-1150.doi: 10.16352/j.issn.1001-6325.2025.09.1144

银杏叶提取物抑制人胶质瘤细胞系U87增殖和迁移

马悦¹, 黄从刚¹, 王远¹, 徐文青², 罗志华^1*

1.武汉市第一医院神经外科,湖北武汉430022;
2.桂林市人民医院心脏中心,广西桂林 541000

收稿日期:2025-03-14 修回日期:2025-05-27 出版日期:2025-09-05 发布日期:2025-08-27
通讯作者: ^*6795173@qq.com
基金资助:
国家自然科学基金青年科学基金(81601076);湖北省卫生健康委员会项目(WJ2019H331);武汉市卫生健康委员会重点项目(WX19A16);武汉市中医药科研项目(WZ24B61)

Ginkgo biloba extract inhibits the proliferation and migration of human glioma cell line U87

MA Yue¹, HUANG Conggang¹, WANG Yuan¹, XU Wenqing², LUO Zhihua^1*

1. Department of Neurosurgery, Wuhan First Hospital, Wuhan 430022;
2. The Heart Center, Guilin People′s Hospital, Guilin 541000, China

Received:2025-03-14 Revised:2025-05-27 Online:2025-09-05 Published:2025-08-27
Contact: ^*6795173@qq.com

摘要/Abstract

摘要： 目的探究银杏叶提取物(GBE)对人胶质瘤细胞系增殖和迁移的影响。方法体外培养胶质瘤细胞系U87,分别采用0、10、50、100 μg/mL的银杏叶提取物干预培养。通过5-乙炔基-2’-脱氧尿苷(EDU)实验检测各组细胞增殖活性;划痕实验检测各组细胞迁移活性;Transwell小室法检测各组细胞侵袭活性;Western blot检测各组上皮细胞-间质转化(EMT)相关蛋白质、磷脂酰肌醇-3-激酶(PI3K)/蛋白激酶B(AKT)通路蛋白和E2F转录因子1(E2F1)蛋白表达水平。加入PI3K/AKT通路激活剂740 Y-P进行回复实验。结果 10、50、100 μg/mL的银杏叶提取物均显著抑制了U87细胞增殖(P＜0.05)、迁移和侵袭活性(P＜0.05),细胞内vimentin、N-cadherin、p-PI3K、p-AKT、E2F1蛋白水平均显著降低(P＜0.05),而E-cadherin、ZO-1蛋白水平均显著升高(P＜0.05)。加入740 Y-P后,银杏叶提取物对U87细胞增殖和转移的抑制作用减弱(P＜0.05),细胞内vimentin、N-cadherin、p-PI3K、p-AKT、E2F1蛋白水平均有所升高(P＜0.05),而E-cadherin、ZO-1蛋白水平均有所降低(P＜0.05)。结论银杏叶提取物可以抑制U87细胞增殖、转移和EMT,这可能与PI3K/AKT/E2F1通路相关。

关键词: 银杏叶提取物, 胶质瘤, PI3K/AKT通路, E2F转录因子1(E2F1)

Abstract: Objective To find potential effect of Ginkgo biloba extract on proliferation and migration of human glioma cell line. Methods Glioma cell line U87 was cultured and incubated with Ginkgo biloba extract at doses of 0, 10, 50, and 100 μg/mL, respectively. The proliferation activity of the cells in each group was detected by 5-ethynyl-2’-deoxyuridine (EDU) experiment, the migration activity of the cells in each group was examined by scratch experiment, the invasion activity of the cells in each group was detected by Transwell experiment and the expression of epithelial-mesenchymal transition (EMT)-related proteins, phosphatidylinositol-3-kinase(PI3K)/protein kinase B (AKT) signaling pathway proteins and E2F transcription factor 1(E2F1)protein in each group of the cells were detected by Western blot. Reply experiment was added with PI3K/AKT pathway activator 740 Y-P. Results Ginkgo biloba extract at concentrations of 10, 50, and 100 μg/mL significantly inhibited the proliferation, migration, and invasion of U87 cells(P＜0.05). The protein level of vimentin, N-cadherin, p-PI3K, p-AKT and E2F1 was significantly decreased(P＜0.05), while the protein level of E-cadherin and ZO-1 was significantly increased(P＜0.05). After addition of 740 Y-P into the cultural system, the inhibitory effect of Ginkgo biloba extract on the proliferation and metastasis of U87 cells was inhibited and the protein level of vimentin, N-cadherin, p-PI3K, p-AKT, and E2F1 increased(P＜0.05), while the protein level of E-cadherin and ZO-1 was decreased(P＜0.05). Conclusions Ginkgo biloba extract may inhibit proliferation, migration and EMT of U87 cells, which is potentially related to the PI3K/AKT/E2F1 pathway.

Key words: Ginkgo biloba extract, glioma, PI3K/AKT pathway, E2F transcription factor 1(E2F1)

中图分类号:

R739.4

马悦, 黄从刚, 王远, 徐文青, 罗志华. 银杏叶提取物抑制人胶质瘤细胞系U87增殖和迁移[J]. 基础医学与临床, 2025, 45(9): 1144-1150.

MA Yue, HUANG Conggang, WANG Yuan, XU Wenqing, LUO Zhihua. Ginkgo biloba extract inhibits the proliferation and migration of human glioma cell line U87[J]. Basic & Clinical Medicine, 2025, 45(9): 1144-1150.

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银杏叶提取物抑制人胶质瘤细胞系U87增殖和迁移

Ginkgo biloba extract inhibits the proliferation and migration of human glioma cell line U87

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